Results 151 to 160 of about 235,447 (289)

Addendum: Site-directed mutagenesis using Pfu DNA polymerase and T4 DNA ligase

BioTechniques, 2005
doaj   +1 more source

Assembly of a functional replication complex without ATP hydrolysis: a direct interaction of bacteriophage T4 gp45 with T4 DNA polymerase.

, 1993
Michael K. Reddy, Steven E. Weitzel, Peter H. von Hippel   +2 more
openalex   +1 more source

Fabrication of Multimodal Anion Exchange Membrane for Chromatographic Purification of Full Adeno‐Associated Viral Capsids

Advanced Materials Interfaces, EarlyView.
A novel multi‐modal anion exchange membrane (MMAEX) is successfully fabricated for separating empty and full AAV2 capsids. The functionalized membrane has an average pore size of 0.5 µm and exhibits both charge and hydrophobic functionality. A full capsid recovery of 72.9% at 85.3% purity with gradient elution, or 70.3% recovery at 92.2% purity with ...
Xiaolei Hao, Sumith Ranil Wickramasinghe, Xianghong Qian   +2 more
wiley   +1 more source

Fueling chromosomal gene diversification and artificial evolution with CRISPR. [PDF]

Genome Biol
Zhu R, Ren C, Bao Z.
europepmc   +1 more source

Murine DNA polymerase alpha fills gaps to completion in a direct assay. Altered kinetics of de novo DNA synthesis at single nucleotide gaps.

, 1990
Scott Davey, Emanuel A. Faust
openalex   +1 more source

Antimicrobial Multi‐Assay Evaluation of (Al‐Doped ZnO)‐Ag and TiO2‐Ag Nanocoatings: Impact of Direct and Indirect Mechanisms

Advanced Materials Interfaces, EarlyView.
This work explores the antimicrobial action of AZO‐Ag and TiO2‐Ag nanocoatings on plastic, demonstrating good efficacy against highly‐critical antibiotic‐resistant bacteria, yeast, and virus. A multi‐assay approach is here exploited to inspect the contribution of direct and indirect antimicrobial mechanisms, and potential human toxicity is also ...
Damiano Squitieri, Roberta Vitali, Cecilia Bartoleschi, Giulia Lombardini, Margherita Cacaci, Lorenzo Degli Esposti, Camilla Fusacchia, Skerxho Osmani, Luca Gavioli, Francesca Bugli, Daniele Valerini   +10 more
wiley   +1 more source

Identification and application of herbicide-resistant 4-hydroxyphenylpyruvate dioxygenase (HPPD) alleles via directed evolution. [PDF]

Plant Biotechnol J
Zhao B, Wang Y, Zhou M, Liu X, Li H, Shi Z, Guo Y, Li T, Yang F, Wang R, Zhao J, Liu Y.   +11 more
europepmc   +1 more source

Smart Sampling Capsule for Capture and Detection of Enteric Pathogens in the Small Intestine

Advanced Materials Technologies, EarlyView.
An ingestible smart sampling capsule for capturing asymptomatic enteric pathogens at their primary sites of interaction within the small intestine. This capability enables non‐invasive detection of early pathogen colonization within the gut environment prior to fecal shedding, providing a robust approach for pathogen surveillance and microbiome studies.
Akshay Krishnakumar, Devendra Sarnaik, Nicholas L. F. Gallina, Robyn R. McCain, Christa Crain, Arun K. Bhunia, Rahim Rahimi   +6 more
wiley   +1 more source

DNA polymerase kappa is the primary translesion synthesis polymerase for aldehyde ICLs. [PDF]

Nucleic Acids Res
van der Sluijs RV, Verkennis AEE, Hodskinson MR, Barnett J, Cruz VM, Hernandez-Quiles M, Liolios T, Morton SB, Hendrikx A, Bos C, Post H, Millington CL, Rouillon C, Ricci G, Mattiroli F, Williams DM, Altelaar M, Vermeulen M, Patel KJ, Knipscheer P.   +19 more
europepmc   +1 more source

3D‐Printed Unibody Microfluidic Devices for Organ‐on‐Chip Applications

Advanced Materials Technologies, EarlyView.
A practical approach is demonstrated to 3D printing unibody microfluidic Organ‐on‐Chip (OoC) devices using commercial resins and desktop DLP printers. The demonstrated functional devices support 2D monolayers, 3D spheroids, and barrier tissues. With optimised design and post‐processing techniques, leak‐free, biocompatible microchannels and membranes ...
Louis Jun Ye Ong, Surasak Kasetsirikul, Laura A. Milton, Xiaolei Nie, Andrea W. Eiken, James Chirnside, Lucy‐May Gillian Young, Gregory P. Nordin, Yi‐Chin Toh   +8 more
wiley   +1 more source