Results 251 to 260 of about 797,307 (281)

Human Lipoprotein Lipase Complementary DNA Sequence

Science, 1987
Lipoprotein lipase is a key enzyme of lipid metabolism that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins. It has been proposed that the enzyme plays a role in the development of obesity and atherosclerosis.
K L, Wion, T G, Kirchgessner, A J, Lusis, M C, Schotz, R M, Lawn   +4 more
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A Drosophila Complementary DNA Resource

Science, 2000
Collections of nonredundant, full-length complementary DNA (cDNA) clones for each of the model organisms and humans will be important resources for studies of gene structure and function. We describe a general strategy for producing such collections and its implementation, which so far has generated a set of cDNAs corresponding to over 40%
G M, Rubin, L, Hong, P, Brokstein, M, Evans-Holm, E, Frise, M, Stapleton, D A, Harvey   +6 more
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“DNA-Dressed NAnopore” for complementary sequence detection

Biosensors and Bioelectronics, 2011
Single molecule electrical sensing with nanopores is a rapidly developing field with potential revolutionary effects on bioanalytics and diagnostics. The recent success of this technology is in the simplicity of its working principle, which exploits the conductance modulations induced by the electrophoretic translocation of molecules through a ...
MUSSI, VALENTINA, P. Fanzio, REPETTO, LUCA, FIRPO, GIUSEPPE, S. Stigliani, G. P. Tonini, VALBUSA, UGO   +6 more
openaire   +2 more sources

Complementary DNA Libraries: An Overview

Molecular Biotechnology, 2003
The generation of complete and full-length cDNA libraries for potential functional assays of specific gene sequences is essential for most molecules in biotechnology and biomedical research. The field of cDNA library generation has changed rapidly in the past 10 yr.
openaire   +3 more sources

Development of a murine hematopoietic progenitor complementary DNA microarray using a subtracted complementary DNA library

Blood, 2002
AbstractWith the goal of creating a resource for in-depth study of myelopoiesis, we have executed a 2-pronged strategy to obtain a complementary DNA (cDNA) clone set enriched in hematopoietic genes. One aspect is a library subtraction to enrich for underrepresented transcripts present at early stages of hematopoiesis.
Xianyong, Ma, Tupur, Husain, Hui, Peng, Sharon, Lin, Olga, Mironenko, Noel, Maun, Steven, Johnson, David, Tuck, Nancy, Berliner, Diane S, Krause, Archibald S, Perkins   +10 more
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Strategies for constructing complementary DNA for cloning

Journal of Theoretical Biology, 1982
Abstract We have examined alternative approaches to existing methods for synthesizing complementary DNA suitable for molecular cloning. One model of construction is presented in which ribonucleotides are added to the 3′ end of complementary DNA prior to synthesis of the second DNA strand.
J, Gaubatz, G V, Paddock
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Gene specific priming of complementary DNA synthesis

Molecular Biology Reports, 1976
DNA, complementary to chicken globin mRNA was synthesized using either Avian Myeloblastosis virus reverse transcriptase, or E. coli DNA polymerase I. Transcriptase cDNA sediments at 9 S on sucrose gradients, and is 620 nucleotides in length, representing a complete copy of globin mRNA template. In contrast, Polymerase I cDNA sediments at 4 S, is 100 to
R J, Crawford, J R, Wells
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Nucleotide Sequence of Human Preproinsulin Complementary DNA

Science, 1980
Recombinant bacterial plasmids that contain DNA complementary to human preproinsulin messenger RNA have been constructed. One clone contains the entire preproinsulin coding region, as well as the 3′ untranslated region of the messenger RNA and eight nucleotides of the 5′ untranslated region.
I, Sures, D V, Goeddel, A, Gray, A, Ullrich   +3 more
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Patents on Random Complementary DNA Fragments?

Science, 1992
The proposal by the National Institutes of Health (NIH) to patent products resulting merely from sequencing the human genome is a mistake: at worst, it is wrong in patent law; at best, it relies on deficiencies in law concerning what is "useful" as a requirement for patents.
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Nonrandom DNA sequencing of exonuclease III-deleted complementary DNA

Analytical Biochemistry, 1985
The nonrandom DNA sequence analysis procedure of Poncz et al. [Proc. Natl. Acad. Sci. USA 79, 4298-4302 (1982)] was extensively modified to permit the determination of complementary DNA (cDNA) sequences containing G-C homopolymer regions. The recombinant cDNA plasmid was cleaved at a unique restriction enzyme site close to the cDNA and treated with ...
openaire   +2 more sources