Results 201 to 210 of about 47,206 (236)
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Use of Hybridoma Antibodies and Recombinant DNA Technology in Protozoan Vaccine Development
Avian Diseases, 1986The use of hybridoma antibodies developed against the sporozoite stage of avian coccidia, coupled with genetic-engineering techniques, has made it possible to begin bird-immunization studies utilizing an Escherichia coli-elicited coccidial protein. The coccidia are currently controlled in the poultry industry by use of anticoccidial compounds, but it ...
H D, Danforth, P C, Augustine
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Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1988
1. A method for isolating DNA from Pneumocystis carinii is described. 2. The DNA content per nucleus is 0.22-0.34 pg. 3. This finding is consistent with other parasitic protozoa DNA content per nuclei.
M S, Gradus, M, Gilmore, M, Lerner
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1. A method for isolating DNA from Pneumocystis carinii is described. 2. The DNA content per nucleus is 0.22-0.34 pg. 3. This finding is consistent with other parasitic protozoa DNA content per nuclei.
M S, Gradus, M, Gilmore, M, Lerner
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Talanta, 1997
An electrochemical hybridization biosensor was developed for the detection of short DNA fragments specific to the deadly waterborne pathogen Cryptosporidium. The sensor relies on the immobilization of a 38-mer oligonucleotide unique to the Cryptosporidium DNA onto the carbon-paste transducer, and employs a highly sensitive chronopotentiometric ...
J, Wang +4 more
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An electrochemical hybridization biosensor was developed for the detection of short DNA fragments specific to the deadly waterborne pathogen Cryptosporidium. The sensor relies on the immobilization of a 38-mer oligonucleotide unique to the Cryptosporidium DNA onto the carbon-paste transducer, and employs a highly sensitive chronopotentiometric ...
J, Wang +4 more
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DNA methylation in vegetative and conjugating cells of a protozoan ciliate: Blepharisma japonicum
Developmental Genetics, 1986AbstractWe report here the presence of N6‐methyladenine (MeAde) in the macronuclear DNA (maDNA) of Blepharisma japonicum vegetative cells. We have further investigated the relationship between DNA methylation and cell union in cells activated for conjugation. Such activation was induced by treating cells of mating type I with complementary gamone 2. We
SALVINI, Mariangela +3 more
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Molecular and Biochemical Parasitology, 1990
Cysteine protease gene fragments from three protozoan parasites Trypanosoma cruzi, Trypanosoma brucei, and Entamoeba histolytica were amplified by the polymerase chain reaction (PCR) from genomic DNA using degenerate oligonucleotide primers. The primers used for the amplification were designed based upon amino acid sequences flanking the active site ...
A E, Eakin +4 more
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Cysteine protease gene fragments from three protozoan parasites Trypanosoma cruzi, Trypanosoma brucei, and Entamoeba histolytica were amplified by the polymerase chain reaction (PCR) from genomic DNA using degenerate oligonucleotide primers. The primers used for the amplification were designed based upon amino acid sequences flanking the active site ...
A E, Eakin +4 more
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Gene, 1996
A composite 1876-bp DNA encoding a putative protein disulfide isomerase (PDI) has been constructed from clones isolated from Cryptosporidium parvum (C. parvum) genomic and cDNA libraries and the nucleotide sequence determined. As predicted from the open reading frame (ORF), the protein product has a predicted molecular size of 54 kDa and a high degree ...
D S, Blunt +3 more
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A composite 1876-bp DNA encoding a putative protein disulfide isomerase (PDI) has been constructed from clones isolated from Cryptosporidium parvum (C. parvum) genomic and cDNA libraries and the nucleotide sequence determined. As predicted from the open reading frame (ORF), the protein product has a predicted molecular size of 54 kDa and a high degree ...
D S, Blunt +3 more
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Regulation of DNA replication proteins in parasitic protozoans: possible role of CDK-like kinases
Current Genetics, 2016Regulatory roles of CDKs in fundamental processes including cell cycle progression and transcription are well conserved in metazoans. This family of proteins has undergone significant evolutionary divergence and specialization. Several CDK-like kinases have been identified and characterized in parasitic protozoans.
Abhijit S, Deshmukh +2 more
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Fast and reliable extraction of protozoan parasite DNA from fecal specimens.
Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology, 1999Polymerase chain reaction (PCR) detection of intestinal protozoa in fecal specimens is hampered by poor recovery of DNA and by the presence of PCR inhibitors. In this study we describe a novel method for DNA extraction from such specimens containing spores and oocysts of Enterocytozoon bieneusi and Cryptosporidium parvum, respectively.Extraction was ...
A J, da Silva +5 more
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Trends in Parasitology, 2004
DNA microarray analysis has been successfully applied to most of the protozoan parasites that cause human disease, but has not made equal progress in all cases. The results for kinetoplastid parasites (Leishmania and Trypanosoma) are primarily at the stage of validation and new gene discovery.
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DNA microarray analysis has been successfully applied to most of the protozoan parasites that cause human disease, but has not made equal progress in all cases. The results for kinetoplastid parasites (Leishmania and Trypanosoma) are primarily at the stage of validation and new gene discovery.
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1986
Xenosome is the term we have adopted to describe a group of infectious intracellular bacterial endosymbionts found in several strains of the marine ciliate Parauronema acutum. Whereas symbionts of each strain are capable of infecting homologous and heterologous protozoan stocks, xenosomes from only certain strains are capable of killing other marine ...
A. T. Soldo, S. A. Brickson, A. Freytag
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Xenosome is the term we have adopted to describe a group of infectious intracellular bacterial endosymbionts found in several strains of the marine ciliate Parauronema acutum. Whereas symbionts of each strain are capable of infecting homologous and heterologous protozoan stocks, xenosomes from only certain strains are capable of killing other marine ...
A. T. Soldo, S. A. Brickson, A. Freytag
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