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Advances in directed molecular evolution of reporter genes
Critical Reviews in Biotechnology, 2011Many reporter genes, such as gfp, gusA, and lacZ, are widely used for research into plants, animals, and microorganisms. Reporter genes, which offer high levels of sensitivity and convenience of detection, have been utilized in transgenic technology, promoter analysis, drug screening, and other areas.
Ai-Sheng, Xiong +5 more
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Molecular system identification for enzyme directed evolution and design
The Journal of Chemical Physics, 2017The rational design of chemical catalysts requires methods for the measurement of free energy differences in the catalytic mechanism for any given catalyst Hamiltonian. The scope of experimental learning algorithms that can be applied to catalyst design would also be expanded by the availability of such methods.
Xiangying, Guan, Raj, Chakrabarti
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Molecular Evolution Directs Protein Translation Using Unnatural Amino Acids
Current Protocols in Chemical Biology, 2015AbstractUnnatural amino acids have in recent years established their importance in a wide range of fields, from pharmaceuticals to polymer science. Unnatural amino acids can increase the number of chemical groups within proteins and thus expand or enhance biological function.
Vanessa E, Cox, Eric A, Gaucher
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Detection of biological threats. A challenge for directed molecular evolution
Journal of Microbiological Methods, 2004The probe technique originated from early attempts of Anton van Leeuwenhoek to contrast microorganisms under the microscope using plant juices, successful staining of tubercle bacilli with synthetic dyes by Paul Ehrlich and discovery of a stain for differentiation of gram-positive and gram-negative bacteria by Hans Christian Gram.
Valery A, Petrenko, Iryna B, Sorokulova
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Creation of novel costimulatory ligands through directed molecular evolution
Trends in Immunology, 2002In physiologically relevant situations, activation of naive T cells is thought to require two signals, an antigen-specific signal mediated through the T-cell receptor (signal 1) and an antigen-independent costimulatory signal mediated through activating receptors also expressed on T cells (signal 2).
Jan Woraratanadharm, Mark Rubinstein
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Harnessing Noncovalent Interactions for a Directed Evolution of a Six-Component Molecular Crystal
The Journal of Physical Chemistry B, 2021Building up on weak orthogonal interactions in supramolecular chemistry, a six-component crystal is designed. Using five distinctly different noncovalent forces, namely, hydrogen bonding, halogen bonding, cation-π, anion-π, and ion-pair interactions, three six-component crystals were designed with crown-ether (I), thiourea (II), 2,3,5,6-tetrafluoro-1,4-
Sucharita Mandal +4 more
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Natural selection directing molecular evolution in vertebrate viral sensors
Developmental & Comparative ImmunologyDiseases caused by pathogens contribute to molecular adaptations in host immunity. Variety of viral pathogens challenging animal immunity can drive positive selection diversifying receptors recognising the infections. However, whether distinct virus sensing systems differ across animals in their evolutionary modes remains unclear. Our review provides a
Balraj Melepat, Tao Li, Michal Vinkler
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[Directed molecular evolution of antibodies].
Journal de la Societe de biologie, 2007Antibodies play a key role in the immune system, are characterized by a homogeneous overall structure and by their ability to interact with an almost unlimited number of compounds. Encoded by a fixed number of genes, they acquire their specificity and affinity of recognition after a succession of genetic recombination and molecular mutation processes ...
Olivier, Dubreuil +4 more
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Mutant Library Construction in Directed Molecular Evolution: Casting a Wider Net
Molecular Biotechnology, 2006Directed molecular evolution imitates the natural selection process in the laboratory to find mutant proteins with improved properties in the expected aspects by exploring the encoding sequence space. The success of directed molecular evolution experiment depends on the quality of artificially prepared mutant libraries and the availability of ...
Tian-Wen, Wang +5 more
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Diverse Plasmid DNA Vectors by Directed Molecular Evolution of Cytomegalovirus Promoters
Human Gene Therapy, 2005Genetic vaccinations, gene therapy, and manufacturing of therapeutic proteins would benefit from promoter sequences that provide improved or prolonged expression levels. The cytomegalovirus (CMV) promoter is one of the most potent promoters known to date, and no previous examples of improved activity of this promoter by sequence mutagenesis have been ...
Anne, Wright +7 more
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