Results 1 to 10 of about 60,289 (232)
Archaeal DNA Repair Mechanisms [PDF]
Biomolecules, 2020 Archaea often thrive in environmental extremes, enduring levels of heat, pressure, salinity, pH, and radiation that prove intolerable to most life. Many environmental extremes raise the propensity for DNA damaging events and thus, impact DNA stability ...
Craig J. Marshall, Thomas J. Santangelo
doaj +3 more sources
Characterization of archaeal promoters using explainable and web-based CNN model [PDF]
BMC GenomicsBackground Archaea, the third realm of life, shows unique genetic and metabolic adaptations that enable its survival in harsh environments. Despite its biotechnological and evolutionary importance, limited research has been done on archaeal promoter ...
Muhammad Shujaat, Shi-Qing Mao
doaj +2 more sources
The influence of primer choice on archaeal phylogenetic analyses based on 16S rRNA gene PCR
Brazilian Journal of Biology, 2021 Polymerase chain reaction (PCR) assays targeting 16S rRNA genes followed by DNA sequencing are still important tools to characterize microbial communities present in environmental samples.
A. Belmok +4 more
doaj +1 more source
Data on the optimization of an archaea-specific probe-based qPCR assay
Data in Brief, 2020 Estimation of archaeal numbers by use of fluorescent DNA binding dyes is challenging, because primers targeting the archaeal 16SrRNA genes readily also bind to bacterial 16S rRNA gene sequences, especially when the relative abundance of bacteria is ...
Malin Bomberg, Hanna Miettinen
doaj +1 more source
Archaeal DNA-import apparatus is homologous to bacterial conjugation machinery
Nature Communications, 2023 Bacteria can exchange DNA through extracellular appendages (‘mating pili’) in a process known as conjugation. Here, Beltran et al. determine atomic structures by cryo-electron microscopy of a bacterial conjugative pilus and two archaeal pili, showing ...
Leticia C. Beltran +11 more
doaj +1 more source
PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]
, 2012 As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Ameet J. Pinto +2 more
core +7 more sources
The replication machinery of LUCA: common origin of DNA replication and transcription
BMC Biology, 2020 Origin of DNA replication is an enigma because the replicative DNA polymerases (DNAPs) are not homologous among the three domains of life, Bacteria, Archaea, and Eukarya. The homology between the archaeal replicative DNAP (PolD) and the large subunits of
Eugene V. Koonin +3 more
doaj +1 more source
Crystal structures of ternary complexes of archaeal B-family DNA polymerases. [PDF]
PLoS ONE, 2017 Archaeal B-family polymerases drive biotechnology by accepting a wide substrate range of chemically modified nucleotides. By now no structural data for archaeal B-family DNA polymerases in a closed, ternary complex are available, which would be the basis
Heike M Kropp +4 more
doaj +1 more source
Frontiers in Microbiology, 2021 Histone proteins compact and organize DNA resulting in a dynamic chromatin architecture impacting DNA accessibility and ultimately gene expression. Eukaryotic chromatin landscapes are structured through histone protein variants, epigenetic marks, the ...
Travis J. Sanders +8 more
doaj +1 more source
Frontiers in Microbiology, 2020 Archaeal species encode a variety of distinct lineage-specific chromosomal proteins. We have previously shown that in Thermococcus kodakarensis, histone, Alba, and TrmBL2 play distinct roles in chromosome organization.
Hugo Maruyama +7 more
doaj +1 more source