Results 251 to 260 of about 33,942 (277)
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Neurochemical Research, 1991
The accumulation of [3H]inositol by mechanically dissociated brain cells and cultured skin fibroblasts from fetal mice was examined. Uptake by both tissues was strongly dependent on temperature and the presence of sodium ions. Brain and fibroblast uptake also responded similarly to inhibition by inositol isomers and phloridzin.
B R, Fruen, B R, Lester
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The accumulation of [3H]inositol by mechanically dissociated brain cells and cultured skin fibroblasts from fetal mice was examined. Uptake by both tissues was strongly dependent on temperature and the presence of sodium ions. Brain and fibroblast uptake also responded similarly to inhibition by inositol isomers and phloridzin.
B R, Fruen, B R, Lester
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Cell, 1977
The distribution of microfilament bundles in cells was examined using antibodies to fibroblast myosin and indirect immunofluorescence microscopy. There is no correlation between the presence of bundles of microfilaments and normal growth control. A normal cell line (Balb/c 3T3) cultured on a poorly adhesive substratum showed no microfilament bundles ...
Willingham, M C +4 more
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The distribution of microfilament bundles in cells was examined using antibodies to fibroblast myosin and indirect immunofluorescence microscopy. There is no correlation between the presence of bundles of microfilaments and normal growth control. A normal cell line (Balb/c 3T3) cultured on a poorly adhesive substratum showed no microfilament bundles ...
Willingham, M C +4 more
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Experimental Cell Research, 1967
Abstract A strain of human embryonic fibroblasts (HEB) was compared with the same cells transformed by RSV(HEB-SR) regarding growth curve, DNA synthesis between the time of seeding and the time growth stops, and nuclear overlapping in resting cultures.
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Abstract A strain of human embryonic fibroblasts (HEB) was compared with the same cells transformed by RSV(HEB-SR) regarding growth curve, DNA synthesis between the time of seeding and the time growth stops, and nuclear overlapping in resting cultures.
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Circulation Research, 2014
Pure cardiovascular cell lineages are a requisite for an unbiased and cell-type specific investigation of (patho-) physiological mechanisms involved in heart failure. Moreover, pure heart cells are needed as building blocks for cardiac tissue engineering.
Manuel Kernbach +5 more
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Pure cardiovascular cell lineages are a requisite for an unbiased and cell-type specific investigation of (patho-) physiological mechanisms involved in heart failure. Moreover, pure heart cells are needed as building blocks for cardiac tissue engineering.
Manuel Kernbach +5 more
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ELECTROPHORESIS, 1982
AbstractProtein profiles of skin fibroblasts labelled with [35S]‐methionine from patients with Duchenne muscular dystrophy (DMD) have been compared with those of cells from normal individuals by isoelectric focusing on thin polyacrylamide gels containing denaturing and solubilising agents cast on silanized supports.
Arthur H. M. Burghes +3 more
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AbstractProtein profiles of skin fibroblasts labelled with [35S]‐methionine from patients with Duchenne muscular dystrophy (DMD) have been compared with those of cells from normal individuals by isoelectric focusing on thin polyacrylamide gels containing denaturing and solubilising agents cast on silanized supports.
Arthur H. M. Burghes +3 more
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Breast Cancer Research and Treatment, 2014
Our goal was to establish primary cultures from dissociation of breast tumors in order to provide cellular models that may better recapitulate breast cancer pathogenesis and the metastatic process. Here, we report the characterization of six cellular models derived from the dissociation of primary breast tumor specimens, referred to as "dissociated ...
Katherine, Drews-Elger +14 more
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Our goal was to establish primary cultures from dissociation of breast tumors in order to provide cellular models that may better recapitulate breast cancer pathogenesis and the metastatic process. Here, we report the characterization of six cellular models derived from the dissociation of primary breast tumor specimens, referred to as "dissociated ...
Katherine, Drews-Elger +14 more
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Matrix, 1993
Actin microfilaments of the fibroblasts cultured in a collagen gel were distributed along the inner surface of the entire cell membrane, in either spherical shape at an initial stage of culture or elongated shape at a later stage. The distribution was quite different from that of the fibroblast cultured on a two-dimensional surface, where actin ...
T, Nishiyama +7 more
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Actin microfilaments of the fibroblasts cultured in a collagen gel were distributed along the inner surface of the entire cell membrane, in either spherical shape at an initial stage of culture or elongated shape at a later stage. The distribution was quite different from that of the fibroblast cultured on a two-dimensional surface, where actin ...
T, Nishiyama +7 more
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American Journal of Hypertension, 1992
Skin fibroblasts from newborn spontaneously hypertensive rats (SHR) grow faster in culture than Wistar-Kyoto rat (WKY) cells. Similar results have been described for vascular smooth muscle cells from prehypertensive and adult SHR. This suggests the existence of an intrinsic abnormality in vascular and nonvascular cells of mesodermal origin affecting ...
P, Guicheney +3 more
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Skin fibroblasts from newborn spontaneously hypertensive rats (SHR) grow faster in culture than Wistar-Kyoto rat (WKY) cells. Similar results have been described for vascular smooth muscle cells from prehypertensive and adult SHR. This suggests the existence of an intrinsic abnormality in vascular and nonvascular cells of mesodermal origin affecting ...
P, Guicheney +3 more
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Experimental Cell Research, 1990
The localization of talin and vinculin in chicken embryo fibroblasts (CEF) during transformation was studied by immunoelectron microscopy. CEF cells were infected with a temperature-sensitive mutant of Rous sarcoma virus. After 16 h at 42 degrees C, transformation was induced by incubation at 37 degrees C for different intervals up to 3 h.
R, Brands +3 more
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The localization of talin and vinculin in chicken embryo fibroblasts (CEF) during transformation was studied by immunoelectron microscopy. CEF cells were infected with a temperature-sensitive mutant of Rous sarcoma virus. After 16 h at 42 degrees C, transformation was induced by incubation at 37 degrees C for different intervals up to 3 h.
R, Brands +3 more
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Endokrinologie, 1982
Four fibroblast lines (F1-4) were established from abdominal (F1 and F2) and vulvar (F3 and F4) skin samples obtained from women who underwent delivery. The concentration of dihydrotestosterone (DHT) binding sites was measured along with the maximum velocity for the formation of DHT from testosterone in fibroblasts from each cell line.
H, Ozasa +3 more
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Four fibroblast lines (F1-4) were established from abdominal (F1 and F2) and vulvar (F3 and F4) skin samples obtained from women who underwent delivery. The concentration of dihydrotestosterone (DHT) binding sites was measured along with the maximum velocity for the formation of DHT from testosterone in fibroblasts from each cell line.
H, Ozasa +3 more
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