Results 31 to 40 of about 23,186 (181)

Affinity chromatography of dihydrofolate reductase [PDF]

Biochemical Journal, 1971
1. Dihydrofolate reductase was purified from Lactobacillus casei MTX/R, and studied on affinity columns containing folic acid and methotrexate. Two forms of the enzyme were interconverted by incubation with substrates. 2. Affinity columns were prepared from agarose activated with cyanogen bromide and coupled with 1,6-diaminohexane.
P C, Newbold, N G, Harding
openaire   +2 more sources

Overview of Encapsulated Lysine and Methionine and Their Impacts on Transition Cow Performance and Health

Animal Research and One Health, EarlyView.
The transition period in dairy cows, spanning 3 weeks before and after calving, is a critical phase characterized by increased nutrient demands, reduced dry matter intake (DMI), and elevated risk of metabolic disorders such as negative nutrient balance (NNB), lipolysis, proteolysis, and oxidative stress.
Mohammed S. Seleem, Samy A. Elsaadawy, Zhaohai Wu, Lu Ma, Todd R. Callaway, Dengpan Bu   +5 more
wiley   +1 more source

Inosine triphosphate pyrophosphatase activity as a potential predictor of methotrexate remission in juvenile idiopathic arthritis

Arthritis &Rheumatology, Accepted Article.
Background Methotrexate (MTX) is the first‐line therapy for juvenile idiopathic arthritis (JIA), but up to 40% of patients don't respond. Low inosine‐triphosphate‐pyrophosphatase (ITPA) activity has been associated with reduced clinical remission. We investigated ITPA role and the underlying mechanisms in vitro.
Sofia Sindici Forgiarini, Marianna Lucafò, Debora Curci, Davide Selvestrel, Valentina Moressa, Sofia Pagarin, Barbara Bellich, Martina Franzin, Alberto Tommasini, Mara L. Becker, Laura B. Ramsey, Susan D. Thompson, Carl D. Langefeld, Federica Corba, Edoardo Marrani, Gabriele Simonini, Gabriele Stocco, Giuliana Decorti, Andrea Taddio, Serena Pastore   +19 more
wiley   +1 more source

The power of many: when genetics met yeasts and high‐throughput

Biological Reviews, EarlyView.
ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley   +1 more source

Type 2 antifolates in the chemotherapy of falciparum malaria

Journal of the Brazilian Chemical Society, 2002
About 40% of the world population is exposed to malaria, which results in the death of over 2 million people per year in Africa, Latin America, Southern Asia and Oceania.
Delfino Reinaldo T., Santos-Filho Osvaldo A., Figueroa-Villar José D.   +2 more
doaj  

Effect of Sunflower (Helianthus annuus L.) Seeds Treatment by Diode Laser Radiation in Seedlings and Calli Growth

مجلة مركز بحوث التقنيات الاحيائية, 2015
The effect of the exposure of sunflower (Helianthus annuus L.) seeds to red light laser radiation with 650 nm 50 mw/cm2 by diode laser in germination and growth of seedlings and calli had been studied.
Sara N. Ghanem Ghanem, Sajida A. Abood Abood   +1 more
doaj   +1 more source

Vibrational Structure of Dihydrofolate Bound to R67 Dihydrofolate Reductase [PDF]

Journal of Biological Chemistry, 2001
R67 is a Type II dihydrofolate reductase (DHFR) that catalyzes the reduction of dihydrofolate (DHF) to tetrahydrofolate by facilitating the addition of a proton to N5 of DHF and the transfer of a hydride ion from NADPH to C6. Because this enzyme is a plasmid-encoded DHFR from trimethoprim-resistant bacteria, extensive studies on R67 with various ...
H, Deng, R, Callender, E, Howell
openaire   +2 more sources

Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐CLOVER establishes enhanced CHO platforms for cell line development

Biotechnology Progress, EarlyView.
This study established the first creation of a commercially available double GS knockout (DKO) from a single KO in CHO‐K1 cells, called CleanCut GS CHO using Cas‐CLOVER. When combined with the Harbor‐IN transposase system, CleanCut GS CHO cells demonstrated a highly antibody productivity.
Cintia Gomez Limia, Valeriya Steffey, Hsueh Che Cheng, Daniel Machado, Thomas Hart, Mark Cameron McHargue, Corey Brizzee, Jack Crawford   +7 more
wiley   +1 more source

Antimalarial flavonoid glycoside from Carica papaya with inhibitory potential against Plasmodium falciparum dihydrofolate reductase thymidylate synthase: an in-silico study

Universa Medicina
BACKGROUND Carica papaya is traditionally used to treat malaria. The mechanism of action of the active constituents may be determined by molecular docking.
Dewa Ayu Agus Sri Laksemi, I Dewa Ayu Inten Dwi Primayanti, I Wayan Surudarma, Putu Ayu Asri Damayanti, Ni Made Pitri Susanti   +4 more
doaj   +1 more source

Metformin sensitises hepatocarcinoma cells to methotrexate by targeting dihydrofolate reductase

Cell Death and Disease, 2021
Metformin, the first-line drug for type II diabetes, has recently been considered an anticancer agent. However, the molecular target and underlying mechanism of metformin’s anti-cancer effects remain largely unclear.
Yinghui Wang, Hui Lu, Linchong Sun, Xin Chen, Haoran Wei, Caixia Suo, Junru Feng, Mengqiu Yuan, Shengqi Shen, Weidong Jia, Ying Wang, Huafeng Zhang, Zijun Li, Xiuying Zhong, Ping Gao   +14 more
doaj   +1 more source