Results 41 to 50 of about 42,496 (156)

Helicase Q promotes homology-driven DNA double-strand break repair and prevents tandem duplications

Nature Communications, 2021
Microhomology-mediated end-joining (MMEJ) is a poorly defined mutagenic DNA break repair pathway. Here the authors show that the helicase HELQ is essential for polymerase theta-independent MMEJ, single-strand annealing and homologous recombination ...
J. A. Kamp, B. B. L. G. Lemmens, R. J. Romeijn, S. C. Changoer, R. van Schendel, M. Tijsterman   +5 more
doaj   +1 more source

PARP1 and PARP2 are dispensable for DNA repair by microhomology-mediated end-joining at double-ended DSBs. [PDF]

Nucleic Acids Res
Abstract Poly ADP-ribose polymerase (PARP) inhibitors are standard of care treatment for cancers with homologous-recombination deficiencies. Yet, as tumours develop resistance, complementary strategies are emerging, including targeting microhomology-mediated end joining (MMEJ). Given that PARP1 is widely described as a key promoter of
Ortega R, Taylor ER, Whitehead SM, Danhorn T, Bitler BG, Arnoult N.   +5 more
europepmc   +3 more sources

Rad52 mediates class-switch DNA recombination to IgD

Nature Communications, 2022
IgD is expressed, predominantly together with IgM, via mRNA alternative splicing, but IgD class switch recombination (IgD CSR) has also been reported. Here the authors show, using Rad52-deficient mouse and human B cells, that IgD CSR is mediated by Rad52
Yijiang Xu, Hang Zhou, Ginell Post, Hong Zan, Paolo Casali   +4 more
doaj   +1 more source

Efficient CRISPR/Cas9-based plant genomic fragment deletions by microhomology-mediated end joining. [PDF]

Plant Biotechnol J, 2020
Tan J, Zhao Y, Wang B, Hao Y, Wang Y, Li Y, Luo W, Zong W, Li G, Chen S, Ma K, Xie X, Chen L, Liu YG, Zhu Q.   +14 more
europepmc   +4 more sources

Complex Breakpoints and Template Switching Associated with Non-canonical Termination of Homologous Recombination in Mammalian Cells. [PDF]

PLoS Genetics, 2016
A proportion of homologous recombination (HR) events in mammalian cells resolve by "long tract" gene conversion, reflecting copying of several kilobases from the donor sister chromatid prior to termination.
Andrea J Hartlerode, Nicholas A Willis, Anbazhagan Rajendran, John P Manis, Ralph Scully   +4 more
doaj   +1 more source

53BP1 promotes microhomology-mediated end-joining in G1-phase cells. [PDF]

Nucleic Acids Res, 2015
Abstract Alternative non-homologous end joining (alt-NHEJ) was originally identified as a backup repair mechanism in the absence of classical NHEJ (c-NHEJ) factors but recent studies have demonstrated that alt-NHEJ is active even when c-NHEJ as well as homologous recombination is available.
Xiong X, Du Z, Wang Y, Feng Z, Fan P, Yan C, Willers H, Zhang J.   +7 more
europepmc   +4 more sources

CRISPR-Cas induced self-targeting identifies key players in archaeal microhomology-mediated end joining. [PDF]

Microlife
DNA repair processes are the foundation for genome integrity and survival, especially in extreme environments where DNA damage occurs more frequently and where archaea are found.
Sailer AL, Wörtz J, Smith V, Stachler AE, Blau F, Daratha M, Maier LK, Allers T, Marchfelder A.   +8 more
europepmc   +2 more sources

Microhomology-mediated mechanisms underlie non-recurrent disease-causing microdeletions of the FOXL2 gene or its regulatory domain. [PDF]

PLoS Genetics, 2013
Genomic disorders are often caused by recurrent copy number variations (CNVs), with nonallelic homologous recombination (NAHR) as the underlying mechanism.
Hannah Verdin, Barbara D'haene, Diane Beysen, Yana Novikova, Björn Menten, Tom Sante, Pablo Lapunzina, Julian Nevado, Claudia M B Carvalho, James R Lupski, Elfride De Baere   +10 more
doaj   +1 more source

Biased genome editing using the local accumulation of DSB repair molecules system

Nature Communications, 2018
Genome editing using CRISPR can be enhanced by manipulating DNA double-strand break repair pathways. Here the authors demonstrate LoAD, local accumulation of repair molecules, which shifts repair to microhomology-mediated end-joining.
Shota Nakade, Keiji Mochida, Atsushi Kunii, Kazuki Nakamae, Tomomi Aida, Kohichi Tanaka, Naoaki Sakamoto, Tetsushi Sakuma, Takashi Yamamoto   +8 more
doaj   +1 more source

Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations

Nature Communications, 2019
DNA repair by microhomology-mediated end joining creates precise deletions based on flanking microhomologies. Here the authors use CRISPR-Cas9 to recreate pathogenic deletion mutations using existing microhomologies in the human genome identified by ...
Janin Grajcarek, Jean Monlong, Yoko Nishinaka-Arai, Michiko Nakamura, Miki Nagai, Shiori Matsuo, David Lougheed, Hidetoshi Sakurai, Megumu K. Saito, Guillaume Bourque, Knut Woltjen   +10 more
doaj   +1 more source