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Preparation of Bacterial Plasmid DNA
Current Protocols in Neuroscience, 1998AbstractThe protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is an alkaline lysis miniprep suitable for screening a moderate number of bacterial colonies by restriction endonuclease cleavage and agarose gel electrophoresis.
J, Engebrecht, J S, Heilig, R, Brent
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Dodecylamine in the isolation of bacterial DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971Abstract Lysis of enterobacteria by lysozyme and detergent was greatly accelerated by n-dodecylamine. Nicking of supercoiled DNA was not detected.
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Bacterial Dna in Mixed Cholesterol Gallstones
American Journal of Gastroenterology, 1999Numerous investigators have proposed a role for bacteria in biliary lithogenesis. We hypothesized that bacterial DNA is present in gallstones, and that categorical differences exist between gallstone type and the frequency of bacterial sequences.Polymerase chain reaction (PCR) was used to amplify bacterial 16S rRNA and uidA (encoding Escherichia coli ...
Haigh, WG, Tarr, PI, Lee, DK, Lee, SP
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Comparing Bacterial DNA Microarray Fingerprints
Statistical Applications in Genetics and Molecular Biology, 2005Epidemiologic and forensic investigations often require assays to detect subtle genetic differences between closely related microorganisms. Typically, gel electrophoresis is used to compare randomly amplified DNA fragments between microbial samples, where the patterns of DNA fragment sizes are viewed as genotype fingerprints.
Willse, Alan +5 more
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Separation of Choanoflagellate and Bacterial Genomic DNA
Cold Spring Harbor Protocols, 2009INTRODUCTIONChoanoflagellates are heterotrophic nanoflagellates: small, colorless protozoa that are present in marine and freshwater environments as well as in hydrated soils. Because they are the closest living relatives of the metazoa, the study of their cell biology and genomes promises to provide new insights into metazoan ancestry and origins ...
Nicole, King +4 more
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DNA PROCESSING REACTIONS IN BACTERIAL CONJUGATION
Annual Review of Biochemistry, 1995Bacterial conjugation is an important source of genetic plasticity. The initiation complex for conjugative transfer of transmissible plasmids—the relaxosome—is a specific DNA-protein structure that has been isolated from cells and reconstituted from purified components in vitro.
E, Lanka, B M, Wilkins
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Bacterial DNA Methylation and Methylomes
2016Formation of C5-methylcytosine, N4-methylcytosine, and N6-methyladenine in bacterial genomes is postreplicative and involves transfer of a methyl group from S-adenosyl-methionine to a base embedded in a specific DNA sequence context. Most bacterial DNA methyltransferases belong to restriction-modification systems; in addition, "solitary" or "orphan ...
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Persistence of bacterial DNA in orthopedic infections
Diagnostic Microbiology and Infectious Disease, 2018Polymerase chain reaction (PCR) has been proposed as a method to identify bacteria in clinical samples because it is more sensitive than culture techniques and can produce results rapidly. However, PCR can detect DNA from dead cells and thus cannot distinguish between live and dead cells in a tissue sample.
Heidi B, Kaplan +4 more
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DNA transfer and DNA synthesis during bacterial conjugation
Zeitschrift f�r Vererbungslehre, 1966Mutant strains ofEscherichia coli, which were thermosensitive with respect to DNA replication, were used for conjugation experiments at 37°C and 42°C. Inhibition of DNA synthesis in the donor strain has no influence on the yield of recombinats. Inhibition of DNA synthesis in the recipient strain is accompanied by a complete loss of recombinant ...
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Bacterial topoisomerases and the control of DNA supercoiling
Trends in Genetics, 1990DNA in bacterial cells is under negative superhelical tension, a feature that facilitates many of the activities of DNA. Supercoiling is introduced enzymatically by DNA gyrase, and the accumulation of excessively high levels is prevented by the relaxing activity of DNA topoisomerase I.
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