Results 1 to 10 of about 112,962 (287)

A dual-mechanism antimicrobial peptide with antimutagenic activity targets the replisome and induces cell envelope stress [PDF]

mSphere
To combat the growing threat of multidrug-resistant bacteria, we need to develop novel antibiotics with unique modes of action. This study investigates the antibacterial properties of BTP-001 toward Escherichia coli.
Amanda Holstad Singleton, Olaug Elisabeth Torheim Bergum, Jana Scheffold, Synnøve Brandt Ræder, Lilja Brekke Thorfinnsdottir, Lisa Marie Røst, Caroline Krogh Søgaard, Per Bruheim, Marit Otterlei   +8 more
doaj   +2 more sources

Effectiveness of blocking primers and a peptide nucleic acid (PNA) clamp for 18S metabarcoding dietary analysis of herbivorous fish.

PLoS ONE, 2022
The structure of food webs and carbon flow in aquatic ecosystems can be better understood by studying contributing factors such as the diets of herbivorous fish.
Chiho Homma, Daiki Inokuchi, Yohei Nakamura, Wilfredo H Uy, Kouhei Ohnishi, Haruo Yamaguchi, Masao Adachi   +6 more
doaj   +3 more sources

A second DNA binding site on RFC facilitates clamp loading at gapped or nicked DNA

eLife, 2022
Clamp loaders place circular sliding clamp proteins onto DNA so that clamp-binding partner proteins can synthesize, scan, and repair the genome. DNA with nicks or small single-stranded gaps are common clamp-loading targets in DNA repair, yet these ...
Xingchen Liu, Christl Gaubitz, Joshua Pajak, Brian A Kelch   +3 more
doaj   +1 more source

Evolution and origin of sliding clamp in bacteria, archaea and eukarya.

PLoS ONE, 2021
The replication of DNA is an essential process in all domains of life. A protein often involved in replication is the sliding clamp. The sliding clamp encircles the DNA and helps replicative polymerase stay attached to the replication machinery ...
Sandesh Acharya, Amol Dahal, Hitesh Kumar Bhattarai   +2 more
doaj   +2 more sources

Identification of β Clamp-DNA Interaction Regions That Impair the Ability of E. coli to Tolerate Specific Classes of DNA Damage. [PDF]

PLoS ONE, 2016
The E. coli dnaN-encoded β sliding clamp protein plays a pivotal role in managing the actions on DNA of the 5 bacterial DNA polymerases, proteins involved in mismatch repair, as well as several additional proteins involved in DNA replication.
Michael T Nanfara, Vignesh M P Babu, Mohamed A Ghazy, Mark D Sutton   +3 more
doaj   +1 more source

Investigating the composition and recruitment of the mycobacterial ImuA′–ImuB–DnaE2 mutasome

eLife, 2023
A DNA damage-inducible mutagenic gene cassette has been implicated in the emergence of drug resistance in Mycobacterium tuberculosis during anti-tuberculosis (TB) chemotherapy.
Sophia Gessner, Zela Alexandria-Mae Martin, Michael A Reiche, Joana A Santos, Ryan Dinkele, Atondaho Ramudzuli, Neeraj Dhar, Timothy J de Wet, Saber Anoosheh, Dirk M Lang, Jesse Aaron, Teng-Leong Chew, Jennifer Herrmann, Rolf Müller, John D McKinney, Roger Woodgate, Valerie Mizrahi, Česlovas Venclovas, Meindert H Lamers, Digby F Warner   +19 more
doaj   +1 more source

Dynamics of Open DNA Sliding Clamps. [PDF]

PLoS ONE, 2016
A range of enzymes in DNA replication and repair bind to DNA-clamps: torus-shaped proteins that encircle double-stranded DNA and act as mobile tethers.
Aaron J Oakley
doaj   +1 more source

Cryo-EM structures reveal high-resolution mechanism of a DNA polymerase sliding clamp loader

eLife, 2022
Sliding clamps are ring-shaped protein complexes that are integral to the DNA replication machinery of all life. Sliding clamps are opened and installed onto DNA by clamp loader AAA+ ATPase complexes.
Christl Gaubitz, Xingchen Liu, Joshua Pajak, Nicholas P Stone, Janelle A Hayes, Gabriel Demo, Brian A Kelch   +6 more
doaj   +1 more source

Structure-function analysis of the C-clamp of TCF/Pangolin in Wnt/ß-catenin signaling. [PDF]

PLoS ONE, 2014
The evolutionarily conserved Wnt/ß-catenin (Wnt/ß-cat) pathway plays an important role in animal development in metazoans. Many Wnt targets are regulated by members of the TCF/LEF1 (TCF) family of transcription factors.
Aditi J Ravindranath, Ken M Cadigan
doaj   +1 more source

Proficient replication of the yeast genome by a viral DNA polymerase [PDF]

, 2016
DNA replication in eukaryotic cells requires minimally three B-family DNA polymerases: Pol α, Pol δ, and Pol ϵ. Pol δ replicates and matures Okazaki fragments on the lagging strand of the replication fork.
Burgers, Peter M, Stith, Carrie M, Stodola, Joseph L   +2 more
core   +2 more sources