Results 111 to 120 of about 55,108 (306)

Dissecting DNA repair in adult high grade gliomas for patient stratification in the post-genomic era

, 2014
Deregulation of multiple DNA repair pathways may contribute to aggressive biology and therapy resistance in gliomas. We evaluated transcript levels of 157 genes involved in DNA repair in an adult glioblastoma Test set (n=191) and validated in ‘The Cancer
Agarwal, D, Auer, Dorothee T, Lourdusamy, Anbarasu, Chan, Stephen, Ball, G, Lakhani, R, Grundy, Richard, Lourdusamy, A, Ball, Graham, Scott, Ian S, Abdel-Fatah, Tarek M.A., Auer, DT, Scott, IS, Lakhani, Ravi, Grundy, R, Walker, David, Agarwal, Devika, Abdel-Fatah, TMA, Chan, S, Scott, Ian S., Madhusudan, Srinivasan, Perry, C, Abdel-Fatah, Tarek MA, Madhusudan, S, Walker, D, Perry, Christina, Auer, Dorothee T.   +26 more
core   +1 more source

Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes

Nature Communications, 2016
Most adult mammalian cells prefer to repair double-strand DNA breaks though the classical nonhomologous end-joining pathway. Here the authors present evidence that a nascent RNA transcript can serve as a template to facilitate error-free repair.
Anirban Chakraborty, Nisha Tapryal, Tatiana Venkova, Nobuo Horikoshi, Raj K. Pandita, Altaf H. Sarker, Partha S. Sarkar, Tej K. Pandita, Tapas K. Hazra   +8 more
doaj   +1 more source

Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania

mSphere, 2019
CRISPR-Cas9 genome editing relies on an efficient double-strand DNA break (DSB) and repair. Contrary to mammalian cells, the protozoan parasite Leishmania lacks the most efficient nonhomologous end-joining pathway and uses microhomology-mediated end ...
Wen-Wei Zhang, Greg Matlashewski
doaj   +1 more source

Infusible Extracellular Matrix Biomaterial Enhances Cell‐Specific Pro‐Repair Responses Following Acute Myocardial Infarction

Advanced Healthcare Materials, EarlyView.
We measure the cell‐specific responses of administering infusible ECM (iECM) in acute myocardial infarction (MI) across multiple timepoints. Using single‐nucleus RNA sequencing and spatial transcriptomics, we measure macrophage activation, fibroblast remodeling, increased vascular development, lymphangiogenesis, cardioprotection, and neurogenesis ...
Joshua M. Mesfin, Alexander Chen, Quincy P. Lyons, Michael B. Nguyen, Maria L. Karkanitsa, Justin Yu, Van K. Ninh, Emily Gardner, Elyse G. Wong, Sriya N. Paleti, Julian Cheng, Benjamin D. Bridgelal, Kate E. Reimold, Selena Cao, Connor Uhre, Colin G. Luo, Zhenxing Fu, Kevin R. King, Karen L. Christman   +18 more
wiley   +1 more source

A role for alternative end-joining factors in homologous recombination and genome editing in Chinese hamster ovary cells.

, 2019
CRISPR technologies greatly foster genome editing in mammalian cells through site-directed DNA double strand breaks (DSBs). However, precise editing outcomes, as mediated by homologous recombination (HR) repair, are typically infrequent and outnumbered ...
Bosshard, S., Duroy, P.O., Mermod, N.
core   +1 more source

Regulation of protein kinase B (PKB/Akt) by DNA-dependent protein kinase (DNA-PK) under physiological conditions [PDF]

, 2010
The serine/threonine protein kinase B (PKB/Akt) is a downstream effector of phosphatidylinositol 3-kinase (PI3K) and a major regulator of a variety of cellular processes, including metabolism, transcription, survival, proliferation, and growth.
Sürücü, Banu
core   +1 more source

DNA Repair Capacity for Personalizing Risk and Treatment Response − Assay Development and Optimization in Human Peripheral Blood Mononuclear Cells (PBMCs)

, 2022
DNA repair capacity (DRC) is the ability of a cell to repair DNA damage. Differential DRC plays an important role in human disease, including lung and other cancers. Measuring DRC could aid in translational disease research and in personalizing treatment.
Sears, Catherine R., Zhou, Huaxin, Huaxin Zhou, Nawar Al Nasrallah, Patricia A. Smith, Smith, Patricia A., Al Nasrallah, Nawar, Catherine R. Sears   +7 more
core   +1 more source

Multidimensional Cellular Micro‐Compartments to Model Invasive Lobular Carcinoma Dormancy

Advanced Healthcare Materials, EarlyView.
Invasive lobular carcinoma (ILC) is an understudied subtype of breast cancer that is susceptible to late recurrences. In this study, micro‐compartmentalization techniques spanning multiple dimensions, including 2D, pseudo‐3D, and 3D, are integrated to uncover the mechanisms underlying ILC dormancy, revealing the central role of p27Kip1.
Xilal Y. Rima, Sarmila Majumder, Divya S. Patel, Chunyu Hu, Hong Li, Xin Huang, Kim Truc Nguyen, Jacob Doon‐Ralls, Chiranth K. Nagaraj, Mangesh D. Hade, Setty M. Magaña, Eswar Shankar, Xiaoli Zhang, Daniel G. Stover, Bhuvaneswari Ramaswamy, Eduardo Reátegui   +15 more
wiley   +1 more source

Transcription influences repair-induced DNA methylation

, 2008
This work is aimed at the dissection of the molecular mechanism(s) linking DNA damage and gene silencing. To this end, we have developed a genetic system that allows a rapid assessment of homologous-directed repair (HR) of an unique DNA double strand ...
Morano, Annalisa
core  

Mutations in Two Ku Homologs Define a DNA End-Joining Repair Pathway in Saccharomyces Cerevisiae

, 1996
DNA double-strand break (DSB) repair in mammalian cells is dependent on the Ku DNA binding protein complex. However, the mechanism of Ku-mediated repair is not understood.
Weaver, David T., Jin, Shengfang, Shannon, Katie, Milne, George Todd   +3 more
core   +1 more source