Results 201 to 210 of about 77,140 (213)
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Molecular and Biochemical Parasitology, 1990
Cysteine protease gene fragments from three protozoan parasites Trypanosoma cruzi, Trypanosoma brucei, and Entamoeba histolytica were amplified by the polymerase chain reaction (PCR) from genomic DNA using degenerate oligonucleotide primers. The primers used for the amplification were designed based upon amino acid sequences flanking the active site ...
A E, Eakin +4 more
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Cysteine protease gene fragments from three protozoan parasites Trypanosoma cruzi, Trypanosoma brucei, and Entamoeba histolytica were amplified by the polymerase chain reaction (PCR) from genomic DNA using degenerate oligonucleotide primers. The primers used for the amplification were designed based upon amino acid sequences flanking the active site ...
A E, Eakin +4 more
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Regulation of DNA replication proteins in parasitic protozoans: possible role of CDK-like kinases
Current Genetics, 2016Regulatory roles of CDKs in fundamental processes including cell cycle progression and transcription are well conserved in metazoans. This family of proteins has undergone significant evolutionary divergence and specialization. Several CDK-like kinases have been identified and characterized in parasitic protozoans.
Abhijit S, Deshmukh +2 more
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Molecular Pharmaceutics, 2020
DNA vaccinations are promising strategies for treating diseases that require cellular immunity (i.e., cancer and protozoan infection). Here, we report on the use of a liposomal nanocarrier (lipid nanoparticles (LNPs)) composed of an SS-cleavable and pH-activated lipidlike material (ssPalm) as an in vivo DNA vaccine.
Mio Maeta +13 more
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DNA vaccinations are promising strategies for treating diseases that require cellular immunity (i.e., cancer and protozoan infection). Here, we report on the use of a liposomal nanocarrier (lipid nanoparticles (LNPs)) composed of an SS-cleavable and pH-activated lipidlike material (ssPalm) as an in vivo DNA vaccine.
Mio Maeta +13 more
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DNA methylation in vegetative and conjugating cells of a protozoan ciliate: Blepharisma japonicum
Developmental Genetics, 1986AbstractWe report here the presence of N6‐methyladenine (MeAde) in the macronuclear DNA (maDNA) of Blepharisma japonicum vegetative cells. We have further investigated the relationship between DNA methylation and cell union in cells activated for conjugation. Such activation was induced by treating cells of mating type I with complementary gamone 2. We
SALVINI, Mariangela +3 more
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Journal of Experimental Zoology, 1987
AbstractSome species of ciliated protozoans vary their body volume when fed on preys of different sizes. This peculiarity has offered us the opportunity to examine the relationship between changes in cell volume, amount of macronuclear DNA (maDNA), and cell division rates in subclones derived from a single clone of the ciliate Blepharisma japonicum ...
SANTANGELO, GIOVANNI, Barone E.
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AbstractSome species of ciliated protozoans vary their body volume when fed on preys of different sizes. This peculiarity has offered us the opportunity to examine the relationship between changes in cell volume, amount of macronuclear DNA (maDNA), and cell division rates in subclones derived from a single clone of the ciliate Blepharisma japonicum ...
SANTANGELO, GIOVANNI, Barone E.
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Fast and reliable extraction of protozoan parasite DNA from fecal specimens.
Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology, 1999Polymerase chain reaction (PCR) detection of intestinal protozoa in fecal specimens is hampered by poor recovery of DNA and by the presence of PCR inhibitors. In this study we describe a novel method for DNA extraction from such specimens containing spores and oocysts of Enterocytozoon bieneusi and Cryptosporidium parvum, respectively.Extraction was ...
A J, da Silva +5 more
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Trends in Parasitology, 2004
DNA microarray analysis has been successfully applied to most of the protozoan parasites that cause human disease, but has not made equal progress in all cases. The results for kinetoplastid parasites (Leishmania and Trypanosoma) are primarily at the stage of validation and new gene discovery.
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DNA microarray analysis has been successfully applied to most of the protozoan parasites that cause human disease, but has not made equal progress in all cases. The results for kinetoplastid parasites (Leishmania and Trypanosoma) are primarily at the stage of validation and new gene discovery.
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DNA-dependent RNA Polymerase from a Protozoan, Tetrahymena pyriformis
The Journal of Biochemistry, 1975Toru HIGASHINAKAGAWA +2 more
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Journal of biochemistry, 1975
Three peaks of DNA-dependent RNA polymerase (EC 2.7.7.6) activity were resolved when the enzyme was prepared from the isolated macronuclei of Tetrahymena pyriformis GL(amicronucleate strain) and chromatographed on DEAE-Sephadex A25. They were eluted at around 0.05, 0.15, and 0.2 M of ammonium sulfate, and termed TIa, TIb, and TII, respectively.
T, Higashinakagawa, F, Tashiro, T, Mita
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Three peaks of DNA-dependent RNA polymerase (EC 2.7.7.6) activity were resolved when the enzyme was prepared from the isolated macronuclei of Tetrahymena pyriformis GL(amicronucleate strain) and chromatographed on DEAE-Sephadex A25. They were eluted at around 0.05, 0.15, and 0.2 M of ammonium sulfate, and termed TIa, TIb, and TII, respectively.
T, Higashinakagawa, F, Tashiro, T, Mita
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Fast and reliable extraction of protozoan parasite DNA from fecal specimens2
Molecular Diagnosis, 1999A DASILVA +5 more
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