Results 191 to 200 of about 76,669 (236)
Molecular detection of <i>Toxoplasma gondii</i> in <i>Cerdocyon thous</i> at southern Brazil. [PDF]
Braz J Vet MedLignon JS +10 more
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DNA topoisomerases in the unicellular protozoan parasites: Unwinding the mystery
Biochemical Pharmacology, 2022DNA topoisomerases are a group of enzymes present ubiquitously in all organisms from unicellular protozoan parasites to humans. These enzymes control the topological problems caused by DNA double helix in the cell during nucleic acid metabolism.
Swati, Lamba, Amit, Roy
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DNA Polymerases in Parasitic Protozoans Differ from Host Enzymes
Science, 1980Analysis of extracts of the bloodstream forms of Trypanosoma brucei showed that both DNA polymerase-α and DNA polymerase-β activities were present. The detection of DNA polymerase-β in T. brucei demonstrates the presence of this enzyme in unicellular organisms.
L M, Chang +3 more
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Drug Discovery Today, 2023
DNA topoisomerases are a group of enzymes omnipresent in all organisms. They maintain the DNA topology during replication, repair, recombination, and transcription. However, the structure of topoisomerase in protozoan parasites differs significantly from that of human topoisomerases; thus, this enzyme acts as a crucial target in drug development ...
Swati, Lamba, Amit, Roy
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DNA topoisomerases are a group of enzymes omnipresent in all organisms. They maintain the DNA topology during replication, repair, recombination, and transcription. However, the structure of topoisomerase in protozoan parasites differs significantly from that of human topoisomerases; thus, this enzyme acts as a crucial target in drug development ...
Swati, Lamba, Amit, Roy
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Dna-Protein Interactions at Telomeres in Ciliated Protozoans
Advances in Molecular and Cell Biology, 1988Publisher Summary This chapter discusses the DNA–protein complexes found at telomeres in the macronuclei of ciliated protozoans, with special attention given to studies in Oxytricha (a hypotrichous ciliate) and Tetrahymena (a holotrichous ciliate). The structure of telomeres has been largely elucidated by studies on macronuclear DNA from ciliated
D GOTTSCHLING, V ZAKIAN
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Stable DNA transfection of the primitive protozoan pathogen Giardia lamblia
Molecular and Biochemical Parasitology, 1998We have developed a stable DNA transfection vector pRANneo for genetic manipulation of the primitive protozoan Giardia lamblia. pRANneo was constructed by replacing the protein coding region of a Giardia ran gene with a bacterial neomycin phosphotransferase gene (neo). This plasmid was electroporated into G. lamblia, and the transfectants were selected
C H, Sun, C F, Chou, J H, Tai
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DNA microarray-based detection and identification of waterborne protozoan pathogens
Journal of Microbiological Methods, 2010A DNA microarray with 21 oligonucleotide probes was developed to detect most of the common waterborne protozoan pathogens. The DNA microarray accurately identified 3 test protozoa strains based on the small subunit (SSU) rRNA gene sequence. The detection limit was approximately 1x10(3) target genes, or 50 Cryptosporidium parvum oocysts, per assay. As a
Dae-Young, Lee +2 more
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Stimulation of B lymphocytes, macrophages, and dendritic cells by protozoan DNA
Microbes and Infection, 2002This review summarizes recent work from our laboratory demonstrating the activation of B lymphocytes, macrophages and dendritic cells by DNA from three different protozoan parasites of cattle and humans that is qualitatively similar to the now well-described effects of CpG-containing bacterial DNA. This novel mechanism of protozoan parasite recognition
Wendy C, Brown, Ricardo S, Corral
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Use of Hybridoma Antibodies and Recombinant DNA Technology in Protozoan Vaccine Development
Avian Diseases, 1986The use of hybridoma antibodies developed against the sporozoite stage of avian coccidia, coupled with genetic-engineering techniques, has made it possible to begin bird-immunization studies utilizing an Escherichia coli-elicited coccidial protein. The coccidia are currently controlled in the poultry industry by use of anticoccidial compounds, but it ...
H D, Danforth, P C, Augustine
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Talanta, 1997
An electrochemical hybridization biosensor was developed for the detection of short DNA fragments specific to the deadly waterborne pathogen Cryptosporidium. The sensor relies on the immobilization of a 38-mer oligonucleotide unique to the Cryptosporidium DNA onto the carbon-paste transducer, and employs a highly sensitive chronopotentiometric ...
J, Wang +4 more
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An electrochemical hybridization biosensor was developed for the detection of short DNA fragments specific to the deadly waterborne pathogen Cryptosporidium. The sensor relies on the immobilization of a 38-mer oligonucleotide unique to the Cryptosporidium DNA onto the carbon-paste transducer, and employs a highly sensitive chronopotentiometric ...
J, Wang +4 more
openaire +2 more sources