Results 91 to 100 of about 113,396 (329)

The bacterial DNA sliding clamp, β-clamp: structure, interactions, dynamics and drug discovery [PDF]

Cellular and Molecular Life Sciences
AbstractDNA replication is a tightly coordinated event carried out by a multiprotein replication complex. An essential factor in the bacterial replication complex is the ring-shaped DNA sliding clamp, β-clamp, ensuring processive DNA replication and DNA repair through tethering of polymerases and DNA repair proteins to DNA.
Signe Simonsen, Caroline K. Søgaard, Johan G. Olsen, Marit Otterlei, Birthe B. Kragelund   +4 more
openaire   +3 more sources

Contributions of the individual hydrophobic clefts of the Escherichia coli   sliding clamp to clamp loading, DNA replication and clamp recycling [PDF]

Nucleic Acids Research, 2009
The homodimeric Escherichia coli beta sliding clamp contains two hydrophobic clefts with which proteins involved in DNA replication, repair and damage tolerance interact. Deletion of the C-terminal five residues of beta (beta(C)) disrupted both clefts, severely impairing interactions of the clamp with the DnaX clamp loader, as well as the replicative ...
Scouten Ponticelli, Sarah K., Duzen, Jill M., Sutton, Mark D.   +2 more
openaire   +2 more sources

Water skating: How polymerase sliding clamps move on DNA

The FEBS Journal, 2021
Polymerase sliding clamps are ring‐shaped proteins that encircle duplex DNA and hold polymerases to DNA for high processivity during synthesis. The crystal structure of clamp‐DNA complex reveals that the DNA is highly tilted through the clamp with extensive interaction with the clamp inner surface.
Huilin Li, Fengwei Zheng, Mike O’Donnell   +2 more
openaire   +3 more sources

Infusible Extracellular Matrix Biomaterial Enhances Cell‐Specific Pro‐Repair Responses Following Acute Myocardial Infarction

Advanced Healthcare Materials, EarlyView.
We measure the cell‐specific responses of administering infusible ECM (iECM) in acute myocardial infarction (MI) across multiple timepoints. Using single‐nucleus RNA sequencing and spatial transcriptomics, we measure macrophage activation, fibroblast remodeling, increased vascular development, lymphangiogenesis, cardioprotection, and neurogenesis ...
Joshua M. Mesfin, Alexander Chen, Quincy P. Lyons, Michael B. Nguyen, Maria L. Karkanitsa, Justin Yu, Van K. Ninh, Emily Gardner, Elyse G. Wong, Sriya N. Paleti, Julian Cheng, Benjamin D. Bridgelal, Kate E. Reimold, Selena Cao, Connor Uhre, Colin G. Luo, Zhenxing Fu, Kevin R. King, Karen L. Christman   +18 more
wiley   +1 more source

Screening of E. coli β-clamp Inhibitors Revealed that Few Inhibit Helicobacter pylori More Effectively: Structural and Functional Characterization

Antibiotics, 2018
The characteristic of interaction with various enzymes and processivity-promoting nature during DNA replication makes β-clamp an important drug target. Helicobacter pylori (H.
Preeti Pandey, Vijay Verma, Suman Kumar Dhar, Samudrala Gourinath   +3 more
doaj   +1 more source

Sustainable Power Production in a Membrane-less and Mediator-less Wastewater Microbial Fuel Cell [PDF]

, 2009
Microbial fuel cells (MFCs) fed with wastewater are currently considered a feasible strategy for production of renewable electricity at low cost. A membrane-less MFC with biological cathode was built from a compact wastewater treatment reactor.
Aldrovandi, Aba, Giondano, Andrea, Marsili, Enrico, Paganin, Patrizia, Stante, Loredana, Tabacchioni, Silvia   +5 more
core   +1 more source

Decellularized Extracellular Matrix (dECM) in Tendon Regeneration: A Comprehensive Review

Advanced Healthcare Materials, EarlyView.
Decellularized Extracellular Matrix (dECM) offers a promising solution by replicating the native tendon microenvironment and promoting regeneration. This review highlights advances in the decellularization methods, as well as their integration with emerging technologies and translational progress in tendon tissue engineering.
Kumaresan Sakthiabirami, Yeonwoo Park, Seong Kyeong Jo, Min Hee Kim, Jae Hwang Song   +4 more
wiley   +1 more source

Tunable Bioresorbable Scaffolds With Marine Sulfated Polysaccharides for Small‐Caliber Vascular Grafts: A Multi‐Layered Strategy Combining Electrospinning and 4‐Axis Printing

Advanced Healthcare Materials, EarlyView.
A multilayered small‐caliber vascular scaffold combining electrospinning and 4‐axis printing is developed and biofunctionalized with marine sulfated polysaccharides from Holothuria tubulosa. The resulting construct exhibits enhanced hemocompatibility, tunable mechanical properties, and supports endothelial and smooth muscle cell adhesion and ...
Gabriele Obino, Alberto Sensini, Tim ten Brink, Gabriele Nieddu, Tristan Bodet, Giovanni Andrea Deiana, Martijn van Griensven, Marilena Formato, Antonio J. Lepedda, Lorenzo Moroni   +9 more
wiley   +1 more source

The elemental mechanism of transcriptional pausing. [PDF]

, 2019
Transcriptional pausing underlies regulation of cellular RNA biogenesis. A consensus pause sequence that acts on RNA polymerases (RNAPs) from bacteria to mammals halts RNAP in an elemental paused state from which longer-lived pauses can arise.
Chua, Xien Yu, Landick, Robert, Mishanina, Tatiana V, Mooney, Rachel Anne, Nayak, Dhananjaya, Saba, Jason, Windgassen, Tricia A   +6 more
core   +1 more source

The Mre11-Rad50-Nbs1 complex mediates activation of TopBP1 by ATM [PDF]

, 2009
The activation of ATR-ATRIP in response to double-stranded DNA breaks (DSBs) depends upon ATM in human cells and Xenopus egg extracts. One important aspect of this dependency involves regulation of TopBP1 by ATM.
Abraham R. T., Akiko Kumagai, Andrej Shevchenko, Anna Shevchenko, Bakkenist C. J., Byun T. S., Caldecott K. W., Chapman J. R., Cimprich K. A., Cortez D., Costanzo V., Costanzo V., Cuadrado M., Daniel J. Lew, Delacroix S., Garcia V., Glover J. N., Goldberg M., Guo Z., Hae Yong Yoo, Hashimoto Y., Jazayeri A., Kobayashi T., Kumagai A., Kumagai A., Kumagai A., Kumagai A., Lee J., Lee J. H., Liu Q., Melander F., Mordes D. A., Morishima K., Myers J. S., Nyberg K. A., Parrilla-Castellar E. R., Perry J. A., Qin X. F., Rupnik A., Sancar A., Shevchenko A., Shiloh Y., Spycher C., Stewart G. S., Walter J., Waridel P., William G. Dunphy, Williams R. S., Wu L., Yoo H. Y., Yoo H. Y., Yoo H. Y., Yoo H. Y., You Z., You Z., Zhao H., Zou L.   +56 more
core   +2 more sources