Results 61 to 70 of about 125,417 (229)

Antimalarial flavonoid glycoside from Carica papaya with inhibitory potential against Plasmodium falciparum dihydrofolate reductase thymidylate synthase: an in-silico study

Universa Medicina
BACKGROUND Carica papaya is traditionally used to treat malaria. The mechanism of action of the active constituents may be determined by molecular docking.
Dewa Ayu Agus Sri Laksemi, I Dewa Ayu Inten Dwi Primayanti, I Wayan Surudarma, Putu Ayu Asri Damayanti, Ni Made Pitri Susanti   +4 more
doaj   +1 more source

Temporal Hydrogen‐Bond Network Analysis Reveals Substrate‐Directed Connectivity in Dihydrofolate Reductase

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Hydrogen‐bond networks are central to protein function, but most network analyses rely on static representations that neglect how interactions evolve in time. Here, we introduce a framework that combines instantaneous and temporal graph analysis of hydrogen‐bond networks derived from molecular dynamics (MD) trajectories to quantify ligand ...
Tandac F. Guclu, Canan Atilgan, Ali Rana Atilgan   +2 more
wiley   +1 more source

Cloning, epression and purification of mycobacterial dihydrofolate reductase [PDF]

, 2014
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kateřina Šedivá Supervisor: Mgr. Eva Novotná, Ph.D.
Šedivá, Kateřina
core  

Substrate channeling between the human dihydrofolate reductase and thymidylate synthase

, 2016
In vivo, as an advanced catalytic strategy, transient non-covalently bound multi-enzyme complexes can be formed to facilitate the relay of substrates, i. e.
McCammon, J Andrew, Wang, Nuo
core   +1 more source

High resistance of Plasmodium falciparum to sulphadoxine/pyrimethamine in northern Tanzania and the emergence of dhps resistance mutation at Codon 581. [PDF]

, 2009
BACKGROUND: Sulphadoxine-pyrimethamine (SP) a widely used treatment for uncomplicated malaria and recommended for intermittent preventive treatment of malaria in pregnancy, is being investigated for intermittent preventive treatment of malaria in infants
Gesase Samwel, Daniel Chandramohan, Mosha Jacklin F., Mandia, Victor, Joho, Angel, Victor Mandia, Martha Lemnge, Greenwood, Brian, Samwel Gesase, Gesase, Samwel, Chandramohan Daniel, Mosha Frank W., Savael, Zacharia, Ord, Rosalynn, Roper, Cally, Angel Joho, Madebe, Rashid, Greenwood Brian, Hashim Ramadhan, Frank W. Mosha, Mrema Hedwiga, Inbarani Naidoo, Chandramohan, Daniel, Zacharia Savael, Mapunda Ephraim, Mosha, Frank W, Lemnge Martha, Madebe Rashid, Rosalynn Ord, Mapunda, Ephraim, Ephraim Mapunda, Roly D Gosling, Naidoo Inbarani, Frank W Mosha, Hashim, Ramadhan, Cally Roper, Mandia Victor, Gosling, Roly D, Victor M, Jacklin F. Mosha, Gosling Roly D., Lemnge, Martha, Ord Rosalynn, Hedwiga Mrema, Brian Greenwood, Mrema, Hedwiga, Roly D. Gosling, Roper Cally, Jacklin F Mosha, Ramadhan Hashim, Rashid Madebe, Mosha, Jacklin F, Naidoo, Inbarani, Savael Zacharia, Joho Angel   +54 more
core   +2 more sources

Metformin sensitises hepatocarcinoma cells to methotrexate by targeting dihydrofolate reductase

Cell Death and Disease, 2021
Metformin, the first-line drug for type II diabetes, has recently been considered an anticancer agent. However, the molecular target and underlying mechanism of metformin’s anti-cancer effects remain largely unclear.
Yinghui Wang, Hui Lu, Linchong Sun, Xin Chen, Haoran Wei, Caixia Suo, Junru Feng, Mengqiu Yuan, Shengqi Shen, Weidong Jia, Ying Wang, Huafeng Zhang, Zijun Li, Xiuying Zhong, Ping Gao   +14 more
doaj   +1 more source

Clinical predictors of response to methotrexate in patients with rheumatoid arthritis: A systematic review

Rheumatology &Autoimmunity, EarlyView.
This review integrates clinical, immunological, genetic, and multi‐omic evidence, highlighting dynamic inflammatory and adenosine‐pathway biomarkers and reinforcing treat‐to‐target principles as the most actionable framework for personalized methotrexate therapy.
Joana Ramos Rodrigues, Helena Margarida de Miranda Lemos Romão Donato, Soraia Raquel Loureiro Azevedo, Luís Miguel da Silva Pires, Luís Pedro Bolotinha de Sousa Inês, Manuel Augusto Nunes Vicente Passos Morgado, Ana Filipa de Sousa Pestana Mourão   +6 more
wiley   +1 more source

Transgenic selection and underlying mechanisms in apicomplexan parasites

The FEBS Journal, EarlyView.
Transgenic selection markers have driven genetic engineering in apicomplexans, enabling precise, iterative experiments. This review discusses mechanistic details of drug selection markers, strategies for marker recycling, and practical considerations for several clinically relevant parasites.
Swaroop Peddiraju, Rakhee Lohia, Nishith Gupta   +2 more
wiley   +1 more source

Dihydropteridine Reductase as an Alternative to Dihydrofolate Reductase for Synthesis of Tetrahydrofolate in Thermus thermophilus

, 2004
A strategy devised to isolate a gene coding for a dihydrofolate reductase from Thermus thermophilus DNA delivered only clones harboring instead a gene (the T.
Legrain, Christiane, Glansdorff, Nicolas, Wilquet, Valérie, Van De Casteele, Christine, Gigot, Daniel   +4 more
core   +1 more source

Blocking protein quality control degradation leads to structural stabilization of DHFR indel variants

The FEBS Journal, EarlyView.
Blocking protein quality control degradation by deleting the E3 ligases Ubr1 or San1 stabilizes many DHFR insertion and deletion variants that are otherwise partially unfolded. These hypomorphic variants regain folding at lower temperatures or upon methotrexate binding, revealing that PQC degradation can remove proteins that are still functionally ...
Sven Larsen‐Ledet, Caroline H. Suhr, Sarah Gersing, Celeste M. Hackney, Amelie Stein, Kaare Teilum, Rasmus Hartmann‐Petersen   +6 more
wiley   +1 more source