Proteins that contain a functional Z-DNA-binding domain localize to cytoplasmic stress granules [PDF]
, 2013 Long double-stranded RNA may undergo hyper-editing by adenosine deaminases that act on RNA (ADARs), where up to 50% of adenosine residues may be converted to inosine.
Ng, Siew Kit +3 more
core +1 more source
Mitochondrial DNA Base Editing: Good Editing Things Still Come in Small Packages [PDF]
Molecular Cell, 2020 The collaborative work of two HHMI groups, one at the University of Washington and the other at the Broad Institute of MIT and Harvard, led to the development of a novel molecular tool to edit single bases in the mtDNA (Mok et al., 2020).
Sandra R, Bacman, Carlos T, Moraes
openaire +2 more sources
Prime Editing: Mechanistic Insights and DNA Repair Modulation
CellsPrime editing is a genome editing technique that allows precise modifications of cellular DNA without relying on donor DNA templates. Recently, several different prime editor proteins have been published in the literature, relying on single- or double ...
Astrid Mentani +2 more
doaj +1 more source
Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response
Cell Reports, 2020 Summary: The advent of base editors (BEs) holds great potential for correcting pathogenic-related point mutations to treat relevant diseases. However, Cas9 nickase (nCas9)-derived BEs lead to DNA double-strand breaks, which can trigger unwanted DNA ...
Xiao Wang +17 more
doaj +1 more source
Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
, 2015 Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas +3 more
core +2 more sources
Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators. [PDF]
PLoS ONE, 2018 Prokaryotic Argonaute proteins (pAgos) have been proposed as an alternative to the CRISPR/Cas9 platform for gene editing. Although Argonaute from Natronobacterium gregoryi (NgAgo) was recently shown unable to cleave genomic DNA in mammalian cells, the ...
Henriette O'Geen +4 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2022 Precise genome editing of human pluripotent stem cells (hPSCs) is crucial not only for basic science but also for biomedical applications such as ex vivo stem cell therapy and genetic disease modeling.
Ju-Chan Park +7 more
doaj +1 more source
Promoter keyholes enable specific and persistent multi-gene expression programs in primary T cells without genome modification [PDF]
, 2020 Non-invasive epigenome editing is a promising strategy for engineering gene expression programs, yet potency, specificity, and persistence remain challenging.
Acosta, Reyes +25 more
core
Herpesviral lytic gene functions render the viral genome susceptible to novel editing by CRISPR/Cas9
eLife, 2019 Herpes simplex virus (HSV) establishes lifelong latent infection and can cause serious human disease, but current antiviral therapies target lytic but not latent infection.
Hyung Suk Oh +6 more
doaj +1 more source
Nanodelivery of a functional membrane receptor to manipulate cellular phenotype. [PDF]
, 2018 Modification of membrane receptor makeup is one of the most efficient ways to control input-output signals but is usually achieved by expressing DNA or RNA-encoded proteins or by using other genome-editing methods, which can be technically challenging ...
Baikoghli, Mo +7 more
core +2 more sources