Results 41 to 50 of about 45,192 (224)

Trypanosoma brucei DHRF-TS revisited:characterisation of a bifunctional and highly unstable recombinant dihydrofolate reductase-thymidylate synthase [PDF]

, 2016
Bifunctional dihydrofolate reductase–thymidylate synthase (DHFR-TS) is a chemically and genetically validated target in African trypanosomes, causative agents of sleeping sickness in humans and nagana in cattle.
A Dawson, A El Fadili, A Khodursky, AJ Wahba, AJ Wahba, AL Jackman, Alan H. Fairlamb, B Nare, BJ Dolnick, C Fraser-Lhostis, C Shih, CJ Allegra, CW Carreras, D Spinks, DC Jones, E Wirtz, EJ Shanks, EL White, F Gamarro, FW Studier, H Zhang, Han B. Ong, HB Ong, J Phan, J Yuvaniyama, JA Frearson, Jayne Raper, JF Morrison, JM Blaney, JR Appleman, K Al Chalabi, KY Han, LM Changchien, LS Torrie, M Berriman, M Hekmat-Nejad, Marc W. Gibson, MM Bradford, N Greig, N Sienkiewicz, N Sienkiewicz, Natasha Sienkiewicz, ND Brunn, P Prapunwattana, P Reche, PH Liang, R Ferone, R Grumont, RA Copeland, RC Jackson, RD Walter, RJ Bonney, RJ Cisneros, S Shallom, SA Margosiak, SE Webber, SF Chowdhury, SI Ahmad, Simon Dewar, SM Lanham, TC Eadsforth, TD Meek, TW Synold, Y Cheng   +63 more
core   +3 more sources

Tales of Dihydrofolate Binding to R67 Dihydrofolate Reductase

Biochemistry, 2015
Homotetrameric R67 dihydrofolate reductase possesses 222 symmetry and a single active site pore. This situation results in a promiscuous binding site that accommodates either the substrate, dihydrofolate (DHF), or the cofactor, NADPH. NADPH interacts more directly with the protein as it is larger than the substrate.
Duff, Michael R., Chopra, Shaileja, Strader, Michael Brad, Agarwal, Pratul K., Howell, Elizabeth E.   +4 more
openaire   +2 more sources

eQTL Meta‐Analysis Reveals Conserved and Population‐Specific Regulatory Variation Underlying Nutritional Trait Evolution and Domestication in Tomato

Advanced Science, EarlyView.
A comprehensive meta‐analysis of expression quantitative trait loci (eQTLs) across five diverse tomato populations reveals a high‐resolution atlas of transcriptional regulation and uncovers conserved and population‐specific regulatory architectures underlying fruit nutritional quality traits, including flavonoids, sugars, organic acids, carotenoids ...
Jiantao Zhao, Xin Wang, Jing Zhang, Ryan McQuinn, Je Min Lee, Itay Gonda, Ari Feder, Yimin Xu, Baike Wang, Qinghui Yu, Denise M. Tieman, Sanwen Huang, Harry Klee, James J. Giovannoni, Zhangjun Fei   +14 more
wiley   +1 more source

A silent sulfonamide-resistant microbial world: The curious case of missing DHPS in candidate phyla radiation

Journal of Global Antimicrobial Resistance
Candidate phyla radiation (CPR) microbes exhibit minimal genomes, episymbiotic/parasitic lifestyles, and metabolic dependencies on host cells. In this study, we look for the presence of dihydropteroate synthase (DHPS) and dihydrofolate reductase, key ...
Riyad Razzouk, Adel Azour, Jean-Marc Rolain, Fadi Bittar   +3 more
doaj   +1 more source

Orthogonal Ionic Liquid‐Based Extraction Strategy Enables Amyloid‐Specific Profiling of Aggregate Proteome

Advanced Science, EarlyView.
An orthogonal ionic‐liquid extraction (Orth‐iEA) enables selective isolation of amyloid fibrils. TMGBF4 disrupts hydrogen‐bonded β‐sheet networks to solubilize amyloid aggregates, whereas C12ImCl interacts with hydrophobic regions of non‐amyloid proteins.
Shiying Zheng, Ye Liu, Bowen Zhong, Huiying Chu, Zhou Gong, Baofeng Zhao, Mengchun Cheng, Zhen Liang, Yukui Zhang, Qun Zhao, Lihua Zhang   +10 more
wiley   +1 more source

Combined gene deletion of dihydrofolate reductase-thymidylate synthase and pteridine reductase in Leishmania infantum.

PLoS Neglected Tropical Diseases, 2021
Our understanding of folate metabolism in Leishmania has greatly benefited from studies of resistance to the inhibitor methotrexate (MTX). Folates are reduced in Leishmania by the bifunctional dihydrofolate reductase thymidylate synthase (DHFR-TS) and by
Arijit Bhattacharya, Philippe Leprohon, Marc Ouellette   +2 more
doaj   +1 more source

Prediction of Inhibition Activity of Dihydrofolate Reductase Inhibitors With Multivariate Adaptive Regression Splines

IEEE Access, 2023
Dihydrofolate reductase (DHFR) enzyme is a crucial component of cell growth and proliferation in the human body, making it an important target for treating cancer diseases.
Zanib Qayyum, Tahir Mehmood, Laila A. Al-Essa   +2 more
doaj   +1 more source

Therapeutic Efficacy of Sulfadoxine-Pyrimethamine and Prevalence of Resistance Markers in Tanzania Prior to Revision of Malaria Treatment Policy: Plasmodium Falciparum Dihydrofolate Reductase and Dihydropteroate Synthase Mutations in Monitoring in Vivo Resistance. [PDF]

, 2004
Prior to the 2001 malarial treatment policy change in Tanzania, we conducted trials to assess the efficacy of sulfadoxine-pyrimethamine (SP) and the usefulness of molecular markers in monitoring resistance.
Abdulla, Salim, Beck, Hans-Peter, Kataraihya, Johannes, Lemnge, Martha, Malisa, Allen, Mshinda, Hassan, Mugittu, Kefas, Mwita, Alex, Ndejembi, Modesta, Nkya, Watoky, Premji, Zulfikar   +10 more
core   +1 more source

A designed phenylalanyl-tRNA synthetase variant allows efficient in vivo incorporation of aryl ketone functionality into proteins [PDF]

, 2002
Incorporation of non-natural amino acids into proteins in vivo expands the scope of protein synthesis and design. p-Acetylphenylalanine was incorporated into recombinant dihydrofolate reductase (DHFR) in Escherichia coli via a computationally designed ...
Carrico, Isaac S., Datta, Deepshikha, Mayo, Stephen L., Tirrell, David A., Wang, Pin   +4 more
core   +2 more sources

Metabolism‐Based Biomarkers for Rapid Phenotypic Antibiotic Susceptibility Testing

Advanced Science, EarlyView.
Metabolic differences emerging after antibiotic exposure provide rapid phenotypic signals of susceptibility and resistance. This review summarizes metabolic biomarkers for rapid phenotypic AST, associated with nutrient uptake, respiratory activity, metabolic reprogramming, and enzymatic function. ABSTRACT The accelerating global crisis of antimicrobial
Sha Yu, Bayinqiaoge, Xi Lu, Xin Wang, Rongfeng Wang, Yi Li, Shi‐Yang Tang, Chengchen Zhang   +7 more
wiley   +1 more source