Results 81 to 90 of about 428,487 (304)

Nucleic acids and protein synthesis in cancer cell mitochondria. I. Nucleic acids in rat hepatoma mitochondria [PDF]

, 1967
The contents of nucleic acids in rat liver and hepatoma mitochondria and the physico-chemical properties on DNA's isolated from these mitochondria were comparatively investigated. The results are briefly summarized as follows. 1.
Inaba, Kozo
core   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Similar gene estimates from circular and linear standards in quantitative PCR analyses using the prokaryotic 16S rRNA gene as a model. [PDF]

PLoS ONE, 2012
Quantitative PCR (qPCR) is one of the most widely used tools for quantifying absolute numbers of microbial gene copies in test samples. A recent publication showed that circular plasmid DNA standards grossly overestimated numbers of a target gene by as ...
Athenia L Oldham, Kathleen E Duncan
doaj   +1 more source

Macroscopic loop formation in circular DNA denaturation

, 2012
The statistical mechanics of DNA denaturation under fixed linking number is qualitatively different from that of the unconstrained DNA. Quantitatively different melting scenarios are reached from two alternative assumptions, namely, that the denatured ...
Alkan Kabakçıoğlu, Amir Bar, B. Alberts, David Mukamel   +3 more
core   +3 more sources

Circular Dichroism of DNA Frayed Wires [PDF]

Biophysical Journal, 1998
Ultraviolet circular dichroism spectra are reported for the oligonucleotide d(A15G15) in aqueous solutions containing 5 mM MgCl2 at several temperatures and in the presence of partially complementary oligonucleotides. Oligonucleotides with several consecutive terminal guanine residues self-associate to form aggregates, called frayed wires, that consist
Protozanova, Ekaterina, Macgregor, Robert B.   +1 more
openaire   +2 more sources

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Detection of HBV Covalently Closed Circular DNA

Viruses, 2017
Chronic hepatitis B virus (HBV) infection affects approximately 240 million people worldwide and remains a serious public health concern because its complete cure is impossible with current treatments.
Xiaoling Li, Jinghua Zhao, Quan Yuan, Ningshao Xia   +3 more
doaj   +1 more source

Electrostatic contribution to DNA condensation - application of 'energy minimization' in a simple model in strong Coulomb coupling regime

, 2011
Bending of DNA from a straight rod to a circular form in presence of any of the mono-, di-, tri- or tetravalent counterions has been simulated in strong Coulomb coupling environment employing a previously developed energy minimization simulation ...
Arup K Mukherjee, Cherstvy A G, Messina R, Mukherjee A K, Mukherjee A K, Verway E J W   +5 more
core   +1 more source

Ruthenium polypyridyl complexes and their modes of interaction with DNA : is there a correlation between these interactions and the antitumor activity of the compounds? [PDF]

, 2008
Various interaction modes between a group of six ruthenium polypyridyl complexes and DNA have been studied using a number of spectroscopic techniques.
Eva Corral, Anna C. G. Hotze, Hans den Dulk, Anna Leczkowska, Alison Rodger, Michael J. Hannon, Jan Reedijk, PJ Loehrer, J Reedijk, J Reedijk, ER Jamieson, J Reedijk, U Bierbach, N Farrell, M Beusichem van, N Farrell, M Coluccia, Y Zou, EI Montero, EI Montero, A Boccarelli, J Kasparkova, J Kasparkova, MJ Clarke, I Kostova, B Armitage, K Davia, B Elias, K Lang, J Reedijk, ACG Hotze, AH Velders, A Habtemariam, RA Vilaplana, RA Vilaplana, G Sava, CG Hartinger, CS Allardyce, V Brabec, E Corral, AEM Boelrijk, PM Vliet van, O Nováková, ME Reichmann, A Kirpal, PA Adcock, YP Keepers, MC Alley, T Mosmann, H Tada, MR Boyd, E Corral, N Grover, SR Smith, A Rodger, SM Kelly, JP Macquet, M Howegrant, V Brabec, G Natile, GI Pascu, HM Chen, K Karidi, H Chao, SR Grguric-Sipka, C Hiort, O Nováková, MJ Hannon, A Rodger, B Nordén, B Nordén, DZM Coggan, I Meistermann, C Uerpmann, MJ Hannon, A Oleksi   +75 more
core   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source