Results 31 to 40 of about 42,496 (156)

Mitotic microhomology-mediated break-induced replication promotes chromoanasynthesis [PDF]

Nature Communications
Chromoanasynthesis is a form of complex chromosomal rearrangement (CCR) commonly detected in cancers and congenital disorders, but the mechanism underlying its generation remain elusive. Here we develop a single-molecule long-read DNA sequencing approach
Greg H. P. Ngo, Kez Cleal, Sara Seifan, Vanda Miklos, Szymon A. Barwacz, Brian L. Ruis, Siamak A. Kamranvar, Julia W. Grimstead, Ying Liu, Eric A. Hendrickson, Duncan M. Baird   +10 more
doaj   +2 more sources

Comparison of Multiple Strategies for Precision Transgene Knock-In in Gallus gallus Genome via Microhomology-Mediated End Joining. [PDF]

Int J Mol Sci, 2023
Precision exogenous gene knock-in is an attractive field for transgenic Gallus gallus (chicken) generation. In this article, we constructed multiple Precise Integration into Target Chromosome (PITCh) plasmid systems mediated by microhomology-mediated end-
Wang L, Sun J, Liu Z, Zheng Q, Wang G.
europepmc   +2 more sources

CRISPR/Cas-based precision genome editing via microhomology-mediated end joining. [PDF]

Plant Biotechnol J, 2021
SummaryGene editing and/or allele introgression with absolute precision and control appear to be the ultimate goals of genetic engineering. Precision genome editing in plants has been developed through various approaches, including oligonucleotide‐directed mutagenesis (ODM), base editing, prime editing and especially homologous recombination (HR)‐based
Van Vu T, Thi Hai Doan D, Kim J, Sung YW, Thi Tran M, Song YJ, Das S, Kim JY.   +7 more
europepmc   +4 more sources

PLK1 targets CtIP to promote microhomology-mediated end joining. [PDF]

Nucleic Acids Res, 2018
Proper DNA double-strand break (DSB) repair is essential for maintaining genome integrity. Microhomology-mediated end joining (MMEJ) is an error-prone repair mechanism, which introduces mutations at break sites and contributes to chromosomal translocations and telomere fusions, thus driving carcinogenesis.
Wang H, Qiu Z, Liu B, Wu Y, Ren J, Liu Y, Zhao Y, Wang Y, Hao S, Li Z, Peng B, Xu X.   +11 more
europepmc   +3 more sources

A comparison of DNA repair pathways to achieve a site-specific gene modification of the Bruton's tyrosine kinase gene

Molecular Therapy: Nucleic Acids, 2022
Gene editing utilizing homology-directed repair has advanced significantly for many monogenic diseases of the hematopoietic system in recent years but has also been hindered by decreases between in vitro and in vivo gene integration rates.
David H. Gray, Jasmine Santos, Alexandra Grace Keir, Isaac Villegas, Simon Maddock, Edward C. Trope, Joseph D. Long, Caroline Y. Kuo   +7 more
doaj   +1 more source

Microhomology-mediated end joining: new players join the team. [PDF]

Cell Biosci, 2017
DNA double-strand breaks (DSBs) are the most deleterious type of DNA damage in cells arising from endogenous and exogenous attacks on the genomic DNA. Timely and properly repair of DSBs is important for genomic integrity and survival. MMEJ is an error-prone repair mechanism for DSBs, which relies on exposed microhomologous sequence flanking broken ...
Wang H, Xu X.
europepmc   +4 more sources

Microhomology-Mediated End-Joining Chronicles: Tracing the Evolutionary Footprints of Genome Protection. [PDF]

Annu Rev Cell Dev Biol
The fidelity of genetic information is essential for cellular function and viability. DNA double-strand breaks (DSBs) pose a significant threat to genome integrity, necessitating efficient repair mechanisms. While the predominant repair strategies are usually accurate, paradoxically, error-prone pathways also exist. This review explores recent advances
Sfeir A, Tijsterman M, McVey M.
europepmc   +3 more sources

Protocol: A Multiplexed Reporter Assay to Study Effects of Chromatin Context on DNA Double-Strand Break Repair

Frontiers in Genetics, 2022
DNA double-strand breaks (DSBs) can be repaired through various pathways. Understanding how these pathways are regulated is of great interest for cancer research and optimization of gene editing.
Ruben Schep, Christ Leemans, Eva K. Brinkman, Tom van Schaik, Bas van Steensel, Bas van Steensel   +5 more
doaj   +1 more source

The APE2 nuclease is essential for DNA double-strand break repair by microhomology-mediated end joining. [PDF]

Mol Cell, 2023
Microhomology-mediated end joining (MMEJ) is an intrinsically mutagenic pathway of DNA double-strand break (DSB) repair essential for proliferation of homologous recombination (HR)-deficient tumors. Although targeting MMEJ has emerged as a powerful strategy to eliminate HR-deficient (HRD) cancers, this is limited by an incomplete understanding of the ...
Fleury H, MacEachern MK, Stiefel CM, Anand R, Sempeck C, Nebenfuehr B, Maurer-Alcalá K, Ball K, Proctor B, Belan O, Taylor E, Ortega R, Dodd B, Weatherly L, Dansoko D, Leung JW, Boulton SJ, Arnoult N.   +17 more
europepmc   +3 more sources

The APE2 nuclease is essential for DNA double strand break repair by microhomology-mediated end-joining. Fleury et al.

, 2023
Source data for "The APE2 nuclease is essential for DNA double strand break repair by microhomology-mediated end-joining." Fleury et al ...
Arnoult, N
core   +2 more sources