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Phage-encoded homing endonucleases attenuate bacterial immunity
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Preparing Bacterial Genomic DNA
Cold Spring Harbor Protocols, 2022We describe two alternative procedures for purifying bacterial chromosomal DNA. The first procedure incorporates the use of a commercial kit based on silica membrane technology. This approach relies on the selective binding of DNA to a silica-based column in the presence of chaotropic salts (guanidine salts).
Nara Figueroa-Bossi +2 more
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Preparation of Bacterial Plasmid DNA
Current Protocols in Neuroscience, 1998AbstractThe protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is an alkaline lysis miniprep suitable for screening a moderate number of bacterial colonies by restriction endonuclease cleavage and agarose gel electrophoresis.
JoAnne Engebrecht +2 more
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DNA transfer in bacterial conjugation
Journal of Molecular Biology, 1966Chromosome transfer from Hfr to F − cells was analyzed by labeling the DNA of the Hfr cells with [ 3 H]thymine prior to mating, and/or by labeling any DNA made during mating. The F − cells were either starved of adenine or heavily irradiated with ultraviolet light, with the aim of preventing them from synthesizing DNA.
Lucien G. Caro +3 more
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Chloramphenicol damages bacterial DNA
Biochemical and Biophysical Research Communications, 1977L(+)-threo-chloramphenicol induces reversion of His−Salmonella typhimurium strains TA100 and TA1535 in the conventional Ames' assay without microsomal activation. Any mutagenicity of D(−)-threo-chloramphenicol was masked by toxicity. Similarly, a sensitive fluctuation test showed mutagenesis with L(+)-threo-chloramphenicol at concentrations of 0.5 μM ...
Karl B. Freeman +3 more
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Role of DNA in Bacterial Aggregation
Current Microbiology, 2008The role of DNA in bacterial aggregation was determined using various types of DNA and Escherichia coli, a good model for investigating the correlation between added polymer and bacterial aggregation and adsorption of polymer to bacterial surfaces. The results of the aggregation assay suggest that extracellular DNA indeed increased the aggregation ...
Yi-Ran Yang +5 more
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Arrest of Bacterial DNA Replication
Annual Review of Microbiology, 1992The chromosomes of both gram-positive and gram-negative bacteria contain sites that arrest the progression of DNA replication forks. These replication-arrest sites limit the end of the replication cycle to a particular region of the chromosome, called the terminus region.
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Bacterial DNA in Clarkia fossils
Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 1991For the growing number of investigators who study ancient DNA, the most stimulating paper published during 1990 reported the determination of a chloroplast DNA sequence from a plant compression fossil found in a Miocene deposit at Clarkia, Idaho (Golenberg et al. 1990). During August 1990, S.P.
Arend Sidow +2 more
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Bacterial Dna in Mixed Cholesterol Gallstones
American Journal of Gastroenterology, 1999Numerous investigators have proposed a role for bacteria in biliary lithogenesis. We hypothesized that bacterial DNA is present in gallstones, and that categorical differences exist between gallstone type and the frequency of bacterial sequences.Polymerase chain reaction (PCR) was used to amplify bacterial 16S rRNA and uidA (encoding Escherichia coli ...
Haigh, WG, Tarr, PI, Lee, DK, Lee, SP
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