Results 121 to 130 of about 1,269,047 (286)
TTT and R2TP chaperone complexes are required for the assembly and activation of mTORC1. WAC directly interacts with components of TTT, R2TP, and mTORC1, and these interactions are affected by the availability of glucose and glutamine, correlating with changes in mTORC1 activity.
Sofía Cabezudo +11 more
wiley +1 more source
Intravesicle Isothermal DNA Replication
Background Bacterial and viral DNA replication was previously reconstituted in vitro from component parts 1234. Significant advances in building minimal cell-like structures also have been made recently 567.
Ross Lindsey A +4 more
doaj +1 more source
Recognition of a tandem lesion by DNA bacterial formamidopyrimidine glycosylases explored combining molecular dynamics and machine learning. [PDF]
Bignon E +5 more
europepmc +1 more source
Microbial profile of the appendix niche in acute appendicitis: a novel sampling approach
This study utilized a novel sampling method, ERAT (i.e. endoscopic retrograde appendicitis treatment)‐guided lumen aspiration, to obtain samples from the appendix, and shotgun metagenomic sequencing was performed for in situ characterization of the appendix microbiome in patients with acute appendicitis.
Huimin Ma +10 more
wiley +1 more source
Multiple gene sequence analysis using genes of the bacterial DNA repair pathway
The ability to recognize and repair abnormal DNA structures is common to all forms of life. Physiological studies and genomic sequencing of a variety of bacterial species have identified an incredible diversity of DNA repair pathways.
Miguel Rotelok Neto +6 more
doaj +1 more source
Structural and functional characterization of chitinase from carnivorous plant Drosera adelae
A class I chitinase from the carnivorous plant Drosera adelae was expressed and purified using a yeast system, revealing high enzymatic activity. Structural analyses of the catalytic and chitin‐binding domains identified key tyrosine residues involved in substrate binding, offering insights into the enzyme's adaptation for insect digestion.
Kazunari Yoneda +7 more
wiley +1 more source
Report on the 2nd MObility for Vesicle research in Europe (MOVE) symposium—2024
The 2nd MObility for Vesicle research in Europe (MOVE) Symposium in Belgrade brought over 280 attendees from 28 countries to advance extracellular vesicle (EV) research. Featuring keynotes, presentations, and industry sessions, it covered EV biogenesis, biomarkers, therapies, and manufacturing.
Dorival Mendes Rodrigues‐Junior +5 more
wiley +1 more source
Application of PCR-DGGE in determining food origin : cases studies of fish and fruits [PDF]
The determination of geographical origin is a demand of the traceability system of import-export food products. For this purpose, molecular techniques employing 16S, 26S rDNA profiles generated by PCR-DGGE (polymerase chain reaction - denaturing gradient
Condur, Ana +5 more
core
Sarcosine oxidase from Bacillus sp. (SoxB) recognizes l‐proline (l‐Pro), d‐proline (d‐Pro), and l‐thioproline (l‐Tpr) as minor substrates. In this study, we measured their absorption spectra, determined the crystal structures of their enzyme complexes, and performed site‐directed mutagenesis of key residues.
Yuqi Zhang +5 more
wiley +1 more source
Isolating Bacterial DNA from Goat Milk and Its Products: Comparative Analysis
Despite its high demand in molecular diagnostics, the bacterial DNA extraction from dairy products remains an understudied area. The effectiveness of different DNA extraction methods for detecting microorganisms in food matrices needs a comprehensive ...
Koval Daria +3 more
doaj +1 more source

