Systematic optimization of human pluripotent stem cells media using Design of Experiments. [PDF]
Human pluripotent stem cells (hPSC) are used to study the early stages of human development in vitro and, increasingly due to somatic cell reprogramming, cellular and molecular mechanisms of disease.
Chailangkarn, Thanathom +2 more
core +1 more source
Influence of cell dissociation procedures on the tumorigenicity of Simian Virus 40 transformed fibroblasts [PDF]
Mouse fibroblasts transformed by Simian Virus 40 (SV40) were examined for tumor forming ability in syngeneic BALB/c mice following dissociation from tissue culture dishes by two procedures. A significantly greater in vivo proliferative capacity was observed for cells dissociated by the tryspin-EDTA procedure, with the injected cell dose for tumor ...
Tenforde, T. S. +4 more
openaire +4 more sources
Long-term cultivation of two diploid epithelial cell lines derived from normal rat liver cells [PDF]
To obtain a useful rat liver cell line for in vitro carcinogenesis, two rat diploid epithelial cell lines were established from a 7-day-old male rat by the repeated colonial clone method.
Masuji, Hiroshi +2 more
core +1 more source
Relationship between Fibroblast Growth Factor 23 and Biochemical and Bone Histomorphometric Alterations in a Chronic Kidney Disease Rat Model Undergoing Parathyroidectomy. [PDF]
Phosphate burden in chronic kidney disease (CKD) leads to elevated serum fibroblast factor-23 (FGF-23) levels, secondary hyperparathyroidism and chronic kidney disease-mineral bone disorder (CKD-MBD).
Hung-Wei Liao +7 more
doaj +1 more source
Binding between the neural cell adhesion molecules axonin-1 and Nr- CAM/Bravo is involved in neuron-glia interaction [PDF]
Neural cell adhesion molecules of the immunoglobulin superfamily mediate cellular interactions via homophilic binding to identical molecules and heterophilic binding to other family members or structurally unrelated cell-surface glycoproteins.
Buchstaller, Andrea +5 more
core +4 more sources
Neural crest stem cells undergo multilineage differentiation in developing peripheral nerves to generate endoneurial fibroblasts in addition to Schwann cells [PDF]
Neural crest stem cells (NCSCs) persist in peripheral nerves throughout late gestation but their function is unknown. Current models of nerve development only consider the generation of Schwann cells from neural crest, but the presence of NCSCs raises ...
Anderson, David J. +9 more
core +1 more source
A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes
The mitogenic actions of epidermal growth factor (EGF) were examined in low-density, dissociated cultures of embryonic day 14 mouse striatal primordia, under serum-free defined conditions.
B. Reynolds, W. Tetzlaff, S. Weiss
semanticscholar +1 more source
FGF-2 deficiency does not influence FGF ligand and receptor expression during development of the nigrostriatal system. [PDF]
Secreted proteins of the fibroblast growth factor (FGF) family play important roles during development of various organ systems. A detailed knowledge of their temporal and spatial expression profiles, especially of closely related FGF family members, are
Andreas Ratzka +2 more
doaj +1 more source
To study the interaction of neurons with CNS glial cells, dissociated sympathetic or sensory ganglion cells or fetal retinal cells were plated onto cultures of dissociated optic nerve glial cells of young rats.
M. Schwab, Pica Caroni
semanticscholar +1 more source
Fibroblast Growth Factor Receptor-Mediated Rescue of x-Ephrin B1-Induced Cell Dissociation in XenopusEmbryos [PDF]
The Eph family of receptor tyrosine kinases and their membrane-bound ligands, the ephrins, have been implicated in regulating cell adhesion and migration during development by mediating cell-to-cell signaling events. Genetic evidence suggests that ephrins may transduce signals and become tyrosine phosphorylated during embryogenesis.
L D, Chong +4 more
openaire +2 more sources

