Results 101 to 110 of about 1,303,684 (303)

DNA extraction: High yield is not synonymous with high diversity

Bioingeniøren, 2017
The quality, amount, and, in the case of polymicrobial samples, diversity of isolated DNA presupposes the precision of downstream application results. As part of a larger study on colorectal cancer, the aim of this work was to identify the DNA extraction
John Christopher Noone, Vahid Bemanian, Hege Smith Tunsjø   +2 more
doaj  

Oral bacterial DNA findings in pericardial fluid

Journal of Oral Microbiology, 2014
Background: We recently reported that large amounts of oral bacterial DNA can be found in thrombus aspirates of myocardial infarction patients. Some case reports describe bacterial findings in pericardial fluid, mostly done with conventional culturing ...
Anne-Mari Louhelainen, Joonas Aho, Sari Tuomisto, Janne Aittoniemi, Risto Vuento, Pekka J. Karhunen, Tanja Pessi   +6 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

DnaG Primase—A Target for the Development of Novel Antibacterial Agents

Antibiotics, 2018
The bacterial primase—an essential component in the replisome—is a promising but underexploited target for novel antibiotic drugs. Bacterial primases have a markedly different structure than the human primase.
Stefan Ilic, Shira Cohen, Meenakshi Singh, Benjamin Tam, Adi Dayan, Barak Akabayov   +5 more
doaj   +1 more source

Phosphorothioate-DNA bacterial diet reduces the ROS levels in C. elegans while improving locomotion and longevity. [PDF]

Commun Biol, 2021
Huang Q, Li R, Yi T, Cong F, Wang D, Deng Z, Zhao YL.   +6 more
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Large-scale capsid-mediated mobilisation of bacterial genomic DNA in the gut microbiome

Nature Communications
Transducing bacteriophage and gene transfer agents (GTAs) are constrained by the structural limits of their capsids, which determine the maximum length of host DNA they can package.
Tatiana Borodovich, Colin Buttimer, Jason S. Wilson, Pavol Bardy, Muireann Smith, Conor Hill, Ekaterina V. Khokhlova, Matthew Harte, Bianca Govi, Paul C. M. Fogg, Colin Hill, Andrey N. Shkoporov   +11 more
doaj   +1 more source

Author Correction: Phosphorothioate-DNA bacterial diet reduces the ROS levels in C. elegans while improving locomotion and longevity. [PDF]

Commun Biol, 2021
Huang Q, Li R, Yi T, Cong F, Wang D, Deng Z, Zhao YL.   +6 more
europepmc   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Recognition of a tandem lesion by DNA bacterial formamidopyrimidine glycosylases explored combining molecular dynamics and machine learning. [PDF]

Comput Struct Biotechnol J, 2021
Bignon E, Gillet N, Chan CH, Jiang T, Monari A, Dumont E.   +5 more
europepmc   +1 more source