Proceedings of the National Academy of Sciences of the United States of America, 1974 An RNA-directed DNA polymerase was isolated from the peripheral blood leukocytes of a patient with acute myelomonocytic leukemia by successive purification of a particulate cytoplasmic fraction with endogenous, ribonuclease-sensitive DNA polymerase ...
R. Gallagher +4 more
semanticscholar +1 more source
HIV-1 reverse transcriptase mutations that confer decreased in vitro susceptibility to anti-RT DNA aptamer RT1t49 confer cross resistance to other anti-RT aptamers but not to standard RT inhibitors [PDF]
, 2005 RNA and DNA aptamers specific for HIV-1 reverse transcriptase (RT) can inhibit reverse transcription in vitro. RNA aptamers have been shown to potently block HIV-1 replication in culture.
Fisher, Timothy S +2 more
core +3 more sources
Mechanisms of PARP inhibitor resistance in cancer and insights into the DNA damage response
Genome Medicine, 2018 Editorial summary Inhibitors of poly(ADP-ribose) polymerase (PARPi) have entered the clinic for the treatment of patients with cancers that lack homology-directed DNA repair, but drug resistance remains a clinical hurdle.
Paola Francica, Sven Rottenberg
doaj +1 more source
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol [PDF]
, 2012 Background: Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques.
Liu, Huanting, Naismith, James Henderson
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Amplification of 4–9-kb Human Genomic DNA Flanking a Known Site Using a Panhandle PCR Variant
BioTechniques, 1997 We present a method for the in vitro amplification of >6.0 kb of DNA flanking a known site. This is accomplished by ligating an oligonucleotide to create an inverted repeat of a portion of the known sequence, followed by single-primer polymerase chain ...
Douglas H. Jones, Stanley C. Winistorfer
doaj +1 more source
The ability to form homodimers is essential for RDM1 to function in RNA-directed DNA methylation. [PDF]
PLoS ONE, 2014 RDM1 (RNA-DIRECTED DNA METHYLATION1) is a small plant-specific protein required for RNA-directed DNA methylation (RdDM). RDM1 interacts with RNA polymerase II (Pol II), ARGONAUTE4 (AGO4), and the de novo DNA methyltransferase DOMAINS REARRANGED ...
Taku Sasaki +4 more
doaj +1 more source
alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis [PDF]
, 2004 At the onset of endospore formation in Bacillus subtilis, the DNA binding protein Spo0A activates transcription from two types of promoters. The first type includes the spoIIG and spoIIE promoters, which are used by sigma(A)-RNA polymerase, whereas the ...
Brannigan, J.A., Kumar, A., Moran, C.P.
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Particles with RNA of high molecular weight and RNA-directed DNA polymerase in human brain tumors.
Proceedings of the National Academy of Sciences of the United States of America, 1973 We have previously shown that neoplastic cells of human breast cancers, leukemias, lymphomas, and sarcomas contain particles similar to the viruses that have been established as etiologic agents of these diseases in mice.
W. Cuatico, J. Cho, S. Spiegelman
semanticscholar +1 more source
Isolation and physical properties of the DNA‐directed RNA polymerase from Thermus thermophilus HB8 [PDF]
European Journal of Biochemistry, 1990 The DNA‐directed RNA polymerase from the extremely thermophilic eubacterium Thermus thermophilus HB8 was purified employing a new and rapid method. The subunit pattern of the enzyme, analyzed by SDS gel electrophoresis, was interpreted as: 140 kDa and 170 kDa for β and β′, 40 kDa for α and 92 kDa for ó.
Stephan Wnendt +3 more
openaire +3 more sources
Chimerization of antibodies by isolation of rearranged genomic variable regions by the polymerase chain reaction [PDF]
, 1991 We describe a new method for amplification, by polymerase chain reaction (PCR), of rearranged segments encoding the variable part of light and heavy chains of an antibody (Ab) from the chromosomal DNA of hybridoma cells for the chimerization ofAbs.
Kaluzab, Brigitte +4 more
core +1 more source