Results 121 to 130 of about 854,281 (317)

Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.)

Parasites & Vectors, 2011
Background Bacteria associated with insects can have a substantial impact on the biology and life cycle of their host. The checkerboard DNA-DNA hybridization technique is a semi-quantitative technique that has been previously employed in odontology to ...
Gaio Analiz de Oliveira, Rodrigues Rivea CC, do Nascimento Cássio, Secundino Nagila FC, Lemos Francisco JA, Pimenta Paulo FP, Monesi Nadia   +6 more
doaj   +1 more source

Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?

PLoS ONE, 2020
BACKGROUND:High-throughput sequencing techniques are used to analyse the diversity of the respiratory microbiota in health and disease. Although extensive data are available regarding bacterial respiratory microbiota, its fungal component remains poorly ...
Cécile Angebault, Mathilde Payen, Paul-Louis Woerther, Christophe Rodriguez, Françoise Botterel   +4 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Microbial DNA fingerprinting of human fingerprints: dynamic colonization of fingertip microflora challenges human host inferences for forensic purposes [PDF]

, 2009
Human fingertip microflora is transferred to touched objects and may provide forensically relevant information on individual hosts, such as on geographic origins, if endogenous microbial skin species/strains would be retrievable from physical ...
Wamel, W.J.B. (Willem) van, Belkum, A., van, Belkum, Alex, Tims, S. (Sebastian), Sebastian Tims, Willem van Wamel, Belkum, A.F. (Alex) van, Kayser, M.H. (Manfred), Tims, S., Endtz, H.P., Endtz, Hubert, Tims, S (Sebastian), Endtz, H.P. (Hubert), Kayser, M., Manfred Kayser, van Belkum, A., van Wamel, Willem, Wamel, W., van, van Wamel, W., Hubert P. Endtz, van Wamel, Willem; id_orcid, Kayser, Manfred, Alex van Belkum   +22 more
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Intravesicle Isothermal DNA Replication

BMC Research Notes, 2011
Background Bacterial and viral DNA replication was previously reconstituted in vitro from component parts 1234. Significant advances in building minimal cell-like structures also have been made recently 567.
Ross Lindsey A, Del Bianco Cristina, Torino Domenica, Ong Jennifer L, Mansy Sheref S   +4 more
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Impact of feeding by Arenicola marina (L.) and ageing of faecal material on fatty acid distribution and bacterial community structure in marine sediments: An experimental approach [PDF]

, 2006
The fate of ingested eukaryotic photoautotrophic fatty acids during gut transit in the lugwormArenicolamarina (L.) and the influence of A. marina's faeces on the evolution of fatty acid distribution and bacterial community structure in superficial ...
Caradec, S, Cuny, P, Nerini, David, Nerini, D, Gilbert, F, Cuny, Philippe, Caradec, Sarah, Gilbert, Franck, Pancost, Richard, Grossi, Vincent, Grossi, V, Pancost, RD   +11 more
core   +1 more source

Isolating Bacterial DNA from Goat Milk and Its Products: Comparative Analysis

Техника и технология пищевых производств
Despite its high demand in molecular diagnostics, the bacterial DNA extraction from dairy products remains an understudied area. The effectiveness of different DNA extraction methods for detecting microorganisms in food matrices needs a comprehensive ...
Koval Daria, Khan Aleksej, Lazareva Ekaterina, Fomenko Oleg   +3 more
doaj   +1 more source

Multiple gene sequence analysis using genes of the bacterial DNA repair pathway

Brazilian Archives of Biology and Technology, 2015
The ability to recognize and repair abnormal DNA structures is common to all forms of life. Physiological studies and genomic sequencing of a variety of bacterial species have identified an incredible diversity of DNA repair pathways.
Miguel Rotelok Neto, Carolina Weigert Galvão, Leonardo Magalhães Cruz, Dieval Guizelini, Leilane Caline Silva, Jarem Raul Garcia, Rafael Mazer Etto   +6 more
doaj   +1 more source