Study of the 16S–23S ribosomal DNA internal spacer of Coxiella burnetii
European Journal of Epidemiology, 1997The complete 16S-23S ribosomal DNA (rDNA) internal transcribed spacer (ITS) of 22 isolates of the obligate intracellular bacterium Coxiella burnetii, the agent of Q fever, were amplified by the polymerase chain reaction (PCR) and sequenced using an automated laser fluorescent DNA sequencer.
A, Stein +3 more
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Authentication of Medicinal Dendrobium Species by the Internal Transcribed Spacer of Ribosomal DNA
Planta Medica, 2001Herba Dendrobii (Shihu) is a commonly used Chinese medicine derived from the stem of several orchid species belonging to the genus Dendrobium. It is rather expensive and adulteration is frequent. Proper authentication of the medicinal species is necessary to protect consumers and support conservation measures.
D T, Lau, P C, Shaw, J, Wang, P P, But
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Structure of the central spacer region of extrachromosomal ribosomal DNA in Physarum polycephalum
Journal of Molecular Biology, 1982Abstract We have analyzed the sequence organization of the central spacer region of the extrachromosomal ribosomal DNA from two strains of the acellular slime mold Physarum polycephalum . It had been inferred previously from electron microscopy that this region, which comprises about one third of the 60 kb ‡ palindromic rDNA, contains a complex ...
P J, Ferris, V M, Vogt
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Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis
Cell, 1979A detailed restriction map was constructed for a cloned Xenopus laevis rDNA fragment containing the nontranscribed spacer (NTS) and external transcribed spacer (ETS) together with a portion of both the 18S and 28S rRNA genes. The NTS was found to contain at least three distinct repetitious areas.
Boseley, Paul +4 more
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The Cucurbita maxima ribosomal DNA intergenic spacer has a complex structure
Gene, 1989The nucleotide (nt) sequence of the 5508-nt intergenic spacer (IGS), between the 25S- and the 18S-coding regions of Cucurbita maxima rDNA, was determined. The fragment sequenced is 6142 nt long and includes 472 nt of 25S- and 162 nt of 18S-coding regions.
R J, Kelly, A, Siegel
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Genotyping of Trichophyton rubrum by analysis of ribosomal-DNA intergenic spacer regions
Mycopathologia, 2007An attempt was made to explore the genotyping of Trichophyton rubrum (T. rubrum) and the relationship between genotype and geographical origin using ribosomal restriction endonuclease polymorphic analysis. The total DNA was extracted by cetyltrimethyl ammonium bromide (CTAB). The probe was amplified from part of the 18S, ITSI, 5.8S, and ITSII region of
Guoling, Yang +6 more
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X and Y chromosomal ribosomal DNA of drosophila: comparison of spacers and insertions
Cell, 1978Abstract In Drosophila melanogaster, the genes coding for 18S and 28S ribosomal RNA (rDNA) are clustered at one locus each on the X and the Y chromosomes. We have compared the structure of rDNA at the two loci. The 18S and 28S rRNAs coded by the X and Y chromosomes are very similar and probably identical (Maden and Tartof, 1974). In D.
P K, Wellauer, I B, Dawid, K D, Tartof
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Sequence Variation in the Ribosomal DNA Internal Transcribed Spacer of Tridacna crocea
Marine Biotechnology, 2000DNA-based genetic markers are needed to augment existing allozyme markers in the assessment of genetic diversity of wild giant clam populations. The dearth of polymorphic mitochondrial DNA regions amplified from known universal polymerase chain reaction (PCR) primers has led us to search other regions of the genome for viable sources of DNA ...
E T, Yu, M A, Juinio-Meñez, V D, Monje
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Fourteen internal transcribed spacers in the circular ribosomal DNA of Euglena gracilis
Journal of Molecular Biology, 1990Cytoplasmic ribosomes from Euglena gracilis contain 16 rRNA components. These include the typical 5 S, 5.8 S and 19 S rRNAs that are found in other eukaryotes as well as 13 discrete small RNAs that interact to form the equivalent of eukaryotic 25-28 S rRNA (accompanying paper).
M N, Schnare, J R, Cook, M W, Gray
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Length variation in the internal transcribed spacer of ribosomal DNA in chanterelles
Mycological Research, 1994DNA was extracted from 31 basidiomes of Cantharellus, Craterellus , and other basidiomycetes considered to have affinities to the Cantharellaceae. The internal transcribed spacer (ITS) between the 18S and the 28S genes of the nuclear ribosomal DNA was amplified using the polymerase chain reaction.
Toby Feibelman +2 more
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