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Bacterial DNA Methylation and Methylomes
2016Formation of C5-methylcytosine, N4-methylcytosine, and N6-methyladenine in bacterial genomes is postreplicative and involves transfer of a methyl group from S-adenosyl-methionine to a base embedded in a specific DNA sequence context. Most bacterial DNA methyltransferases belong to restriction-modification systems; in addition, "solitary" or "orphan ...
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DNA transfer and DNA synthesis during bacterial conjugation
Zeitschrift f�r Vererbungslehre, 1966Mutant strains ofEscherichia coli, which were thermosensitive with respect to DNA replication, were used for conjugation experiments at 37°C and 42°C. Inhibition of DNA synthesis in the donor strain has no influence on the yield of recombinats. Inhibition of DNA synthesis in the recipient strain is accompanied by a complete loss of recombinant ...
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1974
Publisher Summary This chapter discusses the isolation and physicochemical properties. Methods for isolating the bacterial polymerase share many common features. Cells are lysed by the vigorous disruptions of sonication or mechanical shear or by gentler lysozyme treatments.
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Publisher Summary This chapter discusses the isolation and physicochemical properties. Methods for isolating the bacterial polymerase share many common features. Cells are lysed by the vigorous disruptions of sonication or mechanical shear or by gentler lysozyme treatments.
openaire +1 more source