Results 101 to 110 of about 888,680 (335)

Dual inhibition of DNA-PK and Polϴ boosts precision of diverse prime editing systems

Nature Communications
Prime editing is a genome engineering tool that allows installation of various small edits with high precision. However, prime editing efficiency and purity can vary widely across different edits, genomic targets, and cell types.
Louis C. Dacquay, Panagiotis Antoniou, Astrid Mentani, Niklas Selfjord, Hanna Mårtensson, Pei-Pei Hsieh, Salman Mustfa, George Thom, Sandra Wimberger, Mike Firth, Nina Akrap, Marcello Maresca, Martin Peterka   +12 more
doaj   +1 more source

Genomic Identification of RNA Editing Through Integrating Omics Datasets and the Clinical Relevance in Hepatocellular Carcinoma

Frontiers in Oncology, 2020
RNA editing is a widespread post-transcriptional mechanism to introduce single nucleotide changes to RNA in human cancers. Here, we characterized the global RNA editing profiles of 373 hepatocellular carcinoma (HCC) and 50 adjacent normal liver samples ...
Juan Chen, Lu Wang, Fangbin Wang, Jian Liu, Jian Liu, Zhenyu Bai   +5 more
doaj   +1 more source

Cryptography in the DNA of living cells enabled by multi-site base editing [PDF]

, 2023
Verena Volf, Simon Zhang, Karen M. Song, Sharon Qian, Fei Chen, George M. Church   +5 more
openalex   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Transfer of mitochondrial DNA into the nuclear genome during induced DNA breaks

Nature Communications
Mitochondria serve as the cellular powerhouse, and their distinct DNA makes them a prospective target for gene editing to treat genetic disorders. However, the impact of genome editing on mitochondrial DNA (mtDNA) stability remains a mystery.
Jinchun Wu, Yang Liu, Liqiong Ou, Tingting Gan, Zhengrong Zhangding, Shaopeng Yuan, Xinyi Liu, Mengzhu Liu, Jiasheng Li, Jianhang Yin, Changchang Xin, Ye Tian, Jiazhi Hu   +12 more
doaj   +1 more source

Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems. [PDF]

PLoS ONE, 2015
Single-stranded DNA oligonucleotides (ssODNs) can direct the repair of a single base mutation in human genes. While the regulation of this gene editing reaction has been partially elucidated, the low frequency with which repair occurs has hampered ...
Pawel Bialk, Natalia Rivera-Torres, Bryan Strouse, Eric B Kmiec   +3 more
doaj   +1 more source

Plasma extrachromosomal circular DNA as a biomarker in EGFR‐targeted therapy of non‐small cell lung cancer

Molecular Oncology, EarlyView.
Detection of extrachromosomal circular DNA (eccDNA) in plasma samples from EGFR‐mutated non‐small cell lung cancer patients. Plasma was collected before and during treatment with the EGFR‐tyrosine kinase inhibitor osimertinib. Plasma eccDNA was detected in all cancer samples, and the presence of the EGFR gene on eccDNA serves as a potential biomarker ...
Simone Stensgaard, Sarmad Mehmood, Eva Boysen Fynboe Ebert, Peter Meldgaard, Anindya Dutta, Boe S. Sorensen   +5 more
wiley   +1 more source

ApoE gene therapy: an overview and update [PDF]

, 2005
Atherosclerosis remains the leading cause of death in industrialized societies. Apolipoprotein E (ApoE) is an attractive candidate to treat hypercholesterolemia and coronary heart disease, as it is a circulating protein with pleiotropic ...
Owen, J.S.
core  

Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes [PDF]

, 2018
The Cas9 endonuclease can be programmed by guide RNA to introduce sequence-specific breaks in genomic DNA. Thus, Cas9-based approaches present a range of novel options for genome manipulation and precision editing. African trypanosomes are parasites that
A Paix, B Raz, BAA Romagnoli, CE Boothroyd, CE Clayton, D Horn, D Peng, DL Jones, E Wirtz, EL Ivanov, FE Graf, FE Graf, IM Vincent, J Song, JG Doench, JH Hu, JS Chen, L Glover, L Glover, L Glover, L Glover, L Sollelis, LC Soares Medeiros, LF Lye, M Berriman, M Jinek, M Meissner, MA Marchetti, MC Field, N Baker, N Lander, N Lander, PA Marin, PD Hsu, R Barrangou, S Alsford, S Alsford, S Dean, SM Duncan, SM Sidik, T Beneke, WW Zhang, WW Zhang, X Ren, Y Fu, Y Fu, Y Gao   +46 more
core   +2 more sources

Cis‐regulatory and long noncoding RNA alterations in breast cancer – current insights, biomarker utility, and the critical need for functional validation

Molecular Oncology, EarlyView.
The noncoding region of the genome plays a key role in regulating gene expression, and mutations within these regions are capable of altering it. Researchers have identified multiple functional noncoding mutations associated with increased cancer risk in the genome of breast cancer patients.
Arnau Cuy Saqués, Aracele Martinez‐Mendez, John Crown, Alex Eustace   +3 more
wiley   +1 more source