Results 31 to 40 of about 4,740 (167)
野生动物学报, 2010 褐马鸡是中国特有的国家Ⅰ级保护濒危鸟类,为了解决野生褐马鸡分子生物学研究中存在的取样局限性,采用非伤害性取样和非损伤性取样,对褐马鸡微量血液、皮、脚垫、指甲、羽毛、陈旧标本的皮以及粪便的DNA提取进行了初步研究。结果表明,从褐马鸡微量血液（10μL）中可以提取到浓度和纯度高的基因组DNA（41.85μg）;从褐马鸡的皮、脚垫、指甲、羽毛中可以提取出基因组DNA并扩增出目标片断;陈旧标本的皮能够提取到基因组DNA。该研究将拓宽褐马鸡分子生物学研究的采样范围,并可以提高野外非损伤性取样在保护遗传学中的应用。
武玉珍, 王孟本, 张峰
doaj
TNF induced RIP3-dependent NADH depletion [PDF]
, 2015 肿瘤坏死因子（tumornecrosisfactor，TNF）能诱导L929细胞产生相互作用蛋白3（receptor-interactingprotein3，RIP3）依赖的程序性细胞坏死，并且在该过程中，胞内的线粒体产生的活性氧（reactiveoxygenspecies，ROS）会积聚，同时当RIP3的表达水平敲低时，胞内ROS的积聚也受到了抑制。但是RIP3如何影响ROS的具体机制仍不清楚。 因为ROS的产生主要在线粒体电子传递链上，而还原型辅酶Ⅰ（NADH）和还原型辅酶Ⅱ（NADPH ...
张丽芳
core
Systematic Entomology, Volume 50, Issue 3, Page 495-518, July 2025.Using genomic data from 285 species across 19 planthopper families, we conducted the first phylogenomic study targeting global planthoppers and inferred their phylogeny with the highest resolution so far. We proposed taxonomic changes including the establishment of a new family Borysthenidae stat. rev. and a new superfamily Meenoploidea superfam.
Junchen Deng +9 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2013 目的比较两种不同方法提取的基因组DNA的纯度、产量和后续实验效果。方法采用酚/氯仿法和盐析法两种方法直接从全血中提取基因组DNA,并采用电泳、PCR和基质支持的激光释放/电离飞行时间质谱分析（MALDI-TOF MS）方法进行检测。结果酚/氯仿法提取的基因组DNA纯度高于盐析法 ...
宋洁云 +4 more
doaj
Study on the microbial community structure, diversity and abundance in water-soil interface sediments on the watershed of three rivers [PDF]
, 2013 为了探讨无机盐分、养分及有机质矿化作用对微生物群落结构、多样性和功能微生物丰度影响，河流水土界面微生物群落结构和碳、氮、硫循环功能微生物种群的演替规律，本论文以九龙江、闽江和瓯江三江流域作为研究区域，并增加温室培养实验，对其中水土界面沉积物样品中的理化参数、微生物群落、多样性和功能微生物丰度进行测定；采用基因文库构建技术、变性梯度凝胶电泳技术（DGGE）和illumina高通量测序技术分析了样品中微生物群落结构及活动规律；利用实时荧光定量PCR（RT-PCR）技术对功能微生物进行定量 ...
李虎
core
Large seeds as a defensive strategy against partial granivory in the Fagaceae
Journal of Ecology, Volume 113, Issue 3, Page 598-607, March 2025.Large seeds dispersed by scatter‐hoarding granivores, such as Fagaceae acorns, tolerate partial damage to achieve higher dispersal efficiency. By demonstrating that seed size itself acts as a defensive trait, the findings provide new insights into seed size diversity and evolution, highlighting how large seeds counterbalance reserve losses to evade ...
Si‐Chong Chen +5 more
wiley +1 more source
Research on Biological Nitrogen Removal by Aerobic Denitrification Process at Low-temperature and the Psychrolerant Mechanism of Functional Bacteria [PDF]
, 2015 本研究针对传统生物脱氮工艺存在能耗大、基建和运行费用高、低温运行效果差等缺陷，采用好氧反硝化工艺处理人工模拟含氮污水，实现好氧条件下同一反应器内全程生物脱氮过程。采用逐步提高曝气时间及曝气强度的方式，以KNO3为氮源，以普通城市污水处理厂的活性污泥为接种泥，实现了好氧反硝化菌的富集及工艺的常温启动。在此基础上，研究了温度对好氧反硝化工艺的性能影响，及低温环境下反应器运行条件的调控效果。此外，针对分离得到的异养硝化-好氧反硝化纯菌的耐冷特性，通过测定其在短期降温过程中ATP含量的变化情况 ...
王语颖
core
European Policy Analysis, Volume 11, Issue 1, Page 114-137, Winter 2025.Abstract In Europe, and specifically in countries bordering the Baltic Sea, preparedness issues have moved up the policy agenda since Russia's 2014 and 2022 invasions of Ukraine. “Ordinary people” are encouraged to prepare for crises—be it through stockpiling food at home, fact‐checking information, or signing up for military training.
Alexandra M. Friede
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2003 目的 探讨石蜡包埋组织中提取DNA应用于PCR反应的影响因素。方法 应用从石蜡包埋组织中提取的DNA ,PCR反应扩增甲状腺髓样癌RET基因第 11外显子 ,分析石蜡包埋组织中提取DNA应用于PCR反应的影响因素。结果 10 .0 μm× 1组织切片组 ,PCR反应循环次数 4 0次组 ,PCR反应模板量 0 .0 5 μg组PCR反应取得高质量目的基因DNA。结论 减少提取组织用量有利于减少抑制PCR物质的量 ,有利于反应的成功 ;PCR反应循环次数一般应大于 4 0次 ...
赵吉生 +6 more
doaj
Conservation Biology, Volume 39, Issue 1, February 2025.Abstract Trade restrictions are often advocated and implemented as measures to protect wild species threatened by overexploitation. However, in some instances, their efficacy has been questioned, notably by governments in the southern African (SADC) region, which tend to favor a sustainable use approach to wildlife management. We conducted a systematic
Christina Hiller +1 more
wiley +1 more source