Results 141 to 150 of about 4,651,873 (337)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Using RR Lyrae Stars as High-Precision Distance Indicators in the Infrared

, 2018
RR Lyrae stars have the potential to be high-precision distance indicators, and serve as the anchor for a distance ladder composed of Population II stars. Theoretical pulsation models predict a significant contribution of metallicity on the zero point of
Neeley, JR, Bono, G, Dall'Ora, M, Marconi, M, Braga, VF, Marengo, M, Magurno, D   +6 more
core  

Chicago–Carnegie Hubble Program: A Multiwavelength Search for the Effects of Metallicity on the Cepheid Distance Scale. I. Empirical Evidence

The Astrophysical Journal
Understanding the effects of metallicity on the multiwavelength properties of Cepheids is of continuing importance for their effective use as extragalactic distance indicators and an accurate measure of the Hubble constant, H _0 .
Barry F. Madore, Wendy L. Freedman
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Spatial Complex Network Analysis and Accessibility Indicators: the Case of Municipal Commuting in Sardinia, Italy

, 2011
In this paper a contribution is presented with respect to accessibility indicators modelling for commuters moving through the municipalities of Sardinia, in Italy.
Andrea De Montis, Caschili, Simone, De Montis, Andrea, Alessandro Chessa, Simone Caschili, Chessa, Alessandro, De Montis A   +6 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

The Cepheid Distance Scale: A Novel Method for Obtaining Mean Magnitudes from Single-epoch Observations

The Astronomical Journal
We present a novel technique for mapping single-phase observations of Cepheids in any given band into their time-averaged values, using strong priors on the known interrelations of the multiwavelength widths of Cepheid period–luminosity (PL) relations ...
Barry F. Madore, Wendy L. Freedman, Kayla Owens   +2 more
doaj   +1 more source

Applicability of mitotic figure counting by deep learning: a development and pan‐cancer validation study

FEBS Open Bio, EarlyView.
In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes, Maria X. Isaksen, Frida Julbø, Manohar Pradhan, Andreas Kleppe, Sepp De Raedt, Ole‐Johan Skrede, Turid Torheim, John Arne Nesheim, Hans Martin Mohn, Hanne A. Askautrud, Karolina Cyll, Wanja Kildal, Emma Rewcastle, Melinda Lillesand, Vebjørn Kvikstad, Emiel Janssen, Robert Jones, Odd Terje Brustugun, Bjørn Brennhovd, Erik Skaaheim Haug, Lill‐Tove Rasmussen Busund, Elin Richardsen, Sigve Andersen, Tom Dønnem, Kristina Lindemann, Gunnar Kristensen, Neil A. Shepherd, Marco Novelli, Knut Liestøl, David Kerr, Håvard E. Danielsen, Tarjei S. Hveem   +32 more
wiley   +1 more source

The Milky Way as Seen by Classical Cepheids. I. Distances Based on Mid-infrared Photometry

The Astrophysical Journal Supplement Series
Classical Cepheids are the archetype of the standard candle, thanks to the period–luminosity relation, which allows us to measure their intrinsic brightness.
Dorota M. Skowron, Ronald Drimmel, Shourya Khanna, Alessandro Spagna, Eloisa Poggio, Pau Ramos   +5 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source