Toxicity of Dithiothreitol (DTT) to Drosophila melanogaster
Toxicology Reports, 2021 The thiol-containing compound Dithiothreitol (DTT) has been shown to be toxic to cultured cells by inducing the generation of reactive oxygen species that ultimately cause cell death.
Yiwen Wang +5 more
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Sphingomyelinase activity at pH 7.4 in human brain and a comparison to activity at pH 5.0
Journal of Lipid Research, 1976 A hitherto undescribed sphingomyelinase (sph'ase 7.4) of human brain has been studied in crude and partially purified (3- to 4- fold) extracts of grey matter, and compared to the known sphingomyelinase with an acid pH optimum (sph'ase 5.0).
B G Rao, Dr M W Spence
doaj +1 more source
Resolving crossmatch incompatibility due to daratumumab in multiple myeloma
Global Journal of Transfusion Medicine, 2020 Daratumumab (DARA) is an anti-CD38 monoclonal antibody which has promising results in relapsed and refractory multiple myeloma. DARA interferes with blood compatibility testing by causing panagglutination in the indirect antiglobulin test.
S Anuragaa +2 more
doaj +1 more source
Zhongguo shuxue zazhi, 2022 Objective To explore the challenging blood cross-matching and resolution for multiple myeloma (MM) patients in different disease stages. Methods For a patient who was first diagnosed as MM and scheduled for blood transfusion, his blood was cross matched ...
Ying LU +6 more
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The Effects of Dithiothreitol on DNA [PDF]
Sensors, 2017 With the novel possibilities for detecting molecules of interest with extreme sensitivity also comes the risk of encountering hitherto negligible sources of error. In life science, such sources of error might be the broad variety of additives such as dithiothreitol (DTT) used to preserve enzyme stability during in vitro reactions.
Søren Fjelstrup +8 more
openaire +3 more sources
A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment [PDF]
Acta AmazonicaMany tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies.
Érica MANGARAVITE +5 more
doaj +2 more sources
Properties and stabilization of an extracellular α-glucosidase from the extremely thermophilic archaebacteria Thermococcus strain AN 1: enzyme activity at 130°C [PDF]
, 1996 An extracellular α-glucosidase from the thermophilic archaebacterium Thermococcus strain AN1 was purified 875-fold in five steps (Hiload Q-Sepharose, phenyl Sepharose, HPHT-hydroxyapatite, gel filtration and Mono Q chromatography) with a yield of 4%.
Daniel, Roy M. +2 more
core +2 more sources
NsrR from Streptomyces coelicolor is a nitric oxide-sensing [4Fe-4S] cluster protein with a specialized regulatory function [PDF]
, 2015 The Rrf2 family transcription factor NsrR controls expression of genes in a wide range of bacteria in response to nitric oxide (NO). The precise form of the NO-sensing module of NsrR is the subject of controversy because NsrR proteins containing either ...
Al-Bassam, Mahmoud +12 more
core +1 more source
Global Journal of Transfusion Medicine, 2021 Daratumumab (DARA) may interfere with immunohematology tests by binding to endogenous CD38 present on the surface of the red blood cells. DARA was Food and Drug Administration approved for refractory multiple myeloma patients, but now its application is ...
Abhaykumar Malind Gupta +3 more
doaj +1 more source
DNA intercalation and cleavage of an antitumor antibiotic dynemicin that contains anthracycline and enediyne cores [PDF]
, 1990 Dynemicin is a hybrid containing anthraquinone and enediyne cores, which contribute to binding and cleavage of DNA, respectively. DNA strand scission by the antitumor antibiotic is significantly enhanced by the addition of NADPH or thiol compounds.
Konishi, Masataka +3 more
core +1 more source