Results 11 to 20 of about 125,417 (229)

A single-domain protein catenane of dihydrofolate reductase. [PDF]

Natl Sci Rev, 2023
A single-domain protein catenane refers to two mechanically interlocked polypeptide rings that fold synergistically into a compact and integrated structure, which is extremely rare in nature.
Fang J, Li T, Lee J, Im D, Xu L, Liu Y, Seo J, Zhang WB.   +7 more
europepmc   +4 more sources

Dynamics of immobilized and native Escherichia coli dihydrofolate reductase by quasielastic neutron scattering [PDF]

, 2006
The internal dynamics of native and immobilized Escherichia coli dihydrofolate reductase (DHFR) have been examined using incoherent quasielastic neutron scattering.
Smith, J.C., Ollivier, Jacques, Kurkal, V., Monk, C., Oettl, M., Finney, J.L., Tehei, Moeava, Monk, Colin R., Kurkal, Vandana, Smith, Jeremy C., Tehei, M., Finney, John L., Ollivier, J., Oettl, Martin, Daniel, R.M., Daniel, Roy M.   +15 more
core   +1 more source

Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa. [PDF]

, 2003
The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine /pyrimethamine (SP) resistance.
Watkins, W, Kimani, S, Kimani Serah, Kokwaro, Gilbert, Nzila, A, Mutabingwa, T, Kevin Marsh, Theonest Mutabingwa, Serah Kimani, Mutabingwa, Theonest, Gilbert Kokwaro, Nzila Alexis, Ochong, Edwin, Nzila, Alexis, Edwin Ochong, Kokwaro Gilbert, Kimani, Serah, Marsh Kevin, Marsh, K, Ochong Edwin, Alexis Nzila, Watkins, William, William Watkins, Kokwaro, G, Watkins William, Marsh, Kevin, Mutabingwa Theonest, Ochong, E   +27 more
core   +1 more source

DHFR2 RNA directly regulates dihydrofolate reductase and its expression level impacts folate one carbon metabolism. [PDF]

FASEB J
Dihydrofolate reductase activity is required in One Carbon Metabolism to ensure that the biologically active form of folate, tetrahydrofolate, is replenished and available as an enzyme cofactor for numerous cellular reactions, including purine and ...
Drago P, Bookey N, Leung KY, Henry M, Meleady P, Greene NDE, Parle-McDermott A.   +6 more
europepmc   +2 more sources

Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania. [PDF]

, 2006
BACKGROUND\ud \ud Sulphadoxine-pyrimethamine (SP) has been and is currently used for treatment of uncomplicated Plasmodium falciparum malaria in many African countries.
Mbugi, Erasto V, Balthazary Sakurani T, Malisa, Allen L, Mbugi Erasto V, Allen L Malisa, Nyambo, Thomas B, Nyambo, T.B., Mutayoba, B.M., Mutayoba Benezeth M, Nyambo Thomas B, Balthazary, Sakurani T, Erasto V Mbugi, Balthazary, S.T., Hassan Mshinda, Mshinda, H., Mshinda Hassan, Benezeth M Mutayoba, Sakurani T Balthazary, Malisa Allen L, Mutayoba, Benezeth M, Mbugi, E.V., Thomas B Nyambo, Mshinda, Hassan, Malisa, A.L.   +23 more
core   +1 more source

Epidemic of Plasmodium falciparum malaria involving substandard antimalarial drugs, Pakistan, 2003. [PDF]

, 2009
Because of instability in eastern Afghanistan, new refugees crossed into the federally administered tribal areas of northwestern Pakistan in 2002. In 2003, we investigated an epidemic of Plasmodium falciparum malaria in 1 of the camps.
Mark Rowland, Rowland, Mark, Kate Kolaczinski, Rosalynn L. Ord, Kolaczinski, Kate, Kaur, Harpakash, Harpakash Kaur, Toby Leslie, Mohammed, Nasir, Leslie, Toby, Nasir Mohammed, Toby Leslie, Ord, Rosalynn L   +12 more
core   +1 more source

Differences in Thermal Structural Changes and Melting Between Mesophilic and Thermophilic Dihydrofolate Reductase Enzymes [PDF]

, 2020
A key aspect of life\u27s evolution on Earth is the adaptation of proteins to be stable and work in a very wide range of temperature conditions. A detailed understanding of the associated molecular mechanisms would also help to design enzymes optimized ...
Guillaume, Stirnemann, Irene, Maffucci, Damien, Laage, Fabio, Sterpone   +3 more
core   +1 more source

Affinity chromatography of dihydrofolate reductase [PDF]

Biochemical Journal, 1971
1. Dihydrofolate reductase was purified from Lactobacillus casei MTX/R, and studied on affinity columns containing folic acid and methotrexate. Two forms of the enzyme were interconverted by incubation with substrates. 2. Affinity columns were prepared from agarose activated with cyanogen bromide and coupled with 1,6-diaminohexane.
P C, Newbold, N G, Harding
openaire   +2 more sources

Mitochondrial Protein Import [PDF]

, 1987
The role of nucleoside triphosphates (NTPs) in mitochondrial protein import was investigated with the precursors of N. crassa ADP/ATP carrier, F1-ATPase subunit β, F0-ATPase subunit 9, and fusion proteins between subunit 9 and mouse dihydrofolate ...
Pfanner, Nikolaus, Tropschug, Maximilian, Neupert, Walter   +2 more
core   +1 more source

Tales of Dihydrofolate Binding to R67 Dihydrofolate Reductase

Biochemistry, 2015
Homotetrameric R67 dihydrofolate reductase possesses 222 symmetry and a single active site pore. This situation results in a promiscuous binding site that accommodates either the substrate, dihydrofolate (DHF), or the cofactor, NADPH. NADPH interacts more directly with the protein as it is larger than the substrate.
Duff, Michael R., Chopra, Shaileja, Strader, Michael Brad, Agarwal, Pratul K., Howell, Elizabeth E.   +4 more
openaire   +2 more sources