Results 11 to 20 of about 1,269,047 (286)
Optimizing bacterial DNA extraction in urine.
PLoS ONE, 2019 Urine is an acceptable, non-invasive sample for investigating the human urogenital microbiota and for the diagnosis of sexually transmitted infections.
Matthew M Munch +6 more
doaj +5 more sources
Is human DNA enough? - Potential for bacterial DNA [PDF]
Frontiers in Genetics, 2013 Human identification has played an important role in forensic science for the past two decades and it will continue to do so. However, there are certain types of traces, for example, low quality and low quantity of DNA, often associated with violent crimes, which cannot always be satisfactorily exploited by current techniques. So what is next?
Sarah Louise Leake
doaj +4 more sources
Molecular Microbiology, 2001 DNA ligases join breaks in the phosphodiester backbone of DNA molecules and are used in many essential reactions within the cell. All DNA ligases follow the same reaction mechanism, but they may use either ATP or NAD+ as a cofactor. All Bacteria (eubacteria) contain NAD+‐dependent DNA ligases, and the uniqueness of these enzymes to Bacteria makes them ...
Wilkinson, A, Day, J, Bowater, R
openaire +4 more sources
Ribonucleotides in bacterial DNA [PDF]
Critical Reviews in Biochemistry and Molecular Biology, 2014 In all living cells, DNA is the storage medium for genetic information. Being quite stable, DNA is well-suited for its role in storage and propagation of information, but RNA is also covalently included in DNA through various mechanisms. Recent studies also demonstrate useful aspects of including ribonucleotides in the genome during repair.
Justin R. Randall +3 more
openaire +3 more sources
Bacterial DNA in the diagnosis of spontaneous bacterial peritonitis [PDF]
Alimentary Pharmacology & Therapeutics, 2010 Despite inoculation into blood culture bottles, ascitic fluid culture is negative in 50% of cases of spontaneous bacterial peritonitis (SBP).To determine whether 16S rDNA gene detection by real-time polymerase chain reaction (PCR) and sequencing increases the efficacy of culture in microbiological diagnosis of spontaneous bacterial peritonitis.We ...
Michel Montemayor +15 more
openaire +4 more sources
Organization of DNA in a bacterial nucleoid [PDF]
BMC Microbiology, 2016 It is unclear how DNA is packaged in a bacterial cell in the absence of nucleosomes. To investigate the initial level of DNA condensation in bacterial nucleoid we used in vivo DNA digestion coupled with high-throughput sequencing of the digestion-resistant fragments. To this end, we transformed E.
Tolstorukov, Michael Y. +3 more
openaire +4 more sources
Presence of multiple bacterial markers in clinical samples might be useful for presumptive diagnosis of infection in cirrhotic patients with culture-negative reports [PDF]
, 2016 Bacterial infections in cirrhotic patients with ascites are associated with a severe prognosis and an increased risk of death. The microbiological standard tests for the diagnosis of suspected infection, based on culture test of blood and ascitic fluid ...
Boaretti, M +4 more
core +1 more source
Frontiers in Microbiology, 2019 Acinetobacter baumannii is a multidrug-resistant nosocomial opportunistic pathogen that is becoming a major health threat worldwide. In this study, we have focused on the A.
Morgane Roussin +7 more
doaj +1 more source
Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]
, 2019 IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
Ackerman, A Lenore +8 more
core +3 more sources
Frontiers in Microbiology, 2018 Cold-active bacteria of the genus Polaromonas (class Betaproteobacteria) are important components of glacial microbiomes. In this study, extrachromosomal replicons of 26 psychrotolerant Polaromonas strains, isolated from Arctic and Antarctic glaciers ...
Anna Ciok +8 more
doaj +1 more source