Introduction of plasmid DNA into the trypanosomatid protozoan Crithidia fasciculata. [PDF]
Crithidia fasciculata cells were treated with a plasmid (pDK96) containing pBR322 sequences, a Leishmania tarentolae maxicircle autonomously replicating sequence, and the bacterial gene for aminoglycoside 3' phosphotransferase I inserted between the yeast alcohol dehydrogenase 1 promotor and terminator sequences.
D E, Hughes, L, Simpson
openaire +2 more sources
Homologous Recombination in Protozoan Parasites and Recombinase Inhibitors
Homologous recombination (HR) is a DNA double-strand break (DSB) repair pathway that utilizes a homologous template to fully repair the damaged DNA. HR is critical to maintain genome stability and to ensure genetic diversity during meiosis. A specialized
Andrew A. Kelso +7 more
doaj +1 more source
Molecular and phylogenetic characterization of honey bee viruses, Nosema microsporidia, protozoan parasites, and parasitic mites in China [PDF]
China has the largest number of managed honey bee colonies, which produce the highest quantity of honey and royal jelly in the world; however, the presence of honey bee pathogens and parasites has never been rigorously identified in Chinese apiaries.
Yang, Bu +3 more
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Application of next generation sequencing for detection of protozoan pathogens in shellfish
Food and waterborne protozoan pathogens can cause serious disease in people. Three common species Cryptosporidium parvum, Giardia enterica and Toxoplasma gondii can contaminate diverse shellfish species, including commercial oysters.
Catherine DeMone +8 more
doaj +1 more source
An unusually compact ribosomal DNA repeat in the protozoanGiardia lamblia [PDF]
The ribosomal RNA (rRNA) genes of the protozoan parasite Giardia lamblia have been analyzed with respect to size, composition and copy number. They are found to be remarkable in several respects. First, the rRNAs themselves are the smallest yet reported for any eukaryotic organism.
J C, Boothroyd +3 more
openaire +2 more sources
Detection of Babesia bigemina infection: use of a DNA probe - a review
The development of a repetitive DNA probe for Babesia bigemina was reviewed. The original plasmid (p(Bbi)16) contained an insert of B. bigemina DNA of approximately 6.3 kb.
Gerald M. Buening, Julio V. Figueroa
doaj +1 more source
Molecular Identification and Genotyping of Theileria Orientalis Type 3 (Buffeli) Isolated from Cattle Using Nested-PCR Assay in Guilan Province, Iran [PDF]
Protozoan parasites of the genus Theileria are tick-borne parasites that have been found in many species of mammals. More than a dozen species of Theileria have been found in cattle, water buffalo, sheep, and goats.
Gh Habibi +4 more
doaj +1 more source
Detection of protozoan hosts for Legionella pneumophila in engineered water systems by using a biofilm batch test [PDF]
Legionella pneumophila proliferates in aquatic habitats within free-living protozoa, 17 species of which have been identified as hosts by using in vitro experiments. The present study aimed at identifying protozoan hosts for L.
Valster, R.M. +2 more
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Entamoeba histolytica, the protozoan responsible for human amoebiasis, exhibits a great genome plasticity that is probably related to homologous recombination events.
del Socorro Charcas-Lopez Ma. +5 more
doaj +1 more source
Cloning and expression of codon-optimized recombinant darbepoetin alfa in Leishmania tarentolae T7-TR [PDF]
Darbepoetin alfa is an engineered and hyperglycosylated analog of recombinant human erythropoietin (EPO) which is used as a drug in treating anemia in patients with chronic kidney failure and cancer.
Fard-Esfahani, P. +8 more
core +1 more source

