Results 171 to 180 of about 1,587,193 (206)

DNA-SIP identification of phenanthrene-degrading bacteria undergoing bioaugmentation and natural attenuation in petroleum-contaminated soil

Chemosphere, 2021
DNA-stable isotope probing (SIP) with 13C labeled phenanthrene (PHE) as substrate was used to identify specific bacterial degraders during natural attenuation (NA) and bioaugmentation (BA) in petroleum contaminated soil. BA, with the addition of a bacterial suspension mixture named GZ, played a significant role in PHE degradation with a higher PHE ...
Liang, Jidong, Gao, Sha, Wu, Zijun, Rijnaarts, Huub H.M., Grotenhuis, Tim   +4 more
openaire   +3 more sources

DNA-SIP reveals an overlooked methanotroph, Crenothrix sp., involved in methane consumption in shallow lake sediments

Science of The Total Environment, 2022
Methanotrophs are the main consumers of methane produced in lake sediments. In shallow lakes suffering from eutrophication, methanogenesis is accelerated by the excess organic carbon input, and thus methanotrophs play a key role in regulating this methane flux as well as carbon cycling.
Yuyin, Yang, Jianfei, Chen, Jennifer, Pratscher, Shuguang, Xie   +3 more
openaire   +2 more sources

Viral DNA SIP: the isotopic composition of virions is correlated with that of the substrate used for host growth

2022
Abstract DNA Stable Isotope Probing is emerging as a powerful tool to study host-virus interactions. Indeed, since all viruses depend on a host for virion production, a link between the isotopic compositions of hosts and the virions they produce is expected.
Vuong Quoc Hoang Ngo, Maximilien Sotomski, Angeline Guenne, Mahendra Mariadassou, Mart Krupovic, François Enault, Ariane Bize   +6 more
openaire   +1 more source

Analysis of microbial utilization of rice straw in paddy soil using a DNA‐SIP approach

Soil Science Society of America Journal, 2020
Abstract Considering the close connection between soil microorganisms with carbon (C) cycling, the aim of this study was to identify the specific bacterial and fungal microbes that assimilate 13 C from incorporated rice straw and explore their dynamics and characteristics ...
Tengfei Guo, Qian Zhang, Chao Ai, Guoqing Liang, Ping He, Qiuliang Lei, Wei Zhou   +6 more
openaire   +1 more source

New insight into the mechanism underlying the effect of biochar on phenanthrene degradation in contaminated soil revealed through DNA-SIP

Journal of Hazardous Materials, 2022
Biochar has been widely used for the remediation of polycyclic aromatic hydrocarbon (PAH)-contaminated soil, but its mechanism of influencing PAH biodegradation remains unclear. Here, DNA-stable isotope probing coupled with high-throughput sequencing was employed to assess its influence on phenanthrene (PHE) degradation, the active PHE-degrading ...
Jiangqiao, Bao, Jibing, Li, Longfei, Jiang, Weiping, Mei, Mengke, Song, Deyin, Huang, Chunling, Luo, Gan, Zhang   +7 more
openaire   +2 more sources

Active Methanotrophs in Two Contrasting North American Peatland Ecosystems Revealed Using DNA-SIP

Microbial Ecology, 2011
The active methanotroph community was investigated in two contrasting North American peatlands, a nutrient-rich sedge fen and nutrient-poor Sphagnum bog using in vitro incubations and (13)C-DNA stable-isotope probing (SIP) to measure methane (CH(4)) oxidation rates and label active microbes followed by fingerprinting and sequencing of bacterial and ...
Varun, Gupta, Kurt A, Smemo, Joseph B, Yavitt, Nathan, Basiliko   +3 more
openaire   +2 more sources

Analysis of Active Methylotrophic Communities: When DNA-SIP Meets High-Throughput Technologies

2016
Methylotrophs are microorganisms ubiquitous in the environment that can metabolize one-carbon (C1) compounds as carbon and/or energy sources. The activity of these prokaryotes impacts biogeochemical cycles within their respective habitats and can determine whether these habitats act as sources or sinks of C1 compounds.
Martin, Taubert, Carolina, Grob, Alexandra M, Howat, Oliver J, Burns, Yin, Chen, Josh D, Neufeld, J Colin, Murrell   +6 more
openaire   +2 more sources

[Ammonia Oxidation in a Neutral Purple Soil Measured by the 13C-DNA-SIP Method].

Huan jing ke xue= Huanjing kexue, 2019
Increasing evidence suggests that ammonia oxidation in acidic soils is primarily catalyzed by ammonia-oxidizing archaea (AOA), while ammonia-oxidizing bacteria (AOB) drive ammonia oxidation in neutral and alkaline soils in which AOA overwhelmingly outnumber AOB.
Tian-Lin, Liu, Zhi-Hui, Wang, Xiao-Juan, Yan, Yong-Peng, Zhao, Zhong-Jun, Jia, Xian-Jun, Jiang   +5 more
openaire   +1 more source

Thiosulfate drives vanadium natural attenuation in oligotrophic mine tailings: Insights from DNA-SIP and metagenomics

Environmental Pollution
Vanadium (V) accumulation in mine tailing ponds represents a persistent contamination source, posing severe risks to the surrounding ecosystems. Microbial V(V) reduction represents a key pathway of V detoxification, immobilization and attenuation.
Miaomiao, Zhang, Huicai, Sun, Youhua, Ren, Kexin, Chen, Geng, Yan, Baoqin, Li, Ying, Huang, Zewen, Tan, Weimin, Sun   +8 more
openaire   +2 more sources

Probing potential microbial coupling of carbon and nitrogen cycling during decomposition of maize residue by 13C-DNA-SIP

Soil Biology and Biochemistry, 2014
Abstract The links between microbial taxa, in situ organic matter decomposition, and coupling of carbon (C) and nitrogen (N) cycles remain unresolved. Here, we used stable isotope probing (SIP) technique to investigate bacterial carbon assimilation and C and N coupling during decomposition of 13C-labeled maize residue in a black soil from Northeast ...
Fenliang Fan, Chang Yin, Yongjun Tang, Zhaojun Li, Alin Song, Steven A. Wakelin, Jun Zou, Yongchao Liang   +7 more
openaire   +1 more source