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Harnessing Noncovalent Interactions for a Directed Evolution of a Six-Component Molecular Crystal
The Journal of Physical Chemistry B, 2021Building up on weak orthogonal interactions in supramolecular chemistry, a six-component crystal is designed. Using five distinctly different noncovalent forces, namely, hydrogen bonding, halogen bonding, cation-π, anion-π, and ion-pair interactions, three six-component crystals were designed with crown-ether (I), thiourea (II), 2,3,5,6-tetrafluoro-1,4-
Sucharita Mandal +4 more
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Mutant Library Construction in Directed Molecular Evolution: Casting a Wider Net
Molecular Biotechnology, 2006Directed molecular evolution imitates the natural selection process in the laboratory to find mutant proteins with improved properties in the expected aspects by exploring the encoding sequence space. The success of directed molecular evolution experiment depends on the quality of artificially prepared mutant libraries and the availability of ...
Tianwen Wang, Hu Zhu, Dongzhi Wei
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Directed Molecular Evolution of Cytochrome c Peroxidase
Biochemistry, 2000Cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae was subjected to directed molecular evolution to generate mutants with increased activity against the classical peroxidase substrate guaiacol, thus changing the substrate specificity of CCP from the protein cytochrome c to a small organic molecule.
Iffland A +3 more
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Detection of biological threats. A challenge for directed molecular evolution
Journal of Microbiological Methods, 2004The probe technique originated from early attempts of Anton van Leeuwenhoek to contrast microorganisms under the microscope using plant juices, successful staining of tubercle bacilli with synthetic dyes by Paul Ehrlich and discovery of a stain for differentiation of gram-positive and gram-negative bacteria by Hans Christian Gram.
Valery A, Petrenko, Iryna B, Sorokulova
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Molecular system identification for enzyme directed evolution and design
The Journal of Chemical Physics, 2017The rational design of chemical catalysts requires methods for the measurement of free energy differences in the catalytic mechanism for any given catalyst Hamiltonian. The scope of experimental learning algorithms that can be applied to catalyst design would also be expanded by the availability of such methods.
Xiangying, Guan, Raj, Chakrabarti
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Directed molecular evolution by machine learning and the influence of nonlinear interactions
Journal of Theoretical Biology, 2005Alternative search strategies for the directed evolution of proteins are presented and compared with each other. In particular, two different machine learning strategies based on partial least-squares regression are developed: the first contains only linear terms that represent a given residue's independent contribution to fitness, the second contains ...
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Molecular Evolution Directs Protein Translation Using Unnatural Amino Acids
Current Protocols in Chemical Biology, 2015AbstractUnnatural amino acids have in recent years established their importance in a wide range of fields, from pharmaceuticals to polymer science. Unnatural amino acids can increase the number of chemical groups within proteins and thus expand or enhance biological function.
Vanessa E, Cox, Eric A, Gaucher
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Diverse Plasmid DNA Vectors by Directed Molecular Evolution of Cytomegalovirus Promoters
Human Gene Therapy, 2005Genetic vaccinations, gene therapy, and manufacturing of therapeutic proteins would benefit from promoter sequences that provide improved or prolonged expression levels. The cytomegalovirus (CMV) promoter is one of the most potent promoters known to date, and no previous examples of improved activity of this promoter by sequence mutagenesis have been ...
Anne, Wright +7 more
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[Directed molecular evolution of antibodies].
Journal de la Societe de biologie, 2007Antibodies play a key role in the immune system, are characterized by a homogeneous overall structure and by their ability to interact with an almost unlimited number of compounds. Encoded by a fixed number of genes, they acquire their specificity and affinity of recognition after a succession of genetic recombination and molecular mutation processes ...
Olivier, Dubreuil +4 more
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A novel biotinylated suicide inhibitor for directed molecular evolution of lipolytic enzymes
Bioorganic & Medicinal Chemistry, 2000A bifunctional activity label (8) for directed molecular evolution of lipolytic enzymes has been designed and synthesized. The structure is composed of a 4-nitrophenyl activated phosphonate, that is, a suicide substrate of lipases/esterases, connected to a biotin moiety through a spacer containing a disulfide bridge. The phosphonate (3) was prepared by
H J, Deussen +3 more
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