Microhomology-mediated end joining induces hypermutagenesis at breakpoint junctions. [PDF]
PLoS Genetics, 2017 Microhomology (MH) flanking a DNA double-strand break (DSB) drives chromosomal rearrangements but its role in mutagenesis has not yet been analyzed. Here we determined the mutation frequency of a URA3 reporter gene placed at multiple locations distal to ...
Supriya Sinha +7 more
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Robust activation of microhomology-mediated end joining for precision gene editing applications. [PDF]
PLoS Genetics, 2018 One key problem in precision genome editing is the unpredictable plurality of sequence outcomes at the site of targeted DNA double stranded breaks (DSBs).
Hirotaka Ata +9 more
doaj +3 more sources
Recurrent deletions in clonal hematopoiesis are driven by microhomology-mediated end joining [PDF]
Nature Communications, 2021 The mutational mechanisms that produce insertions and deletions that lead to clonal hematopoiesis are poorly understood. Here the authors show evidence that frequent deletions that are relevant to myeloid malignancies could be produced by PARP1-dependent
Tzah Feldman +13 more
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Translesion synthesis and microhomology-mediated end-joining repair in trypanosomatids. [PDF]
PLoS Neglected Tropical DiseasesTrypanosomatid parasites and the diseases they cause affect more than 30 million people annually worldwide. To develop treatments for these diseases, it is critical to understand how trypanosomatid biology protects the parasite, so that these mechanisms ...
Lea Drogalis Beckham +3 more
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Structural basis for Polθ-helicase DNA binding and microhomology-mediated end-joining [PDF]
Nature CommunicationsDNA double-strand breaks (DSBs) present a critical threat to genomic integrity, often precipitating genomic instability and oncogenesis. Repair of DSBs predominantly occurs through homologous recombination (HR) and non-homologous end joining (NHEJ).
Fumiaki Ito +5 more
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Polθ promotes the repair of 5′-DNA-protein crosslinks by microhomology-mediated end-joining [PDF]
Cell Reports, 2021 Summary: DNA polymerase θ (Polθ) confers resistance to chemotherapy agents that cause DNA-protein crosslinks (DPCs) at double-strand breaks (DSBs), such as topoisomerase inhibitors. This suggests Polθ might facilitate DPC repair by microhomology-mediated
Gurushankar Chandramouly +11 more
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Molecular basis of microhomology-mediated end-joining by purified full-length Polθ [PDF]
Nature Communications, 2019 DNA polymerase θ is a polymerase-helicase essential for microhomology-mediated end-joining (MMEJ) or alternative end-joining of DNA. Here the authors use biochemical and biophysical methods to reveal how full-length human DNA polymerase θ performs MMEJ ...
Samuel J. Black +18 more
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In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joining [PDF]
Life Science Alliance, 2020 This study provides a new method that enables the labelling of knockout cells with fluorescent protein through microhomology-mediated end joining (MMEJ)–based knock-in. Gene knockout is important for understanding gene function and genetic disorders. The
Shota Katayama, Kota Sato, Toru Nakazawa
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Optimizing CRISPR precision in mouse embryos via microhomology-mediated end joining-dominant targeting [PDF]
Communications BiologyCRISPR/Cas9 technology enables efficient gene editing in mice, but its reliance on non-homologous end joining often leads to unpredictable and mosaic mutations in founder (F0) animals.
Khanui Lkhagvadorj +8 more
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Endogenous CRISPR-assisted microhomology-mediated end joining enables rapid genome editing in Zymomonas mobilis [PDF]
Biotechnology for Biofuels, 2021 Background Zymomonas mobilis is a natural ethanologen with many desirable characteristics, making it an ideal platform for future biorefineries. Recently, an endogenous CRISPR-based genome editing tool has been developed for this species.
Xin Sui +8 more
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