Results 271 to 280 of about 889,606 (319)

Quantitative investigation on the effect of puromycin on the aggregation of trypsin- and versene-dissociated chick fibroblast cells

Journal of Cell Science, 1967
AbstractEmbryonic chick fibroblast cells were used to test the effects of puromycin on cell adhesion and aggregation. Single cell suspensions were prepared by dissociating the muscle tissues of 5- and 9-day-old chick embryos with either trypsin or versene according to standard cell dissociation procedures.’ Cell aggregation was quantitatively estimated
R B, Kemp, B M, Jones, I, Cunningham, M C, James   +3 more
openaire   +2 more sources

Abstract 2: Automated Dissociation Of Neonatal Hearts And Immunomagnetic Purification Of Cardiomyocytes, Cardiac Fibroblasts And Endothelial Cells

Circulation Research, 2014
Pure cardiovascular cell lineages are a requisite for an unbiased and cell-type specific investigation of (patho-) physiological mechanisms involved in heart failure. Moreover, pure heart cells are needed as building blocks for cardiac tissue engineering.
Manuel Kernbach, Josephine Riesen, Anne Maria Wiencierz, Andreas Bosio, Peter Christalla, Dominik Eckardt   +5 more
openaire   +1 more source

Primary breast tumor-derived cellular models: characterization of tumorigenic, metastatic, and cancer-associated fibroblasts in dissociated tumor (DT) cultures

Breast Cancer Research and Treatment, 2014
Our goal was to establish primary cultures from dissociation of breast tumors in order to provide cellular models that may better recapitulate breast cancer pathogenesis and the metastatic process. Here, we report the characterization of six cellular models derived from the dissociation of primary breast tumor specimens, referred to as "dissociated ...
Katherine, Drews-Elger, Joeli A, Brinkman, Philip, Miller, Sanket H, Shah, J Chuck, Harrell, Thiago G, da Silva, Zheng, Ao, Amy, Schlater, Diana J, Azzam, Kathleen, Diehl, Dafydd, Thomas, Joyce M, Slingerland, Charles M, Perou, Marc E, Lippman, Dorraya, El-Ashry   +14 more
openaire   +2 more sources

Microfilament bundles and cell shape are related to adhesiveness to substratum and are dissociable from growth control in cultured fibroblasts

Cell, 1977
The distribution of microfilament bundles in cells was examined using antibodies to fibroblast myosin and indirect immunofluorescence microscopy. There is no correlation between the presence of bundles of microfilaments and normal growth control. A normal cell line (Balb/c 3T3) cultured on a poorly adhesive substratum showed no microfilament bundles ...
Willingham, M C, Yamada, K M, Yamada, S S, Pouyssegur, J, Pastan, I   +4 more
openaire   +2 more sources

Disintegration of adhesion plaques in chicken embryo fibroblasts upon Rous sarcoma virus-induced transformation: Different dissociation rates for talin and vinculin

Experimental Cell Research, 1990
The localization of talin and vinculin in chicken embryo fibroblasts (CEF) during transformation was studied by immunoelectron microscopy. CEF cells were infected with a temperature-sensitive mutant of Rous sarcoma virus. After 16 h at 42 degrees C, transformation was induced by incubation at 37 degrees C for different intervals up to 3 h.
R, Brands, A, de Boer, C A, Feltkamp, E, Roos   +3 more
openaire   +2 more sources

Dissociation of Actin Microfilament Organization from Acquisition and Maintenance of Elongated Shape of Human Dermal Fibroblasts in Three-Dimensional Collagen Gel

Matrix, 1993
Actin microfilaments of the fibroblasts cultured in a collagen gel were distributed along the inner surface of the entire cell membrane, in either spherical shape at an initial stage of culture or elongated shape at a later stage. The distribution was quite different from that of the fibroblast cultured on a two-dimensional surface, where actin ...
T, Nishiyama, M, Tsunenaga, N, Akutsu, I, Horii, Y, Nakayama, E, Adachi, M, Yamato, T, Hayashi   +7 more
openaire   +2 more sources

Dissociation of the 5 alpha-reductase activity from the specific dihydrotestosterone binding activity in cultured human fibroblasts.

Endokrinologie, 1982
Four fibroblast lines (F1-4) were established from abdominal (F1 and F2) and vulvar (F3 and F4) skin samples obtained from women who underwent delivery. The concentration of dihydrotestosterone (DHT) binding sites was measured along with the maximum velocity for the formation of DHT from testosterone in fibroblasts from each cell line.
H, Ozasa, T, Tominaga, T, Nishimura, T, Takeda   +3 more
openaire   +1 more source

Short exposure to collagenase and coculture with mouse embryonic pancreas improve human dermal fibroblast culture

Biotechnology and applied biochemistry, 2012
S. Pandamooz, A. Hadipour, H. Akhavan‐Niaki, M. Pourghasem, Z. Abedian, A. Ardekani, Monireh Golpour, Z. Hassan, A. Mostafazadeh   +8 more
semanticscholar   +1 more source

Induction of Uncoupling Protein‐1 in Mouse Embryonic Fibroblast‐derived Adipocytes by Retinoic Acid

Obesity, 2010
J. Mercader, A. Palou, M. Bonet
semanticscholar   +1 more source

Integrative oncology: Addressing the global challenges of cancer prevention and treatment

Ca-A Cancer Journal for Clinicians, 2022
Jun J Mao,, Msce, Lorenzo Cohen, Karen M Mustian   +2 more
exaly