Results 311 to 320 of about 113,396 (329)
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Sliding Clamps of DNA Polymerases
Journal of Molecular Biology, 1993The determination of the structure of the processivity factor (beta subunit) of Escherichia coli DNA polymerase III holoenzyme showed that this protein acts to clamp the polymerase onto DNA by forming a closed circular structure that can encircle duplex DNA (X.-P. Kong, R. Onrust, M. O'Donnell & J. Kuriyan. (1992). Cell, 69, 425-437). In this review we
J, Kuriyan, M, O'Donnell
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Structural analysis of a eukaryotic sliding DNA clamp–clamp loader complex
Nature, 2004Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on DNA polymerases. Here we report the crystal structure of the five-protein clamp loader complex (replication factor-C, RFC) of the yeast Saccharomyces cerevisiae, bound to the sliding clamp (proliferating cell nuclear antigen, PCNA).
Gregory D, Bowman +2 more
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The PCNA–RFC Families of DNA Clamps and Clamp Loaders
2004The proliferating cell nuclear antigen PCNA functions at multiple levels in directing DNA metabolic pathways. Unbound to DNA, PCNA promotes localization of replication factors with a consensus PCNA-binding domain to replication factories. When bound to DNA, PCNA organizes various proteins involved in DNA replication, DNA repair, DNA modification, and ...
Jerzy, Majka, Peter M J, Burgers
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Improved DNA Clamps by Stacking to Adjacent Nucleobases
Helvetica Chimica Acta, 2012AbstractThree or four aromatic rings interconnected by acetylene bridges form a stiff conjugated system with sufficient conformational freedom to make it useful to link together the two strands of a DNA clamp. Upon targeting a ssDNA, the conformational flexibility allows better stacking of the linker to the underlying non‐planar base triplet in the ...
Fatthalla, M.I. +1 more
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The clamp-loading complex for processive DNA replication
Nature Structural & Molecular Biology, 2004DNA polymerase requires two processing factors, sliding clamps and clamp loaders, to direct rapid and accurate duplication of genomic DNA. In eukaryotes, proliferating cell nuclear antigen (PCNA), the ring-shaped sliding clamp, encircles double-stranded DNA within its central hole and tethers the DNA polymerases onto DNA.
Tomoko, Miyata +5 more
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Functions of Multiple Clamp and Clamp-Loader Complexes in Eukaryotic DNA Replication
2017Proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) were identified in the late 1980s as essential factors for replication of simian virus 40 DNA in human cells, by reconstitution of the reaction in vitro. Initially, they were only thought to be involved in the elongation stage of DNA replication.
Eiji, Ohashi, Toshiki, Tsurimoto
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An Alternative Clamp Loading Pathway via the T4 Clamp Loader gp44/62−DNA Complex
Biochemistry, 2006In bacteriophage T4, a clamp loading pathway that utilizes the T4 clamp loader (gp44/62) and ATP hydrolysis initially to form a complex with the clamp (gp45) has been demonstrated, followed by interaction with DNA and closing of the clamp. However, the recent observation that gp45 exists as an opened form in solution raises the possibility of other ...
Zhihao, Zhuang +2 more
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PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated DNA
Genes to Cells, 2002AbstractBackground: Proliferating cell nuclear antigen (PCNA) is a ring‐shaped protein known as a processivity factor of DNA polymerase δ. In addition to this role, PCNA interacts with a number of other proteins to increase their local concentration at replicated DNA sites. DNA cytosine methyltransferase 1 (Dnmt1), which preserves epigenetic signals by
Tetsuo, Iida +6 more
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MukB acts as a macromolecular clamp in DNA condensation
Nature Structural & Molecular Biology, 2008Correct folding of the chromosome into its highly ordered structure requires the action of condensins. The multisubunit condensins are highly conserved from bacteria to humans, and at their core they contain the characteristic V-shaped dimer of structural maintenance of chromosome proteins.
Yuanbo, Cui +2 more
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A Patch-Clamp ASIC for Nanopore-Based DNA Analysis
IEEE Transactions on Biomedical Circuits and Systems, 2013In this paper, a fully integrated high-sensitivity patch-clamp system is proposed for single-molecule deoxyribonucleic acid (DNA) analysis using a nanopore sensor. This system is composed of two main blocks for amplification and compensation. The amplification block is composed of three stages: 1) a headstage, 2) a voltage-gain difference amplifier ...
Jungsuk, Kim +3 more
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