Results 81 to 90 of about 5,547,631 (284)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Triaxial experimental study on mechanical behavior, acoustic emission characteristics, and energy evolution of coral sand grouting consolidated body under different confining pressures

Case Studies in Construction Materials
This study conducted triaxial compression tests on coral sand grouting consolidated body, incorporating Acoustic Emission (AE) testing to analyze the mechanical properties, AE characteristics, and energy evolution under different strain rates.
Zhenwei Liu, Linjian Ma, Jiajun Deng, Hongya Li, Xudong Chen, Liqun Duan   +5 more
doaj   +1 more source

Topological sensitivity analysis of a time-dependent nonlinear problem

Boundary Value Problems, 2019
We are interested in the optimization of the pipe shape allowing minimization of the dissipated energy in time-dependent Navier–Stokes Darcy flow. The used technique is based on the topological gradient method.
Mohamed Abdelwahed, Azhar Al Salem, Nejmeddine Chorfi, Maatoug Hassine   +3 more
doaj   +1 more source

Resonant Enhancement of Turbulent Energy Dissipation [PDF]

Physical Review Letters, 2010
We periodically modulate a turbulent wind-tunnel flow with an active grid. We find a resonant enhancement of the mean turbulent dissipation rate at a modulation frequency which equals the large-eddy turnover rate. Thus, we find the best frequency to inject energy into a turbulent flow. The resonant response is characterized by the emergence of vortical
Cekli, E.H., Tipton, C.R., Water, van de, W.   +2 more
openaire   +3 more sources

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Relationship between dissipated energy and strain rate in different states.

, 2023
Relationship between dissipated energy and strain rate in different states.
Defeng Hou (16955633), Jianguo Wang (1400488), Wendong Li (3556349), Ximing Jian (16955636), Xianglong Li (1396111), Jiandong Yin (519578), Zhiping Zhang (279585), Wuhu Huang (16955630), Ting Zuo (487849), Yang Yang (45629)   +9 more
core   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Analysis of energy dissipation characteristics in granite under high confining pressure cyclic load

Alexandria Engineering Journal, 2020
With cyclic high in-situ stress, the rock will damage and lead to serious engineering geological disasters. A property of rock failure is the rock damage evolution driven by energy.
Dongjie Yang, Jianhua Hu, Xiaotian Ding
doaj   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source