Development of a method to extract protozoan DNA from black soil
Parasite Epidemiology and Control, 2019 Objectives: Microorganisms in environmental samples are identified by sequential screening, isolation, and culture steps, followed by the verification of physiological characteristics and morphological classification.
Kanako Yamanouchi +3 more
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Protozoan ALKBH8 oxygenases display both DNA repair and tRNA modification activities.
PLoS ONE, 2014 The ALKBH family of Fe(II) and 2-oxoglutarate dependent oxygenases comprises enzymes that display sequence homology to AlkB from E. coli, a DNA repair enzyme that uses an oxidative mechanism to dealkylate methyl and etheno adducts on the nucleobases ...
Daria Zdżalik +10 more
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Albendazole induces oxidative stress and DNA damage in the parasitic protozoan Giardia duodenalis [PDF]
Frontiers in Microbiology, 2015 The control of Giardia duodenalis infections is carried out mainly by drugs, among these albendazole (ABZ) is commonly used. Although the cytotoxic effect of ABZ usually involves binding to β-tubulin, it has been suggested that oxidative stress may also ...
Rodrigo eMartínez-Espinoza +3 more
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Simultaneous Extraction of DNA and RNA from Nuclease-Rich Pathogenic Protozoan Trichomonas vaginalis
BioTechniques, 1996 Chun-Fen Chou, Jung-Hsiang Tai
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Sensing DNA methylation in the protozoan parasite Entamoeba histolytica [PDF]
Molecular Microbiology, 2006 Summary In the protozoan parasite Entamoeba histolytica , 5‐methylcytosine (m5C) was found predominantly in repetitive elements. Its formation is catalysed by Ehmeth, a DNA methyltransferase that belongs to the Dnmt2 subfamily.
Tal, Lavi +5 more
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Sequence specificity of DNA adenine methylase in the protozoan Tetrahymena thermophila [PDF]
Journal of Bacteriology, 1982 The sequence specificity of the Tetrahymena DNA-adenine methylase was determined by nearest-neighbor analyses of in vivo and in vitro methylated DNA. In vivo all four common bases were found to the 5' side of N6-methyladenine, but only thymidine was 3'. Homologous DNA already methylated in vivo and heterologous Micrococcus luteus DNA were methylated in
S, Bromberg, K, Pratt, S, Hattman
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DNA analysis of cattle parasitic protozoan Tritrichomonas foetus after photodynamic therapy [PDF]
Photodiagnosis and Photodynamic Therapy, 2017 Photodynamic therapy (PDT) is a modality of therapy that involves the activation of photosensitive substances and the generation of cytotoxic oxygen species and free radicals to promote the selective destruction of target tissues. This study analyzed the application of PDT to Tritrichomonas foetus, a scourged and etiological agent of bovine ...
Margraf-Ferreira, A. +6 more
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DNA Vaccines against Protozoan Parasites: Advances and Challenges [PDF]
Journal of Biomedicine and Biotechnology, 2007 Over the past 15 years, DNA vaccines have gone from a scientific curiosity to one of the most dynamic research field and may offer new alternatives for the control of parasitic diseases such as leishmaniasis and Chagas disease. We review here some of the advances and challenges for the development of DNA vaccines against these diseases.
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Introduction of plasmid DNA into the trypanosomatid protozoan Crithidia fasciculata. [PDF]
Proceedings of the National Academy of Sciences, 1986 Crithidia fasciculata cells were treated with a plasmid (pDK96) containing pBR322 sequences, a Leishmania tarentolae maxicircle autonomously replicating sequence, and the bacterial gene for aminoglycoside 3' phosphotransferase I inserted between the yeast alcohol dehydrogenase 1 promotor and terminator sequences.
D E, Hughes, L, Simpson
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Homologous Recombination in Protozoan Parasites and Recombinase Inhibitors
Frontiers in Microbiology, 2017 Homologous recombination (HR) is a DNA double-strand break (DSB) repair pathway that utilizes a homologous template to fully repair the damaged DNA. HR is critical to maintain genome stability and to ensure genetic diversity during meiosis. A specialized
Andrew A. Kelso +7 more
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