Results 11 to 20 of about 4,247,719 (263)

DNA stable isotope probing reveals the impact of trophic interactions on bioaugmentation of soils with different pollution histories [PDF]

Microbiome
Background Bioaugmentation is considered a sustainable and cost-effective methodology to recover contaminated environments, but its outcome is highly variable.
Esteban E. Nieto, Stephanie D. Jurburg, Nicole Steinbach, Sabrina Festa, Irma S. Morelli, Bibiana M. Coppotelli, Antonis Chatzinotas   +6 more
doaj   +5 more sources

Evaluating the accuracy of DNA stable isotope probing [PDF]

, 2017
Originality-Significance Statement By combining DNA Stable Isotope Probing (DNA-SIP) with multiplexed high throughput DNA sequencing (HTS-DNA-SIP), it is now possible to identify patterns of isotope incorporation for thousands of microbial taxa.
Youngblut, Nicholas D., Buckley, Daniel H.   +1 more
openaire   +2 more sources

DNA stable-isotope probing

Nature Protocols, 2007
Stable-isotope probing is a method used in microbial ecology that provides a means by which specific functional groups of organisms that incorporate particular substrates are identified without the prerequisite of cultivation. Stable-isotope-labeled carbon (13C) or nitrogen (15N) sources are assimilated into microbial biomass of environmental samples ...
Neufeld, Josh D., Vohra, Jyotsna, Dumont, Marc G., Lueders, Tillmann, Manefield, Mike, Friedrich, Michael W., Murrell, J. Colin   +6 more
openaire   +5 more sources

Applications of DNA-Stable Isotope Probing in Bioremediation Studies

, 2009
DNA-stable isotope probing, a method to identify active microorganisms without the prerequisite of cultivation, has been widely applied in the study of microorganisms involved in the degradation of environmental pollutants. Recent advances and technique considerations in applying DNA-SIP in bioremediation are highlighted.
Chen, Y., Vorha, J., Murrell, J. C.
openaire   +3 more sources

DNA stable‐isotope probing reveals potential key players for microbial decomposition and degradation of diatom‐derived marine particulate matter [PDF]

MicrobiologyOpen, 2020
Microbially mediated decomposition of particulate organic carbon (POC) is a central component of the oceanic carbon cycle, controlling the flux of organic carbon from the surface ocean to the deep ocean.
Ying Liu, Jiasong Fang, Zhongjun Jia, Songze Chen, Li Zhang, Wei Gao   +5 more
doaj   +2 more sources

Integrated 13C-DNA Stable Isotope Probing and Metagenomics Approaches to Identify Bisphenol A Assimilating Microorganisms and Metabolic Pathways in Biofilms [PDF]

Toxics
Bisphenol A (BPA) is a persistent environmental contaminant requiring effective removal strategies. Biofilms offer advantages over conventional activated sludge for refractory compound degradation, yet the specific microorganisms and mechanisms driving ...
Di Wang, Jiayue Sun, Yunian Zhang, Lingjue Yuan, Xia Xu, Yingang Xue, Haohao Sun   +6 more
doaj   +2 more sources

A liquid chromatography - mass spectrometry method to measure ¹³C-isotope enrichment for DNA stable-isotope probing.

Canadian journal of microbiology, 2012
DNA stable-isotope probing (DNA-SIP) is a cultivation-independent technique that makes it possible to associate metabolic function and taxonomic identity in a wide range of terrestrial and aquatic environments. In DNA-SIP, DNA is labeled via the assimilation of a labeled growth substrate that is subsequently used to identify microorganisms involved in ...
Auclair, Julie, Lépine, François, Villemur, Richard   +2 more
openaire   +3 more sources

Technical considerations for the use of ¹⁵ N‐DNA stable‐isotope probing for functional microbial activity in soils

Rapid Communications in Mass Spectrometry, 2005
Abstract Stable‐isotope DNA probing is a culture‐independent technique that may provide a link between function and phylogeny of active microorganisms. The technique has been used in association with 13 C substrates while here we evaluate feasibility and limitations of
Georg, Cadisch, Mingrelia, Espana, Rachel, Causey, Michael, Richter, Eve, Shaw, J Alun W, Morgan, Clive, Rahn, Gary D, Bending   +7 more
openaire   +3 more sources

DNA stable-isotope probing (DNA-SIP). [PDF]

J Vis Exp, 2010
DNA stable-isotope probing (DNA-SIP) is a powerful technique for identifying active microorganisms that assimilate particular carbon substrates and nutrients into cellular biomass. As such, this cultivation-independent technique has been an important methodology for assigning metabolic function to the diverse communities inhabiting a wide range of ...
Dunford EA, Neufeld JD.
europepmc   +4 more sources

DNA-stable isotope probing (DNA-SIP) identifies marine sponge-associated bacteria actively utilizing dissolved organic matter (DOM). [PDF]

Environ Microbiol, 2021
Sponges possess exceptionally diverse associated microbial communities and play a major role in (re)cycling of dissolved organic matter (DOM) in marine ecosystems.
Campana S, Busch K, Hentschel U, Muyzer G, de Goeij JM.   +4 more
europepmc   +2 more sources