Results 151 to 160 of about 2,473,744 (242)

Effects of storage conditions and digestion time on DNA amplification of biting midge (Culicoides) blood meals. [PDF]

Parasit Vectors, 2023
Bellekom B, Bailey A, England M, Langlands Z, Lewis OT, Hackett TD.   +5 more
europepmc   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

R-loops as a trigger for intra- and extrachromosomal DNA amplification in cancer. [PDF]

Front Cell Dev Biol
Kovalenko TF, Abdurazakov A, Antipova NV, Shakhparonov MI, Pavlyukov MS.   +4 more
europepmc   +1 more source

Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics. [PDF]

Nat Commun, 2022
Gavrilov M, Yang JYC, Zou RS, Ma W, Lee CY, Mohapatra S, Kang J, Liao TW, Myong S, Ha T.   +9 more
europepmc   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Ultrafast DNA Amplification Using Microchannel Flow-Through PCR Device. [PDF]

Biosensors (Basel), 2022
Lin YH, Liao XJ, Chang W, Chiou CC.
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

DNA amplification tests at universal pre-admission screening with enhanced precaution strategies for asymptomatic patients with COVID-19. [PDF]

IJID Reg, 2023
Nakamura I, Watanabe Y, Fujita H, Kobayashi T, Watanabe H, Itoi T.   +5 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Rapid Isothermal DNA Amplification in Microchambers Detected by Fluorescence RNA Aptamer Transcription. [PDF]

Diagnostics (Basel)
Yaginuma H, Suzuki R, Akamatsu T, Noji H, Tabata KV.   +4 more
europepmc   +1 more source