Results 1 to 10 of about 270,794 (145)

Development of a 96-Well Plate Dithiothreitol Method for the Colorimetric Determination of Nickel Ions in Water Samples [PDF]

open access: yesSensors
A new, simple, and accurate assay was developed for the colorimetric determination of nickel ions in 96-well plates. The proposed method utilized the color change immediately visible even to the naked eye during the reaction of Ni(II) ions with ...
George N. Nikas   +2 more
doaj   +2 more sources

Application and concentration selection of dithiothreitol for correcting false elevation of D-dimer [PDF]

open access: yesPractical Laboratory Medicine
Background: Immunological methods for D-dimer detection are prone to interference, leading to falsely elevated values. This study evaluates the optimal concentration and corrective efficacy of dithiothreitol (DTT) in addressing false D-dimer elevations ...
Jia-Wei Zeng, Guang-Hao Song, Mao Xia
doaj   +2 more sources

Immobilization of Firefly Bioluminescent System: Development and Application of Reagents

open access: yesBiosensors, 2022
The present study describes the method of preparing reagents containing firefly luciferase (FLuc) and its substrate, D-luciferin, immobilized into gelatin gel separately or together.
Elena N. Esimbekova   +2 more
doaj   +1 more source

Long-term storage protocol of reagent red blood cells treated with 0.01M dithiothreitol (DTT) for pre-transfusion testing of patients receiving anti-CD38 therapy, daratumumab

open access: yesHematology, 2023
Objective: Use red blood cell stabilizer to store the antibody screening and antibody identification reagent red blood cells (RBCs) treated with 0.01 mol/L DTT and investigate its value in the pre-transfusion examinations of patients treated with ...
Yuyuan Li   +8 more
doaj   +1 more source

Technique for Determining the Viability of Acanthamoeba Cysts Treated with a Cysticidal Agent Based on Membrane Integrity

open access: yesInternational Journal of Technology, 2021
This study presents a straightforward and reliable method for determining the viability of Acanthamoeba cysts. A standard method for determining Acanthamoeba cyst viability in an in vitro cytotoxicity analysis is required to ensure that the double ...
Nor Farah Azwani Che Mohamad   +7 more
doaj   +1 more source

Contribution of Physical and Chemical Properties to Dithiothreitol-Measured Oxidative Potentials of Atmospheric Aerosol Particles at Urban and Rural Sites in Japan

open access: yesAtmosphere, 2022
Dithiothreitol-measured oxidative potential (OPDTT) can chemically quantify the adverse health effects of atmospheric aerosols. Some chemical species are characterized with DTT activities, and the particle diameter and surface area control DTT ...
Kazuki Kurihara   +6 more
doaj   +1 more source

An Overview of the Automated and On-Line Systems to Assess the Oxidative Potential of Particulate Matter

open access: yesAtmosphere, 2023
Recent years have seen a significant increase in the scientific literature related to various methods for analyzing oxidative potential (OP) of atmospheric particulate matter (PM).
Alessandro Carlino   +4 more
doaj   +1 more source

Investigation of the Efficacy of Dithiothreitol and Glutathione on In Vitro Fertilization of Cryopreserved Large White Boar Semen

open access: yesAnimals, 2022
The objectives of this study were to evaluate the properties of sperm motility and morphology under induced oxidative stress, compare the antioxidant capacity of dithiothreitol (DTT) and glutathione (GSH) following the cryopreservation of Large White ...
Mahlatsana Ramaesela Ledwaba   +4 more
doaj   +1 more source

Dithiothreitol causes toxicity in C. elegans by modulating the methionine–homocysteine cycle

open access: yeseLife, 2022
The redox reagent dithiothreitol (DTT) causes stress in the endoplasmic reticulum (ER) by disrupting its oxidative protein folding environment, which results in the accumulation and misfolding of the newly synthesized proteins. DTT may potentially impact
Gokul G, Jogender Singh
doaj   +1 more source

Gluten Conformation at Different Temperatures and Additive Treatments

open access: yesFoods, 2022
The effect of temperature (25, 45, and 65 °C) on the gluten secondary structure was investigated by using Fourier transform infrared (FTIR) spectroscopy and modulation of disulfide and hydrogen bonds contributions (100 ppm ascorbic acid (AA), 0.6 ...
Pavalee Chompoorat   +3 more
doaj   +1 more source

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