Results 111 to 120 of about 113,396 (329)

Small molecules targeting the eubacterial β-sliding clamp discovered by combined in silico and in vitro screening approaches

Journal of Enzyme Inhibition and Medicinal Chemistry
Antibiotic resistance stands as the foremost post-pandemic threat to public health. The urgent need for new, effective antibacterial treatments is evident.
Alessia Caputo, Gian Marco Elisi, Elisabetta Levati, Giulia Barotti, Sara Sartini, Jerome Wagner, Dominique Y. Burnouf, Simone Ottonello, Silvia Rivara, Barbara Montanini   +9 more
doaj   +1 more source

VirB, a transcriptional activator of virulence in Shigella flexneri, uses CTP as a cofactor

Communications Biology, 2023
VirB is a transcriptional activator of virulence in the gram-negative bacterium Shigella flexneri encoded by the large invasion plasmid, pINV. It counteracts the transcriptional silencing by the nucleoid structuring protein, H-NS.
Hammam Antar, Stephan Gruber
doaj   +1 more source

Cellular localisation of the clamp protein during DNA replication [PDF]

FEMS Microbiology Letters, 2002
The beta subunit of Escherichia coli DNA polymerase III holoenzyme was fused to the green fluorescent protein GFP. The gene fusion under the control of the heterologous lac promoter was used to replace the wild-type allele in the chromosome. The formation of GFP-beta fluorescent foci in GFP-beta expressing cells required DNA replication and their ...
Kritaya, Kongsuwan, Brian P, Dalrymple, Gene, Wijffels, Phillip A, Jennings   +3 more
openaire   +2 more sources

An Injection‐Molded Modified Silicone Rubber for Cancer‐on‐Chip Applications

Advanced Materials Technologies, Volume 10, Issue 6, March 18, 2025.
This study describes the characterization and application of an injection‐molded functionalized PDMS fluidic insert for the commercially available Micronit system. Modified PDMS can accommodate cell culture and is able to covalently bind proteins for long‐lasting coatings under flow‐conditions.
Ben J. Haspels, Frederikus Bakker, Lucas J. A. M. Beckers, Roland Kanaar, Koen C. Kriege, Susanne M. Valster, Roland C. M. Vulders, Maayke M. P. Kuijten   +7 more
wiley   +1 more source

Versatile Control System for Automated Single-Molecule Optical Tweezers Investigations [PDF]

, 2010
We present a versatile control system to automate single-molecule biophysics experiments. This method combines low-level controls into various functional, user-configurable modules, which can be scripted in a domain-specific instruction language.
Richard C. Yeh, Steven J. Koch
core   +1 more source

Femtosecond‐Laser‐Fabricated 3D CYTOP Microfluidic Chips Enabling Super‐Resolution Live Imaging of Cell Behavior in Confined Microspaces

Advanced Materials Technologies, EarlyView.
Using two‐photon polymerization of SU‐8 together with mold processing allowed the creation of defect‐free, flexible 3D microfluidic structures with sub‐micrometer precision in CYTOP. The fabricated CYTOP microfluidic chips enabled super‐resolution imaging of cancer cells in the microchannels, clearly visualizing previously undetectable fine structural ...
Koji Sugioka, Mirai Hanzawa, Kotaro Obata, Sota Matsumoto, Masatoshi Takagi, Asako Sakaue‐Sawano, Asako Tosaki, Satoshi Shimozono, Felix Sima, Hiroyuki Kawano, Takuro Tojima, Daisuke Miyashiro, Kazunari Ozasa, Yasutaka Hanada, Godai Miyaji, Akihiko Nakano, Atsushi Miyawaki   +16 more
wiley   +1 more source

The properties of Msh2-Msh6 ATP binding mutants suggest a signal amplification mechanism in DNA mismatch repair. [PDF]

, 2018
DNA mismatch repair (MMR) corrects mispaired DNA bases and small insertion/deletion loops generated by DNA replication errors. After binding a mispair, the eukaryotic mispair recognition complex Msh2-Msh6 binds ATP in both of its nucleotide-binding sites,
Graham, William J, Kolodner, Richard D, Putnam, Christopher D   +2 more
core  

Circular RNA PTPN4 Contributes to Blood‐Brain Barrier Disruption during Early Epileptogenesis

Advanced Science, EarlyView.
Epileptic condition induces CircPTPN4 upregulation, which promotes ECE‐1 expression through competitive sequestration of miR‐145a‐5p. The elevated ECE‐1 catalyzes the ET‐1 production, leading to p38/MAPK pathway activation and subsequent downregulation of tight junction protein expression. This cascade results in increased BBB permeability and enhanced
Jiurong Yang, Yang Hu, Feiyu Wang, Xintao Peng, Honggang Qi, Yuanyuan Yao, Canyu Zhang, Lijie Zhou, Xuemei Liang, Kang Xu, Cong Zhang, Aifeng Zhang, Chen Chen, Yu Zeng, Chenchen Zhang, Guangming Gan, Xinjian Zhu   +16 more
wiley   +1 more source

Single-molecule studies contrast ordered DNA replication with stochastic translesion synthesis

eLife, 2017
High fidelity replicative DNA polymerases are unable to synthesize past DNA adducts that result from diverse chemicals, reactive oxygen species or UV light.
Gengjing Zhao, Emma S Gleave, Meindert Hugo Lamers   +2 more
doaj   +1 more source

Template-directed biopolymerization: tape-copying Turing machines

, 2012
DNA, RNA and proteins are among the most important macromolecules in a living cell. These molecules are polymerized by molecular machines. These natural nano-machines polymerize such macromolecules, adding one monomer at a time, using another linear ...
Chowdhury, Debashish, Sharma, Ajeet K.
core   +1 more source