Results 1 to 10 of about 10,180 (133)

Structural basis for Polθ-helicase DNA binding and microhomology-mediated end-joining [PDF]

Nature Communications
DNA double-strand breaks (DSBs) present a critical threat to genomic integrity, often precipitating genomic instability and oncogenesis. Repair of DSBs predominantly occurs through homologous recombination (HR) and non-homologous end joining (NHEJ).
Fumiaki Ito, Ziyuan Li, Leonid Minakhin, Htet A. Khant, Richard T. Pomerantz, Xiaojiang S. Chen   +5 more
doaj   +4 more sources

Translesion synthesis and microhomology-mediated end-joining repair in trypanosomatids. [PDF]

PLoS Neglected Tropical Diseases
Trypanosomatid parasites and the diseases they cause affect more than 30 million people annually worldwide. To develop treatments for these diseases, it is critical to understand how trypanosomatid biology protects the parasite, so that these mechanisms ...
Lea Drogalis Beckham, Anne Snyder, Sylvie Doublié, Bruno Martorelli Di Genova   +3 more
doaj   +3 more sources

Recurrent deletions in clonal hematopoiesis are driven by microhomology-mediated end joining [PDF]

Nature Communications, 2021
The mutational mechanisms that produce insertions and deletions that lead to clonal hematopoiesis are poorly understood. Here the authors show evidence that frequent deletions that are relevant to myeloid malignancies could be produced by PARP1-dependent
Tzah Feldman, Akhiad Bercovich, Yoni Moskovitz, Noa Chapal-Ilani, Amanda Mitchell, Jessie J. F. Medeiros, Tamir Biezuner, Nathali Kaushansky, Mark D. Minden, Vikas Gupta, Michael Milyavsky, Zvi Livneh, Amos Tanay, Liran I. Shlush   +13 more
doaj   +3 more sources

Molecular basis of microhomology-mediated end-joining by purified full-length Polθ [PDF]

Nature Communications, 2019
DNA polymerase θ is a polymerase-helicase essential for microhomology-mediated end-joining (MMEJ) or alternative end-joining of DNA. Here the authors use biochemical and biophysical methods to reveal how full-length human DNA polymerase θ performs MMEJ ...
Samuel J. Black, Ahmet Y. Ozdemir, Ekaterina Kashkina, Tatiana Kent, Timur Rusanov, Dejan Ristic, Yeonoh Shin, Antonio Suma, Trung Hoang, Gurushankar Chandramouly, Labiba A. Siddique, Nikita Borisonnik, Katherine Sullivan-Reed, Joseph S. Mallon, Tomasz Skorski, Vincenzo Carnevale, Katsuhiko S. Murakami, Claire Wyman, Richard T. Pomerantz   +18 more
doaj   +3 more sources

Optimizing CRISPR precision in mouse embryos via microhomology-mediated end joining-dominant targeting [PDF]

Communications Biology
CRISPR/Cas9 technology enables efficient gene editing in mice, but its reliance on non-homologous end joining often leads to unpredictable and mosaic mutations in founder (F0) animals.
Khanui Lkhagvadorj, Eiichi Okamura, Taito Taki, Hayate Suzuki, Akihiro Kuno, Yasushi Itoh, Seiya Mizuno, Knut Woltjen, Masatsugu Ema   +8 more
doaj   +3 more sources

Polθ promotes the repair of 5′-DNA-protein crosslinks by microhomology-mediated end-joining [PDF]

Cell Reports, 2021
Summary: DNA polymerase θ (Polθ) confers resistance to chemotherapy agents that cause DNA-protein crosslinks (DPCs) at double-strand breaks (DSBs), such as topoisomerase inhibitors. This suggests Polθ might facilitate DPC repair by microhomology-mediated
Gurushankar Chandramouly, Shuren Liao, Timur Rusanov, Nikita Borisonnik, Marissa L. Calbert, Tatiana Kent, Katherine Sullivan-Reed, Umeshkumar Vekariya, Ekaterina Kashkina, Tomasz Skorski, Hong Yan, Richard T. Pomerantz   +11 more
doaj   +3 more sources

Microhomology-mediated end joining induces hypermutagenesis at breakpoint junctions. [PDF]

PLoS Genetics, 2017
Microhomology (MH) flanking a DNA double-strand break (DSB) drives chromosomal rearrangements but its role in mutagenesis has not yet been analyzed. Here we determined the mutation frequency of a URA3 reporter gene placed at multiple locations distal to ...
Supriya Sinha, Fuyang Li, Diana Villarreal, Jae Hoon Shim, Suhyeon Yoon, Kyungjae Myung, Eun Yong Shim, Sang Eun Lee   +7 more
doaj   +4 more sources

Endogenous CRISPR-assisted microhomology-mediated end joining enables rapid genome editing in Zymomonas mobilis [PDF]

Biotechnology for Biofuels, 2021
Background Zymomonas mobilis is a natural ethanologen with many desirable characteristics, making it an ideal platform for future biorefineries. Recently, an endogenous CRISPR-based genome editing tool has been developed for this species.
Xin Sui, Xiaojie Wang, Tao Liu, Qing Ye, Bo Wu, Guoquan Hu, Shihui Yang, Mingxiong He, Nan Peng   +8 more
doaj   +3 more sources

Cyclin D1 overexpression induces replication stress and microhomology-mediated end-joining dependence in mantle cell lymphoma [PDF]

The Journal of Clinical Investigation
Oncogene expression can cause replication stress (RS), leading to DNA double-strand breaks (DSBs) that require repair through pathways such as homologous recombination, nonhomologous end-joining, and microhomology-mediated end-joining (MMEJ).
Jithma P. Abeykoon, Shuhei Asada, Guangli Zhu, Yuna Hirohashi, Lisa Moreau, Divya Iyer, Sirisha Mukkavalli, Kalindi Parmar, Gabriella Zambrano, Lige Jiang, Dongni Yi, Michelle Manske, Kimberly Gwin, Rebecca L. King, James R. Cerhan, Xiaosheng Wu, Zhenkun Lou, Geoffrey I. Shapiro, Thomas Witzig, Alan D’Andrea   +19 more
doaj   +3 more sources

A High-Efficiency CRISPR–Cas9 Ribonucleoprotein Genome Editing System in Aspergillus fijiensis Enabled by Microhomology-Mediated End Joining [PDF]

Journal of Fungi
Aspergillus fijiensis is an industrially important filamentous fungus, whose genetic analysis has been limited by the absence of species-specific tools. This study establishes an optimized CRISPR–Cas9 genome editing platform for A.
Zhenchun Duan, Shuangfei Zhang, Xueduan Liu   +2 more
doaj   +3 more sources