Results 51 to 60 of about 35,489 (287)

Oligonucleotide separation techniques for purification and analysis: What can we learn for today's tasks?

ELECTROPHORESIS, Volume 43, Issue 23-24, Page 2402-2427, December 2022., 2022
Abstract Nucleic acids are the blueprint of life. They are not only the construction plan of the single cell or higher associations of them, but also necessary for function, communication and regulation. Due to the pandemic, the attention shifted in particular to their therapeutic potential as a vaccine. As pharmaceutical oligonucleotides are unique in
Robert Minkner, Jirayu Boonyakida, Enoch Y. Park, Hermann Wätzig   +3 more
wiley   +1 more source

Kinetic Proofreading and the Limits of Thermodynamic Uncertainty [PDF]

Phys. Rev. E 101, 022415 (2020), 2019
To mitigate errors induced by the cell's heterogeneous noisy environment, its main information channels and production networks utilize the kinetic proofreading (KPR) mechanism. Here, we examine two extensively-studied KPR circuits, DNA replication by the T7 DNA polymerase and translation by the E. coli ribosome. Using experimental data, we analyze the
arxiv   +1 more source

Species delimitation in the African Armillaria complex by analysis of the ribosomal DNA spacers.

The Journal of General and Applied Microbiology, 1997
Thirteen Armillaria isolates, collected from various geographical areas in tropical Africa and previously characterized by cultural morphology, pairing tests and isozyme analysis, were evaluated using the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).
Mohammed Chillali, Agustian, Hakima Idder-Ighili, Jean-Jacques Guillauminz, Jean-Jacques Guillauminz, Bernard Botton, Caroline Mohammed   +6 more
openaire   +6 more sources

Response to neoadjuvant chemotherapy in early breast cancers is associated with epithelial–mesenchymal transition and tumor‐infiltrating lymphocytes

Molecular Oncology, EarlyView.
Epithelial–mesenchymal transition (EMT) and tumor‐infiltrating lymphocytes (TILs) are associated with early breast cancer response to neoadjuvant chemotherapy (NAC). This study evaluated EMT and TIL shifts, with immunofluorescence and RNA sequencing, at diagnosis and in residual tumors as potential biomarkers associated with treatment response.
Françoise Derouane, Jérôme Ambroise, Cédric van Marcke, Mieke Van Bockstal, Martine Berlière, Christine Galant, Hélène Dano, Médina Lougué, Elena Benidovskaya, Guy Jerusalem, Vincent Bours, Claire Josse, Jérôme Thiry, Aurélie Daumerie, Caroline Bouzin, Cyril Corbet, François P. Duhoux   +16 more
wiley   +1 more source

Coarse-Grained model of the demixing of DNA and non-binding globular macromolecules [PDF]

Journal of Physical Chemistry B 121 (2017) 6351-6358, 2017
The volume occupied by the unconstrained genomic DNA of prokaryotes in saline solutions is thousand times larger than the cell. Moreover, it is not separated from the rest of the cell by a membrane. Nevertheless, it occupies only a small fraction of the cell called the nucleoid.
arxiv   +1 more source

Fixation Times of Retroposons in the Ribosomal DNA Spacer of Human and Other Primates

Genomics, 1993
We have investigated the presence/absence of two types of retroposed sequences found in human ribosomal DNA in equivalent positions in chimpanzee, gorilla, orangutan, gibbon, and rhesus monkey rDNA. These sequences are one pseudogene derived from the single-copy cdc27hs gene and seven complete Alu elements.
James E. Sylvester, Philip Hieter, Iris L. Gonzalez, Stuart Tugendreich   +3 more
openaire   +3 more sources

A companion to the preclinical common data elements for genomics, transcriptomics, and epigenomics data in rodent epilepsy models. A report of the TASK3‐WG4 omics working group of the ILAE/AES joint translational TASK force

Epilepsia Open, EarlyView., 2022
Abstract The International League Against Epilepsy/American Epilepsy Society (ILAE/AES) Joint Translational Task Force established the TASK3 working groups to create common data elements (CDEs) for various preclinical epilepsy research disciplines. The aim of the CDEs is to improve the standardization of experimental designs across a range of epilepsy ...
Erwin A. van Vliet, Michael S. Hildebrand, James D. Mills, Gary P. Brennan, Tore Eid, Susan A. Masino, Vicky Whittemore, Laura Bindila, Kevin K. Wang, Manisha Patel, Piero Perucca, Christopher A. Reid   +11 more
wiley   +1 more source

Genetic diversity of ‘Candidatus Phytoplasma mali’ strains in Poland

Phytopathologia Mediterranea, 2015
During 2010-2013, samples from 267 apple trees growing in six regions of Poland were tested for phytoplasma presence. ‘Candidatus Phytoplasma mali’ was detected in 17 samples from phloem tissue of apple shoots showing typical apple proliferation symptoms.
Mirosława CIEŚLIŃSKA, Ewa HENNIG, Dorota KRUCZYŃSKA, Assunta BERTACCINI   +3 more
doaj   +1 more source

Variability of Human rDNA

Cells, 2021
In human cells, ribosomal DNA (rDNA) is arranged in ten clusters of multiple tandem repeats. Each repeat is usually described as consisting of two parts: the 13 kb long ribosomal part, containing three genes coding for 18S, 5.8S and 28S RNAs of the ...
Evgeny Smirnov, Nikola Chmúrčiaková, František Liška, Pavla Bažantová, Dušan Cmarko   +4 more
doaj   +1 more source

Consolidate Overview of Ribonucleic Acid Molecular Dynamics: From Molecular Movements to Material Innovations

Advanced Engineering Materials, EarlyView.
Molecular dynamics simulations are advancing the study of ribonucleic acid (RNA) and RNA‐conjugated molecules. These developments include improvements in force fields, long‐timescale dynamics, and coarse‐grained models, addressing limitations and refining methods.
Kanchan Yadav, Iksoo Jang, Jong Bum Lee
wiley   +1 more source