Robust activation of microhomology-mediated end joining for precision gene editing applications. [PDF]
PLoS Genetics, 2018 One key problem in precision genome editing is the unpredictable plurality of sequence outcomes at the site of targeted DNA double stranded breaks (DSBs).
Hirotaka Ata +9 more
doaj +8 more sources
Human papillomavirus 16 promotes microhomology-mediated end-joining [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2019 Significance Cancers associated with the human papillomavirus, including oropharyngeal, anal canal, cervical, and vulvar carcinomas, constitute about 4.5% of all solid tumors.
Jonathan E Leeman +2 more
exaly +6 more sources
Mouse papillomavirus type 1 (MmuPV1) DNA is frequently integrated in benign tumors by microhomology-mediated end-joining. [PDF]
PLoS Pathogens, 2021 MmuPV1 is a useful model for studying papillomavirus-induced tumorigenesis. We used RNA-seq to look for chimeric RNAs that map to both MmuPV1 and host genomes.
Lulu Yu +10 more
doaj +4 more sources
Microhomology-Mediated End Joining: A Back-up Survival Mechanism or Dedicated Pathway? [PDF]
Trends in Biochemical Sciences, 2015 DNA double-strand breaks (DSBs) disrupt the continuity of chromosomes and their repair by error-free mechanisms is essential to preserve genome integrity.
Agnel Sfeir, Lorraine S Symington
exaly +6 more sources
Polλ promotes microhomology-mediated end-joining. [PDF]
Nat Struct Mol Biol, 2023 Chandramouly et al. discover that human Polλ exhibits robust microhomology-mediated end-joining (MMEJ) activity like PolΦ. Polλ promotes MMEJ in mammalian cells independently of essential nonhomologous end-joining factors LIG4/XRCC4 and Polq, which ...
Chandramouly G +11 more
europepmc +4 more sources
Microhomology-Mediated End-Joining Chronicles: Tracing the Evolutionary Footprints of Genome Protection. [PDF]
Annu Rev Cell Dev BiolThe fidelity of genetic information is essential for cellular function and viability. DNA double-strand breaks (DSBs) pose a significant threat to genome integrity, necessitating efficient repair mechanisms.
Sfeir A, Tijsterman M, McVey M.
europepmc +4 more sources
Exploiting the Microhomology-Mediated End-Joining Pathway in Cancer Therapy. [PDF]
Cancer Res, 2020 Repair of DNA double-strand breaks (DSB) is performed by two major pathways, homology-dependent repair and classical nonhomologous end-joining. Recent studies have identified a third pathway, microhomology-mediated end-joining (MMEJ).
Patterson-Fortin J, D'Andrea AD.
europepmc +5 more sources
Comparison of Multiple Strategies for Precision Transgene Knock-In in Gallus gallus Genome via Microhomology-Mediated End Joining. [PDF]
Int J Mol Sci, 2023 Precision exogenous gene knock-in is an attractive field for transgenic Gallus gallus (chicken) generation. In this article, we constructed multiple Precise Integration into Target Chromosome (PITCh) plasmid systems mediated by microhomology-mediated end-
Wang L, Sun J, Liu Z, Zheng Q, Wang G.
europepmc +3 more sources
The APE2 nuclease is essential for DNA double-strand break repair by microhomology-mediated end joining. [PDF]
Mol Cell, 2023 Summary Microhomology-mediated end-joining (MMEJ) is an intrinsically mutagenic pathway of DNA double strand break (DSB) repair essential for proliferation of homologous recombination (HR) deficient tumors.
Fleury H +17 more
europepmc +4 more sources
CRISPR-Cas induced self-targeting identifies key players in archaeal microhomology-mediated end joining. [PDF]
MicrolifeDNA repair processes are the foundation for genome integrity and survival, especially in extreme environments where DNA damage occurs more frequently and where archaea are found.
Sailer AL +8 more
europepmc +4 more sources