Results 151 to 160 of about 107,065 (275)

Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli‐Based Tri‐Selection

Advanced Science, EarlyView.
A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son, Goeun Kim, Jungjoon K. Lee   +2 more
wiley   +1 more source

The FOXC2‐LAMA4 Axis Orchestrates Vasculogenic Mimicry and Immunosuppressive Niche Formation to Drive Metastatic Cascade in Renal Cell Carcinoma

Advanced Science, EarlyView.
Advanced clear cell renal cell carcinoma metastasis is driven by a FOXC2+ tumor subpopulation. These cells execute vasculogenic mimicry for dissemination and secrete LAMA4. LAMA4 engages macrophage ITGA6, activating the STAT6/GATA3 axis to polarize macrophages into a pro‐metastatic, immunosuppressive phenotype.
Jiaxi Yao, Tong Xu, Chengyuan Wang, Chenyuan Wang, Junfeng Xie, Rui Zheng, Ke Wang, Xingzuo Jiang, Zewei Hu, Hongwei Jing, Lin Li, Tao Liu   +11 more
wiley   +1 more source

Neuroprotective Effects of Time‐Restricted Feeding Combined With Different Protein Sources in MPTP‐Induced Parkinson's Disease Mice Model and Its Modulatory Impact on Gut Microbiota Metabolism

Advanced Science, EarlyView.
Time‐restricted feeding (TRF) exerts protein‐dependent neuroprotective effects in an MPTP‐induced Parkinson's disease model. In casein‐fed mice, TRF improves gut barrier integrity and reduces neuroinflammation, possibly via modulation of Allobaculum and BCAAs.
Ting Li, Jian Wu, Sheng‐Yang Zhou, Ming‐An Li, Li‐Ping Zhao, Ao Wang, Yi‐Zhi Song, Wen‐Yan Huang, Lu‐Lu Tan, Chen‐Meng Qiao, Wei‐Jiang Zhao, Chun Cui, Yan‐Qin Shen   +12 more
wiley   +1 more source

Decoding Biodiversity in Baiyangdian Lake: A DNA Barcode Reference Library for Aquatic Insects. [PDF]

Insects
Qiao YJ, Wang ZP, Lv MY, Su PD, Wu T, Xu HF, Li YF, Lin XL, Zhang CH.   +8 more
europepmc   +1 more source

From Natural Discovery to AI‐Guided Design: A Curated Collection of Compact Enhancers for Crop Engineering

Advanced Science, EarlyView.
ABSTRACT Precise transgene‐free gene upregulation remains a challenge in crop biotechnology, as conventional enhancers often exceed CRISPR‐mediated knock‐in size constraints and face regulatory hurdles. Here we establish a foundational cross‐species resource of compact transcriptional enhancers developed via STEM‐seq, a high‐throughput screening ...
Qi Yao, Jin Gao, Keling Wang, Yufeng Liu, Peijin Han, Hong Pan, Xiaofeng Yang, Qianlan Yin, Dating Zhong, Lu Ye, Qi Deng, Lingling Gao, Xiaoyu Tu, Dequan Wang, Yuming Lu   +14 more
wiley   +1 more source

A DNA Barcode Reference Library of Native Seed Plants in Chinese Dryland Regions. [PDF]

Sci Data
Song F, Lian J, Liu ZL, Li XJ, Huang WD, Zhao X, Yan X, Jin L, Jin GZ, Zhao XY, Ge XJ.   +10 more
europepmc   +1 more source

SLC2A3‐Mediated Lactate Metabolism Promotes Lung Cancer Bone Metastasis by Modulating P53 Lactylation and Immune Evasion

Advanced Science, EarlyView.
SLC2A3 derived lactate promotes metastasis through p53 lactylation at K120 and osteoclast differentiation. Pharmacological inhibition of SLC2A3 upregulates PD‐1 expression on CD8+ T cells via lactate induced p53 lactylation, which modulates immune evasion. ABSTRACT Bone metastasis is a devastating consequence of lung cancer.
Yi Ding, Yuying Tian, Wenjie Ren, Xianglin Hu, Mengjuan Li, Bei Liu, Chen chen, Yunhan Lu, Lei Li, Wangjun Yan, Kun Li   +10 more
wiley   +1 more source

Advancing Yeast Identification Using High-Throughput DNA Barcode Data From a Curated Culture Collection. [PDF]

Mol Ecol Resour
Vu D, de Vries M, van den Ende BG, Houbraken J, Nilsson RH, Brankovics B, Hernández‐Restrepo M, Groenewald JZ, Crous PW, Hagen F, Meyer W, Verkley GJM, Groenewald M.   +12 more
europepmc   +1 more source

Structure‐Guided Engineering of a Cas12i Nuclease Unlocks Near‐PAMless Genome Editing

Advanced Science, EarlyView.
CRISPR‐Cas nucleases are limited by PAM requirements, restricting genome accessibility. Structure‐guided engineering of the compact Cas12i nuclease SF01 produced three variants with near‐PAMless, enabling efficient editing at diverse 5'‐NNTN‐3' sites. These nucleases expand the editable portion of the human genome more than fourfold, enabling efficient
Qitong Chen, Hanlin Gou, Chao Xu, Sihan Wang, Huitao Zhang, Minglei Song, Mengge Wang, Xingkun Ji, Xiaofei Wei, Yuanyan Tan, Hehua Quan, Pengyu Luo, Hanyu Shou, Pengpeng Liu, Yafeng Liang, Jian‐Kang Zhu   +15 more
wiley   +1 more source

A DNA Barcode Inventory of Austrian Dragonfly and Damselfly (Insecta: Odonata) Species. [PDF]

Insects
Zangl L, Fischer I, Sittenthaler M, Chovanec A, Gros P, Holzinger W, Kunz G, Lienhard A, Macek O, Mayerhofer C, Mladinić M, Topić M, Schäffer S, Sefc KM, Sturmbauer C, Haring E, Koblmüller S.   +16 more
europepmc   +1 more source