Results 31 to 40 of about 23,665 (170)

A Two-Enzyme Adaptive Unit within Bacterial Folate Metabolism

Cell Reports, 2019
Summary: Enzyme function and evolution are influenced by the larger context of a metabolic pathway. Deleterious mutations or perturbations in one enzyme can often be compensated by mutations to others.
Andrew F. Schober, Andrew D. Mathis, Christine Ingle, Junyoung O. Park, Li Chen, Joshua D. Rabinowitz, Ivan Junier, Olivier Rivoire, Kimberly A. Reynolds   +8 more
doaj   +1 more source

Combined gene deletion of dihydrofolate reductase-thymidylate synthase and pteridine reductase in Leishmania infantum.

PLoS Neglected Tropical Diseases, 2021
Our understanding of folate metabolism in Leishmania has greatly benefited from studies of resistance to the inhibitor methotrexate (MTX). Folates are reduced in Leishmania by the bifunctional dihydrofolate reductase thymidylate synthase (DHFR-TS) and by
Arijit Bhattacharya, Philippe Leprohon, Marc Ouellette   +2 more
doaj   +1 more source

Cinnamomum zeylanicum Extract and its Bioactive Component Cinnamaldehyde Show Anti-Tumor Effects via Inhibition of Multiple Cellular Pathways

Frontiers in Pharmacology, 2022
Cinnamomum zeylanicum is a tropical plant with traditional medicinal significance that possesses antimicrobial, antifungal, anti-parasitic, and anti-tumor properties.
Sadhna Aggarwal, Kanchan Bhadana, Baldeep Singh, Meenakshi Rawat, Taj Mohammad, Lamya Ahmed Al-Keridis, Nawaf Alshammari, Md. Imtaiyaz Hassan, Satya N. Das   +8 more
doaj   +1 more source

Precision Chemistry for Protein Lysine Modification

Chemistry – A European Journal, EarlyView.
Selective modification of lysine residues is challenging due to their similar intrinsic reactivity. Inspired by enzymatic recognition, ligand‐guided electrophiles enable site‐selective labeling and functionalization, while ligand‐guided catalyses achieve regioselective installation of bio‐relevant post‐translational modifications.
Mayu Onoda, Motomu Kanai
wiley   +1 more source

Assessment of Attainable Distances in Proteins by Through‐Space 19F–19F Couplings

Chemistry – A European Journal, EarlyView.
De novo synthesized difluorinated tryptophan analogues are site‐specifically incorporated into Cyclophilin A (CypA). 19F–19F NOESY build‐up experiments are recorded for each labeled protein, and the distances derived from cross‐relaxation rates are compared to reference distances.
Iker Hernández, In‐Ja L. Byeon, Christina M. Monnie, Vadim A. Soloshonok, Aitor Landa, Mikel Oiarbide, Angela M. Gronenborn   +6 more
wiley   +1 more source

Prediction of Inhibition Activity of Dihydrofolate Reductase Inhibitors With Multivariate Adaptive Regression Splines

IEEE Access, 2023
Dihydrofolate reductase (DHFR) enzyme is a crucial component of cell growth and proliferation in the human body, making it an important target for treating cancer diseases.
Zanib Qayyum, Tahir Mehmood, Laila A. Al-Essa   +2 more
doaj   +1 more source

Vibrational Structure of Dihydrofolate Bound to R67 Dihydrofolate Reductase [PDF]

Journal of Biological Chemistry, 2001
R67 is a Type II dihydrofolate reductase (DHFR) that catalyzes the reduction of dihydrofolate (DHF) to tetrahydrofolate by facilitating the addition of a proton to N5 of DHF and the transfer of a hydride ion from NADPH to C6. Because this enzyme is a plasmid-encoded DHFR from trimethoprim-resistant bacteria, extensive studies on R67 with various ...
H, Deng, R, Callender, E, Howell
openaire   +2 more sources

Inherited metabolic epilepsies–established diseases, new approaches

Epilepsia Open, EarlyView.
Abstract Inherited metabolic epilepsies (IMEs) represent the inherited metabolic disorders (IMDs) in which epilepsy is a prevailing component, often determining other neurodevelopmental outcomes associated with the disorder. The different metabolic pathways affected by individual IMEs are the basis of their rarity and heterogeneity.
Itay Tokatly Latzer, Phillip L. Pearl
wiley   +1 more source

Extraction of Antimicrobial Peptides From Fava Bean Protein Hydrolyzate by Phosphorylated Biochar

Food Safety and Health, EarlyView.
Antimicrobial peptides were extracted by functional biochar in one step. The enriched peptides demonstrated significantly enhanced antibacterial activity. ABSTRACT The extraction of antimicrobial peptides (AMPs) from plant proteins remains challenging due to their low abundance, structural diversity, and the need for mild, efficient, and selective ...
Xiang‐Yu Huang, Hao‐Ran Liu, Rong Guo, Li Ding, Li Wen, Xu‐Feng Wang, Zhou Xu, Yun‐Hui Cheng, Mao‐Long Chen   +8 more
wiley   +1 more source

Detecting Antibiotic Resistance in Strains of Selected Gram‐Negative Bacterial Species Using Raman Spectroscopy

Journal of Raman Spectroscopy, EarlyView.
Raman spectroscopy successfully identified antibiotic‐resistant Gram‐negative strains. Resistant strains of four bacterial species were discriminated from sensitive ones with 99.1% accuracy using PLS regression. The method allows for rapid, label‐free detection of antibiotic resistance.
Fernanda Sant Ana de Siqueira e Oliveira, Adriano Moraes da Silva, Marcos Tadeu T. Pacheco, Landulfo Silveira Jr.   +3 more
wiley   +1 more source