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Application of Magnetic Bead Extraction Technology in Detection of Vibrio parahaemolyticus(磁珠提取技术在副溶血弧菌检测中的应用) [PDF]
Shipin kexue jishu xuebao, 2021 Vibrio parahaemolyticus is a common food borne pathogen. In order to solve the problems of complex steps and long detection period of Vibrio parahaemolyticus, a magnetic bead extraction and PCR combined technique was developed for rapid detection of ...
XIAO Yushi(肖雨诗) +6 more
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Bio-molecules Damage Protective Effect and Free Radical Scavenging Capacity of Coriandrum sativum L.(芫荽对生物大分子氧化损伤的保护作用及自由基清除能力) [PDF]
Shipin kexue jishu xuebao, 2017 In order to study bio-molecules damage protective effect and free radical scavenging capacity of Coriandrum sativum L. (coriander), Folin-phenol assay was used for estimating total polyphenol of coriander.
HAN Yingyi(韩潆仪) +4 more
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Identification of Shark Fin Using 12S rRNA Gene Marker(利用12S rRNA基因标记鉴定鱼翅真伪) [PDF]
Shipin kexue jishu xuebao, 2017 To detect authenticity and species of 23 suspected shark fin samples. DNAs from either shark fin or shark fin derived soup were extracted, and then 12S rRNA gene of mitochondrial DNA that was special for animal species identification was amplified.
HUANG Yalin(黄娅琳)
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Zhongguo shiyan zhenduanxue, 2011 目的对阴道加德纳菌基因组DNA几种不同的提取方法进行比较,以利于细菌性阴道病的研究。方法采用几种不同方法提取阴道加德纳菌基因组DNA,对不同方法提取的细菌基因组DNA进行定量检测,以评价不同方法的提取效率。结果加德纳菌基因组DNA提取以改良法提取率较高,酚-氯仿抽提法、醋酸钾法次之,碱裂解煮沸法和溶菌酶煮沸法提取效率较差,加热煮沸法提取效率低。结论改良法提取GV基因组DNA效率较好,适用于临床应用。
姚虹, 吕治, 苏建荣
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Zhongguo shiyan zhenduanxue, 2006 目的为寻找一种高效、简便、实用的石蜡组织中DNA提取方法,本研究比较了三种不同的DNA提取方法对DNA质量的影响。方法选取淋巴相关组织的蜡块21例。三种DNA提取方法分别是简单消化煮沸法,酚/氯仿提纯法和试剂盒法。通过提取DNA的浓度和纯度、PCR扩增保守序列β-globin等方法,比较三种DNA提取方法的优劣。结果试剂盒法提取样品OD260/OD280平均比值位于1.61.8之间;提纯法位于1.31.5之间;简单消化煮沸法位于1.21.3之间。三种方法中,试剂盒法提取的DNA最为稳定 ...
齐宗利 +4 more
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Zhejiang Daxue xuebao. Lixue ban, 2013 为了建立一种检测水中铜绿微囊藻浓度的定量PCR方法，分别用SDS裂解法和DNeasy Blood & Tissue Kit试剂盒提取标准品的DNA并建立标准曲线.结果表明采用DNeasy Blood & Tissue Kit试剂盒提取的标准品DNA具有很好的重复性，经定量PCR扩增得到的标准曲线R2为0.968，可以用来检测样品中铜绿微囊藻的浓度，为建立一套长期有效的测定水中铜绿微囊藻浓度的监测体系提供了技术参考.
ZHUJin-yu(朱金余) +3 more
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Zhongguo shiyan zhenduanxue, 2018 目的探讨在高海拔地区应用血液基因组DNA提取试剂盒的最佳条件。方法应用RelaxGene Blood DNA System血液基因组DNA提取系统,对109份血样(每份血样分为2组,实验组和对照组)提取DNA,实验组采用优化条件提取DNA、对照组采用试剂盒说明书方法提取DNA。结果实验组109份样品中有13份DNA浓度在20-50ng/μL之间,35份在51-80ng/μL之间,61份样品>80ng/μL;DNA浓度比例高于对照组 ...
范东艳, 杜宝中, 王苹, 全雄杰
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A rapid soil DNA extraction method applied to field detection(一种适用于现场检测的土壤DNA快速提取方法(英文))
浙江大学学报. 农业与生命科学版Soil DNA extraction, such as microbial community analysis and gene drift detection, is an important basis for multiple analyses in different fields. Nevertheless, the soil DNA extraction methods for field detection are still lacking.
YAN Jingying(颜晶莹) +3 more
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Zhongguo shiyan zhenduanxue, 2006 目的比较三种快速、简便、适用、低成本的从全血中提取基因组DNA的方法。方法选用快速氯仿-异戊醇法、简化盐析法、改良碘化钾法直接从全血中提取基因组DNA。结果改良碘化钾法提取的DNA纯度及含量最高,快速氯仿-异戊醇法次之,简化盐析法则相对较低。结论改良碘化钾法为较好的提取基因组DNA的方法。
涂向东, 江清华, 兰风华
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Zhongguo shiyan zhenduanxue, 2009 目的通过比较两种从石蜡包埋组织中提取基因组DNA的方法,确定一种高效、经济而简便的方法指导实际工作。方法按照两种方法（脱蜡法与非脱蜡法）提取DNA,比较所提DNA的质量,PCR扩增β-Globin基因作为基因分析的对照扩增,并对其中非脱蜡法进行改良。结果两种方法所提取的DNA均能达到相关分子学实验的要求,PCR扩增带清晰,结果较为相似。结论非脱蜡法也能提取出适用于PCR技术要求的DNA,方法简便、经济。
王卡娜 +4 more
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