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Optimization and production of a GLP-grade recombinant Sm29 vaccine against schistosomiasis: From expression to preclinical assessment. [PDF]

PLoS Negl Trop Dis
Andrade JM, Mambelli F, Gomes DS, Souza MFS, Fahel JS, Sanches RCO, Marinho FV, Salazar N, Gomes IP, Martins VT, Carvalho EM, de Magalhaes MTQ, Teixeira SR, Oliveira SC.   +13 more
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Scarless DNA Recombineering

Cold Spring Harbor Protocols, 2023
The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi, Roberto Balbontín, Lionello Bossi   +2 more
openaire   +2 more sources

Recombination in yeast and the recombinant DNA technology

Genome, 1989
The development of methods to isolate eukaryotic genes, alter these genes in vitro and reintroduce them into the cell has had a major impact on the study of recombination in the yeast Saccharomyces cerevisiae. In this paper we discuss how recombinant DNA techniques have been employed in the study of recombination in yeast and the results that have ...
T D, Petes, P, Detloff, S, Jinks-Robertson, S R, Judd, M, Kupiec, D, Nag, A, Stapleton, L S, Symington, A, Vincent, M, White   +9 more
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DNA Recombineering Applications

Cold Spring Harbor Protocols, 2023
The ability to manipulate the bacterial genome is an obligatory premise for the study of gene function and regulation in bacterial cells. The λ red recombineering technique allows modification of chromosomal sequences with base-pair precision without the need of intermediate molecular cloning steps.
Nara Figueroa-Bossi, Roberto Balbontín, Lionello Bossi   +2 more
openaire   +2 more sources

Introduction to recombinant DNA

Journal of Inherited Metabolic Disease, 1986
AbstractThis paper describes the current state of knowledge of methods for analysing gene structure and localization. Illustrations are given of the preparation of complementary DNA libraries and their screening by positive‐negative selection, the use of synthetic oligodeoxynucleotides and the use of antibodies. Analysis of the EGF precursor is used as
openaire   +2 more sources